##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        HphI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MslI        MspA1I      MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    StuI        StyI        SwaI        
TseI        TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI          761  GCGG                 CCGC(-3/-1)
AclI          740  AACGTT               AA^CGTT
AflIII       1216  ACATGT               A^C[GA][CT]GT
AlwI          779  GATCC                GGATC(4/5)
AvaII         443  GGTCC                G^G[AT]CC
BalI           64  TGGCCA               TGG^CCA
BbvCI        1118  CCTCAGC              CCTCAGC(-5/-2)
BsaWI         730  ACCGGA               [AT]^CCGG[AT]
BsiYI         434  CCTTTAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          434  CCTTTAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          757  GAATGC               GAATGC(1/-1)
Bsp1407I      827  TGTACA               T^GTACA
BspLU11I     1216  ACATGT               A^CATGT
BsrGI         827  TGTACA               T^GTACA
Cac8I         356  GCATGC               GC[ACGT]^[ACGT]GC
Cfr13I        443  GGTCC                G^G[ACGT]CC
EaeI           64  TGGCCA               [CT]^GGCC[GA]
Fnu4HI        761  GCGGC                GC^[ACGT]GC
HaeIII         65  GGCC                 GG^CC
HapII         731  CCGG                 C^CGG
HpaII         731  CCGG                 C^CGG
ItaI          761  GCGGC                GC^[ACGT]GC
MaeII         741  ACGT                 A^CGT
MaeIII        100  GTGAC                ^GT[ACGT]AC
MluNI          64  TGGCCA               TGG^CCA
MscI           64  TGGCCA               TGG^CCA
MspI          731  CCGG                 C^CGG
NdeI          422  CATATG               CA^TATG
Psp1406I      740  AACGTT               AA^CGTT
SapI          428  GAAGAGC              GCTCTTC(1/4)
Sau96I        443  GGTCC                G^G[ACGT]CC
SmlI          228  CTTGAG               C^T[CT][GA]AG
SphI          356  GCATGC               GCATG^C
SspBI         827  TGTACA               T^GTACA
SspI          237  AATATT               AAT^ATT
TaqI          616  TCGA                 T^CGA
Tsp45I        100  GTGAC                ^GT[GC]AC
TthHB8I       616  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BfaI          395  CTAG                 C^TAG
             1046  CTAG                
BsmAI           6  GAGAC                GTCTC(1/5)
              303  GAGAC               
DpnII         779  GATC                 ^GATC
             1283  GATC                
DraI          325  TTTAAA               TTT^AAA
              940  TTTAAA              
MaeI          395  CTAG                 C^TAG
             1046  CTAG                
MboI          779  GATC                 ^GATC
             1283  GATC                
NdeII         779  GATC                 ^GATC
             1283  GATC                
NspI          356  GCATGC               [GA]CATG^[CT]
             1216  ACATGT              
Sau3AI        779  GATC                 ^GATC
             1283  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BpmI        
BsrI        DdeI        EarI        EcoRI       HindIII     
HinfI       Ksp632I     MboII       MnlI        MseI        
NlaIII      RsaI        TfiI        Tru9I       Tsp509I