##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TaqI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          215  GAATTT               [GA]^AATT[CT]
AlwNI          68  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          193  CCTAAGG              CC^T[ACGT]AGG
ApoI          215  GAATTT               [GA]^AATT[CT]
AvaI          413  CTCGGG               C^[CT]CG[GA]G
BbvI          245  GCAGC                GCAGC(8/12)
BsaBI         334  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          278  GGTCTC               GGTCTC(1/5)
BsiYI         130  CCCTATCAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          130  CCCTATCAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         279  GTCTC                GTCTC(1/5)
BsoBI         413  CTCGGG               C^[CT]CG[GA]G
BspMI          67  GCAGGT               ACCTGC(4/8)
BstF5I        223  CATCC                GGATG(2/0)
BstYI         374  GGATCT               [GA]^GATC[CT]
Bsu36I        193  CCTAAGG              CC^T[ACGT]AGG
Cac8I          99  GCAAGC               GC[ACGT]^[ACGT]GC
EarI            9  GAAGAG               CTCTTC(1/4)
Eco81I        193  CCTAAGG              CC^T[ACGT]AGG
Fnu4HI        245  GCAGC                GC^[ACGT]GC
FokI          223  CATCC                GGATG(9/13)
HaeIII        116  GGCC                 GG^CC
ItaI          245  GCAGC                GC^[ACGT]GC
Ksp632I         9  GAAGAG               CTCTTC(1/4)
MamI          334  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII           9  GAAGA                GAAGA(8/7)
MflI          374  GGATCT               [GA]^GATC[CT]
MnlI           49  CCTC                 CCTC(7/6)
MseI          233  TTAA                 T^TAA
PleI          423  GACTC                GAGTC(4/5)
ScaI          303  AGTACT               AGT^ACT
SspI          229  AATATT               AAT^ATT
TfiI           14  GAATC                G^A[AT]TC
Tru9I         233  TTAA                 T^TAA
TseI          245  GCAGC                G^C[AT]GC
XhoII         374  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AfaI          304  GTAC                 GT^AC
              399  GTAC                
AluI          102  AGCT                 AG^CT
              411  AGCT                
AvaII          45  GGACC                G^G[AT]CC
               70  GGTCC               
BpmI           50  CTCCAG               CTGGAG(16/14)
              281  CTCCAG              
BsmFI          44  GGGAC                GGGAC(10/14)
              128  GTCCC               
DraII          44  GGGACCC              [GA]G^G[ACGT]CC[CT]
               69  AGGTCCT             
EcoO109I       44  GGGACCC              [GA]G^G[ACGT]CC[CT]
               69  AGGTCCT             
HinfI          14  GAATC                G^A[ACGT]TC
              423  GACTC               
PpuMI          44  GGGACCC              [GA]G^G[AT]CC[CT]
               69  AGGTCCT             
RsaI          304  GTAC                 GT^AC
              399  GTAC                
TspRI          58  CAGTG                CA[GC]TG[ACGT][ACGT]^
              274  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AlwI        Cfr13I      DdeI        DpnII       MboI        
NdeII       NlaIV       Sau3AI      Sau96I      Tsp509I