Restriction map for
pFN21AE5444
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvaI MvnI MwoI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 498 GGTACC G^GTACC
AccI 383 GTAGAC GT^[AC][GT]AC
AciI 439 GCGG CCGC(-3/-1)
AcsI 798 AAATTC [GA]^AATT[CT]
AflIII 721 ACATGT A^C[GA][CT]GT
AlwNI 241 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 261 GGGCCC GGGCC^C
ApoI 798 AAATTC [GA]^AATT[CT]
Asp700I 73 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 498 GGTACC G^GTACC
AspHI 609 GAGCTC G[AT]GC[AT]^C
AvaI 668 CCCGGG C^[CT]CG[GA]G
BbsI 28 GAAGAC GAAGAC(2/6)
BbvCI 64 CCTCAGC CCTCAGC(-5/-2)
BbvI 901 GCAGC GCAGC(8/12)
BclI 807 TGATCA T^GATCA
BfaI 520 CTAG C^TAG
BpmI 781 CTCCAG CTGGAG(16/14)
BpuAI 28 GAAGAC GAAGAC(2/6)
BsaAI 151 TACGTG [CT]AC^GT[GA]
BsiHKAI 609 GAGCTC G[AT]GC[AT]^C
BsmAI 909 GAGAC GTCTC(1/5)
BsoBI 668 CCCGGG C^[CT]CG[GA]G
BspHI 605 TCATGA T^CATGA
BspLU11I 721 ACATGT A^CATGT
BstF5I 141 CATCC GGATG(2/0)
BstYI 210 GGATCT [GA]^GATC[CT]
Cac8I 683 GCATGC GC[ACGT]^[ACGT]GC
DraII 260 GGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 906 GAAGAG CTCTTC(1/4)
EcoO109I 260 GGGGCCC [GA]G^G[ACGT]CC[CT]
FbaI 807 TGATCA T^GATCA
FokI 141 CATCC GGATG(9/13)
HincII 571 GTTGAC GT[CT]^[GA]AC
HindII 571 GTTGAC GT[CT]^[GA]AC
KpnI 498 GGTACC GGTAC^C
Ksp632I 906 GAAGAG CTCTTC(1/4)
MaeI 520 CTAG C^TAG
MaeII 152 ACGT A^CGT
MaeIII 844 GTCAC ^GT[ACGT]AC
MflI 210 GGATCT [GA]^GATC[CT]
MspA1I 67 CAGCTG C[AC]G^C[GT]G
NdeI 359 CATATG CA^TATG
PvuII 67 CAGCTG CAG^CTG
RcaI 605 TCATGA T^CATGA
SacI 609 GAGCTC GAGCT^C
SmaI 668 CCCGGG CCC^GGG
SphI 683 GCATGC GCATG^C
TseI 901 GCAGC G^C[AT]GC
Tsp45I 844 GTCAC ^GT[GC]AC
XhoII 210 GGATCT [GA]^GATC[CT]
XmaCI 668 CCCGGG C^CCGGG
XmaI 668 CCCGGG C^CCGGG
XmnI 73 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 210 GGATC GGATC(4/5)
525 GGATC
BanI 498 GGTACC G^G[CT][GA]CC
658 GGTGCC
BsaJI 258 CCGGGG C^C[ACGT][ACGT]GG
668 CCCGGG
BsrDI 663 CATTGC GCAATG(2/0)
822 CATTGC
Cfr13I 261 GGGCC G^G[ACGT]CC
262 GGCCC
Fnu4HI 439 GCGGC GC^[ACGT]GC
901 GCAGC
HaeIII 62 GGCC GG^CC
262 GGCC
HphI 249 GGTGA GGTGA(8/7)
351 GGTGA
ItaI 439 GCGGC GC^[ACGT]GC
901 GCAGC
MseI 8 TTAA T^TAA
857 TTAA
PflMI 426 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
732 CCATCTTATGG
PleI 157 GACTC GAGTC(4/5)
852 GAGTC
Sau96I 261 GGGCC G^G[ACGT]CC
262 GGCCC
TfiI 252 GAATC G^A[AT]TC
326 GAATC
Tru9I 8 TTAA T^TAA
857 TTAA
Van91I 426 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
732 CCATCTTATGG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI BanII BcnI BmyI
BsiYI BslI Bsp1286I BsrI BssKI
DdeI DpnII HapII HinfI HpaII
MboI MboII MnlI MslI MspI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI ScrFI Tsp509I TspRI