Restriction map for
pFN21AE5933
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI MwoI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 504 GGTACC G^GTACC
AccI 389 GTAGAC GT^[AC][GT]AC
AciI 522 CCGC CCGC(-3/-1)
AcsI 804 AAATTC [GA]^AATT[CT]
AflIII 727 ACATGT A^C[GA][CT]GT
AlwI 216 GGATC GGATC(4/5)
AlwNI 247 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 267 GGGCCC GGGCC^C
ApoI 804 AAATTC [GA]^AATT[CT]
Asp718I 504 GGTACC G^GTACC
AspHI 734 GAGCTC G[AT]GC[AT]^C
BbvI 379 GCAGC GCAGC(8/12)
BclI 608 TGATCA T^GATCA
BcnI 270 CCCGG CC^[GC]GG
BpmI 787 CTCCAG CTGGAG(16/14)
BsgI 172 CTGCAC GTGCAG(16/14)
BsiHKAI 734 GAGCTC G[AT]GC[AT]^C
BsmAI 915 GAGAC GTCTC(1/5)
BspHI 605 TCATGA T^CATGA
BspLU11I 727 ACATGT A^CATGT
BspMI 434 ACCTGC ACCTGC(4/8)
BsrDI 828 CATTGC GCAATG(2/0)
BstNI 159 CCTGG CC^[AT]GG
BstYI 216 GGATCT [GA]^GATC[CT]
DraII 266 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 336 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 770 CGGCCA [CT]^GGCC[GA]
EarI 912 GAAGAG CTCTTC(1/4)
EcoO109I 266 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 159 CCTGG ^CC[AT]GG
FbaI 608 TGATCA T^GATCA
Fnu4HI 379 GCAGC GC^[ACGT]GC
HincII 696 GTCAAC GT[CT]^[GA]AC
HindII 696 GTCAAC GT[CT]^[GA]AC
ItaI 379 GCAGC GC^[ACGT]GC
KpnI 504 GGTACC GGTAC^C
Ksp632I 912 GAAGAG CTCTTC(1/4)
MaeIII 850 GTCAC ^GT[ACGT]AC
MflI 216 GGATCT [GA]^GATC[CT]
MseI 863 TTAA T^TAA
MspA1I 380 CAGCTG C[AC]G^C[GT]G
MvaI 159 CCTGG CC^[AT]GG
NciI 270 CCCGG CC^[GC]GG
NheI 25 GCTAGC G^CTAGC
NspI 727 ACATGT [GA]CATG^[CT]
PflMI 432 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 163 GACTC GAGTC(4/5)
PvuII 380 CAGCTG CAG^CTG
RcaI 605 TCATGA T^CATGA
SacI 734 GAGCTC GAGCT^C
SfaNI 690 GATGC GCATC(5/9)
SmlI 809 CTCAAG C^T[CT][GA]AG
TfiI 303 GATTC G^A[AT]TC
Tru9I 863 TTAA T^TAA
TseI 379 GCAGC G^C[AT]GC
Tsp45I 850 GTCAC ^GT[GC]AC
Van91I 432 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 216 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanI 504 GGTACC G^G[CT][GA]CC
664 GGTGCC
BanII 267 GGGCCC G[GA]GC[CT]^C
734 GAGCTC
BfaI 26 CTAG C^TAG
857 CTAG
BmyI 267 GGGCCC G[AGT]GC[ACT]^C
734 GAGCTC
Bsp1286I 267 GGGCCC G[AGT]GC[ACT]^C
734 GAGCTC
BssKI 159 CCTGG ^CC[ACGT]GG
270 CCCGG
Cac8I 25 GCTAGC GC[ACGT]^[ACGT]GC
438 GCTGGC
Cfr13I 267 GGGCC G^G[ACGT]CC
268 GGCCC
HapII 271 CCGG C^CGG
769 CCGG
HinfI 163 GACTC G^A[ACGT]TC
303 GATTC
HpaII 271 CCGG C^CGG
769 CCGG
HphI 357 GGTGA GGTGA(8/7)
494 TCACC
MaeI 26 CTAG C^TAG
857 CTAG
MslI 177 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
586 CATGTCAATG
MspI 271 CCGG C^CGG
769 CCGG
Sau96I 267 GGGCC G^G[ACGT]CC
268 GGCCC
ScrFI 159 CCTGG CC^[ACGT]GG
270 CCCGG
Tsp509I 64 AATT ^AATT
805 AATT
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AfaI AluI BsiYI BslI BsrI
BstF5I DdeI DpnII FokI HaeIII
MboI MboII MnlI NdeII NlaIII
NlaIV RsaI Sau3AI TspRI