Restriction map for
pFN21AE4242
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspHI AvaI BanI BbeI BbvCI
BcgI BclI BfrI BglI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I FauI FbaI
FseI Hin1I HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspBI Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 299 CCGC CCGC(-3/-1)
AhdI 1609 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1747 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 555 CCTGAGG CC^T[ACGT]AGG
ApaI 1920 GGGCCC GGGCC^C
AseI 1657 ATTAAT AT^TAAT
AsnI 1657 ATTAAT AT^TAAT
AspEI 1609 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1365 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1530 TGCGCA TGC^GCA
AvrII 469 CCTAGG C^CTAGG
BamHI 1309 GGATCC G^GATCC
BanII 1920 GGGCCC G[GA]GC[CT]^C
BbrPI 1165 CACGTG CAC^GTG
BbsI 1594 GAAGAC GAAGAC(2/6)
BciVI 1425 GTATCC GTATCC(6/5)
BcnI 884 CCCGG CC^[GC]GG
BglII 841 AGATCT A^GATCT
BlnI 469 CCTAGG C^CTAGG
BpuAI 1594 GAAGAC GAAGAC(2/6)
BstBI 2681 TTCGAA TT^CGAA
Bsu36I 555 CCTGAGG CC^T[ACGT]AGG
DraI 1638 TTTAAA TTT^AAA
DraIII 1930 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1609 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 555 CCTGAGG CC^T[ACGT]AGG
EcoRI 133 GAATTC G^AATTC
EcoRV 2345 GATATC GAT^ATC
EcoT22I 2597 ATGCAT ATGCA^T
FspI 1530 TGCGCA TGC^GCA
HaeII 425 AGCGCC [GA]GCGC^[CT]
HgaI 495 GACGC GACGC(5/10)
HincII 2194 GTTGAC GT[CT]^[GA]AC
HindII 2194 GTTGAC GT[CT]^[GA]AC
HindIII 713 AAGCTT A^AGCTT
MfeI 2219 CAATTG C^AATTG
MunI 2219 CAATTG C^AATTG
NciI 884 CCCGG CC^[GC]GG
NcoI 1493 CCATGG C^CATGG
NdeI 848 CATATG CA^TATG
NsiI 2597 ATGCAT ATGCA^T
NspI 2190 GCATGT [GA]CATG^[CT]
NspV 2681 TTCGAA TT^CGAA
PflFI 1365 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1165 CACGTG CAC^GTG
PmlI 1165 CACGTG CAC^GTG
PshBI 1657 ATTAAT AT^TAAT
SapI 1449 GAAGAGC GCTCTTC(1/4)
ScaI 2676 AGTACT AGT^ACT
SfuI 2681 TTCGAA TT^CGAA
SnaBI 587 TACGTA TAC^GTA
StuI 1099 AGGCCT AGG^CCT
SwaI 1637 ATTTAAAT ATTT^AAAT
Tth111I 1365 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1887 TCTAGA T^CTAGA
XcmI 2617 CCAAAATATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 1370 GGTCC G^G[AT]CC
2614 GGTCC
BalI 215 TGGCCA TGG^CCA
1142 TGGCCA
BlpI 1623 GCTCAGC GC^T[ACGT]AGC
2097 GCTGAGC
Bpu1102I 1623 GCTCAGC GC^T[ACGT]AGC
2097 GCTGAGC
BsgI 1744 GTGCAG GTGCAG(16/14)
2028 GTGCAG
BsiYI 1256 CCCCCACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2546 CCCTTCATCGG
BslI 1256 CCCCCACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2546 CCCTTCATCGG
BsmI 2595 GAATGC GAATGC(1/-1)
2047 GCATTC
BstYI 841 AGATCT [GA]^GATC[CT]
1309 GGATCC
CelII 1623 GCTCAGC GC^T[ACGT]AGC
2097 GCTGAGC
CfoI 426 GCGC GCG^C
1531 GCGC
DraII 1325 AGGCCCC [GA]G^G[ACGT]CC[CT]
1919 GGGGCCC
DsaI 1493 CCATGG C^C[GA][CT]GG
2199 CCGTGG
EcoO109I 1325 AGGCCCC [GA]G^G[ACGT]CC[CT]
1919 GGGGCCC
HapII 858 CCGG C^CGG
885 CCGG
HhaI 426 GCGC GCG^C
1531 GCGC
HinP1I 426 GCGC G^CGC
1531 GCGC
HpaII 858 CCGG C^CGG
885 CCGG
HphI 624 GGTGA GGTGA(8/7)
906 GGTGA
MflI 841 AGATCT [GA]^GATC[CT]
1309 GGATCC
MluNI 215 TGGCCA TGG^CCA
1142 TGGCCA
MscI 215 TGGCCA TGG^CCA
1142 TGGCCA
MspI 858 CCGG C^CGG
885 CCGG
PleI 1684 GACTC GAGTC(4/5)
279 GAGTC
XhoII 841 AGATCT [GA]^GATC[CT]
1309 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BfaI BmyI BpmI
BsaAI BsaJI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI