Restriction map for
pFN21AE1907
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwNI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspHI BsrBI BsrDI BsrFI
BsrGI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI MaeI MamI MfeI MluNI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI ScaI SexAI SfiI
SfuI SgrAI SmaI SpeI SphI
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 145 GTAGAC GT^[AC][GT]AC
AccIII 1127 TCCGGA T^CCGGA
Aor51HI 494 AGCGCT AGC^GCT
BbeI 3 GGCGCC GGCGC^C
BcgI 1227 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 106 CCCGG CC^[GC]GG
BglI 1213 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 142 TACGTA [CT]AC^GT[GA]
BsaWI 1127 TCCGGA [AT]^CCGG[AT]
BseAI 1127 TCCGGA T^CCGGA
BsgI 844 GTGCAG GTGCAG(16/14)
BsiEI 108 CGGCCG CG[GA][CT]^CG
BsiWI 413 CGTACG C^GTACG
BsmBI 592 GAGACG CGTCTC(1/5)
BspEI 1127 TCCGGA T^CCGGA
BspLU11I 1166 ACATGT A^CATGT
BsrI 958 ACTGG ACTGG(1/-1)
BssHII 603 GCGCGC G^CGCGC
BssSI 850 CACGAG CACGAG(-5/-1)
EaeI 108 CGGCCG [CT]^GGCC[GA]
EagI 108 CGGCCG C^GGCCG
EclXI 108 CGGCCG C^GGCCG
Eco47III 494 AGCGCT AGC^GCT
Eco52I 108 CGGCCG C^GGCCG
EcoNI 438 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 1080 AAGCTT A^AGCTT
KasI 3 GGCGCC G^GCGCC
MluI 410 ACGCGT A^CGCGT
MroI 1127 TCCGGA T^CCGGA
MseI 1313 TTAA T^TAA
NarI 3 GGCGCC GG^CGCC
NciI 106 CCCGG CC^[GC]GG
PleI 1131 GAGTC GAGTC(4/5)
SacI 996 GAGCTC GAGCT^C
SbfI 766 CCTGCAGG CCTGCA^GG
SfaNI 920 GCATC GCATC(5/9)
SfoI 3 GGCGCC GGC^GCC
SmlI 75 CTTGAG C^T[CT][GA]AG
SnaBI 142 TACGTA TAC^GTA
Sse8387I 766 CCTGCAGG CCTGCA^GG
StuI 763 AGGCCT AGG^CCT
TaqI 170 TCGA T^CGA
TfiI 186 GATTC G^A[AT]TC
Tru9I 1313 TTAA T^TAA
Tsp509I 158 AATT ^AATT
TthHB8I 170 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 610 GATCC GGATC(4/5)
1172 GGATC
AocI 294 CCTGAGG CC^T[ACGT]AGG
663 CCTGAGG
AspHI 996 GAGCTC G[AT]GC[AT]^C
1099 GTGCTC
BsaI 757 GAGACC GGTCTC(1/5)
335 GGTCTC
BsiHKAI 996 GAGCTC G[AT]GC[AT]^C
1099 GTGCTC
BspMI 302 ACCTGC ACCTGC(4/8)
449 ACCTGC
BstAPI 156 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
622 GCACACGTTGC
BstYI 609 AGATCC [GA]^GATC[CT]
1172 GGATCT
Bsu36I 294 CCTGAGG CC^T[ACGT]AGG
663 CCTGAGG
EarI 1102 CTCTTC CTCTTC(1/4)
993 GAAGAG
Eco81I 294 CCTGAGG CC^T[ACGT]AGG
663 CCTGAGG
HaeII 3 GGCGCC [GA]GCGC^[CT]
494 AGCGCT
HinfI 186 GATTC G^A[ACGT]TC
1131 GAGTC
Ksp632I 1102 CTCTTC CTCTTC(1/4)
993 GAAGAG
MaeII 143 ACGT A^CGT
626 ACGT
MflI 609 AGATCC [GA]^GATC[CT]
1172 GGATCT
MslI 90 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
129 CATCGCCGTG
NspI 835 ACATGC [GA]CATG^[CT]
1166 ACATGT
PpuMI 446 AGGACCT [GA]G^G[AT]CC[CT]
532 AGGACCT
PstI 767 CTGCAG CTGCA^G
1031 CTGCAG
SapI 993 GAAGAGC GCTCTTC(1/4)
1101 GCTCTTC
XhoII 609 AGATCC [GA]^GATC[CT]
1172 GGATCT
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI ApaI AvaII BanI BanII
BbvI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI KspI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
SacII Sau3AI Sau96I ScrFI SfcI
StyI TseI Tsp45I TspRI XcmI