################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AspHI AvaI BanI BbeI BbvCI BcgI BclI BfrI BglI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I FauI FbaI FseI Hin1I HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AciI 299 CCGC CCGC(-3/-1) AhdI 1609 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1747 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 555 CCTGAGG CC^T[ACGT]AGG ApaI 1920 GGGCCC GGGCC^C AseI 1657 ATTAAT AT^TAAT AsnI 1657 ATTAAT AT^TAAT AspEI 1609 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1365 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1530 TGCGCA TGC^GCA AvrII 469 CCTAGG C^CTAGG BamHI 1309 GGATCC G^GATCC BanII 1920 GGGCCC G[GA]GC[CT]^C BbrPI 1165 CACGTG CAC^GTG BbsI 1594 GAAGAC GAAGAC(2/6) BciVI 1425 GTATCC GTATCC(6/5) BcnI 884 CCCGG CC^[GC]GG BglII 841 AGATCT A^GATCT BlnI 469 CCTAGG C^CTAGG BpuAI 1594 GAAGAC GAAGAC(2/6) BstBI 2681 TTCGAA TT^CGAA Bsu36I 555 CCTGAGG CC^T[ACGT]AGG DraI 1638 TTTAAA TTT^AAA DraIII 1930 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1609 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 555 CCTGAGG CC^T[ACGT]AGG EcoRI 133 GAATTC G^AATTC EcoRV 2345 GATATC GAT^ATC EcoT22I 2597 ATGCAT ATGCA^T FspI 1530 TGCGCA TGC^GCA HaeII 425 AGCGCC [GA]GCGC^[CT] HgaI 495 GACGC GACGC(5/10) HincII 2194 GTTGAC GT[CT]^[GA]AC HindII 2194 GTTGAC GT[CT]^[GA]AC HindIII 713 AAGCTT A^AGCTT MfeI 2219 CAATTG C^AATTG MunI 2219 CAATTG C^AATTG NciI 884 CCCGG CC^[GC]GG NcoI 1493 CCATGG C^CATGG NdeI 848 CATATG CA^TATG NsiI 2597 ATGCAT ATGCA^T NspI 2190 GCATGT [GA]CATG^[CT] NspV 2681 TTCGAA TT^CGAA PflFI 1365 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1165 CACGTG CAC^GTG PmlI 1165 CACGTG CAC^GTG PshBI 1657 ATTAAT AT^TAAT SapI 1449 GAAGAGC GCTCTTC(1/4) ScaI 2676 AGTACT AGT^ACT SfuI 2681 TTCGAA TT^CGAA SnaBI 587 TACGTA TAC^GTA StuI 1099 AGGCCT AGG^CCT SwaI 1637 ATTTAAAT ATTT^AAAT Tth111I 1365 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1887 TCTAGA T^CTAGA XcmI 2617 CCAAAATATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaII 1370 GGTCC G^G[AT]CC 2614 GGTCC BalI 215 TGGCCA TGG^CCA 1142 TGGCCA BlpI 1623 GCTCAGC GC^T[ACGT]AGC 2097 GCTGAGC Bpu1102I 1623 GCTCAGC GC^T[ACGT]AGC 2097 GCTGAGC BsgI 1744 GTGCAG GTGCAG(16/14) 2028 GTGCAG BsiYI 1256 CCCCCACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2546 CCCTTCATCGG BslI 1256 CCCCCACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2546 CCCTTCATCGG BsmI 2595 GAATGC GAATGC(1/-1) 2047 GCATTC BstYI 841 AGATCT [GA]^GATC[CT] 1309 GGATCC CelII 1623 GCTCAGC GC^T[ACGT]AGC 2097 GCTGAGC CfoI 426 GCGC GCG^C 1531 GCGC DraII 1325 AGGCCCC [GA]G^G[ACGT]CC[CT] 1919 GGGGCCC DsaI 1493 CCATGG C^C[GA][CT]GG 2199 CCGTGG EcoO109I 1325 AGGCCCC [GA]G^G[ACGT]CC[CT] 1919 GGGGCCC HapII 858 CCGG C^CGG 885 CCGG HhaI 426 GCGC GCG^C 1531 GCGC HinP1I 426 GCGC G^CGC 1531 GCGC HpaII 858 CCGG C^CGG 885 CCGG HphI 624 GGTGA GGTGA(8/7) 906 GGTGA MflI 841 AGATCT [GA]^GATC[CT] 1309 GGATCC MluNI 215 TGGCCA TGG^CCA 1142 TGGCCA MscI 215 TGGCCA TGG^CCA 1142 TGGCCA MspI 858 CCGG C^CGG 885 CCGG PleI 1684 GACTC GAGTC(4/5) 279 GAGTC XhoII 841 AGATCT [GA]^GATC[CT] 1309 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I BbvI BfaI BmyI BpmI BsaAI BsaJI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI