Restriction map for
pFN21AE2137
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BalI BamHI BbeI
BbrPI BbvCI BcgI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MslI MunI NarI
NdeI NheI NotI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 2272 CGCG CG^CG
AcyI 363 GGCGTC G[GA]^CG[CT]C
AflII 321 CTTAAG C^TTAAG
AflIII 1215 ACATGT A^C[GA][CT]GT
AocI 760 CCTCAGG CC^T[ACGT]AGG
ApaI 658 GGGCCC GGGCC^C
Asp700I 627 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1126 GAGCTC G[AT]GC[AT]^C
AvaI 965 CCCGGG C^[CT]CG[GA]G
AvrII 289 CCTAGG C^CTAGG
BanI 698 GGCACC G^G[CT][GA]CC
BclI 881 TGATCA T^GATCA
BfrI 321 CTTAAG C^TTAAG
BglI 726 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1013 AGATCT A^GATCT
BlnI 289 CCTAGG C^CTAGG
BsaBI 1300 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 363 GGCGTC G[GA]^CG[CT]C
BsaI 193 GGTCTC GGTCTC(1/5)
BsiHKAI 1126 GAGCTC G[AT]GC[AT]^C
BsoBI 965 CCCGGG C^[CT]CG[GA]G
BspLU11I 1215 ACATGT A^CATGT
BspMI 1027 ACCTGC ACCTGC(4/8)
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BstUI 2272 CGCG CG^CG
Bsu36I 760 CCTCAGG CC^T[ACGT]AGG
EaeI 1083 CGGCCA [CT]^GGCC[GA]
Eco81I 760 CCTCAGG CC^T[ACGT]AGG
EcoT22I 566 ATGCAT ATGCA^T
FbaI 881 TGATCA T^GATCA
HgaI 364 GCGTC GACGC(5/10)
Hin1I 363 GGCGTC G[GA]^CG[CT]C
HincII 1211 GTCAAC GT[CT]^[GA]AC
HindII 1211 GTCAAC GT[CT]^[GA]AC
MamI 1300 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2272 CGCG CG^CG
NruI 2271 TCGCGA TCG^CGA
NsiI 566 ATGCAT ATGCA^T
NspI 1215 ACATGT [GA]CATG^[CT]
PflMI 1086 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 642 CAGCTG CAG^CTG
SacI 1126 GAGCTC GAGCT^C
SapI 976 GAAGAGC GCTCTTC(1/4)
SexAI 986 ACCTGGT A^CC[AT]GGT
SmaI 965 CCCGGG CCC^GGG
TaqI 207 TCGA T^CGA
TthHB8I 207 TCGA T^CGA
Van91I 1086 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1439 CCAAAGGACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 965 CCCGGG C^CCGGG
XmaI 965 CCCGGG C^CCGGG
XmnI 627 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 411 AAATTT [GA]^AATT[CT]
1201 AAATTT
AfaI 476 GTAC GT^AC
1002 GTAC
AlwNI 982 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1087 CAGGAACTG
ApoI 411 AAATTT [GA]^AATT[CT]
1201 AAATTT
BbsI 37 GAAGAC GAAGAC(2/6)
844 GAAGAC
BciVI 868 GGATAC GTATCC(6/5)
1715 GGATAC
BcnI 965 CCCGG CC^[GC]GG
966 CCGGG
BpmI 247 CTCCAG CTGGAG(16/14)
464 CTGGAG
BpuAI 37 GAAGAC GAAGAC(2/6)
844 GAAGAC
BsgI 811 CTGCAC GTGCAG(16/14)
683 GTGCAG
BsmI 353 GAATGC GAATGC(1/-1)
420 GAATGC
BsrFI 1853 GCCGGC [GA]^CCGG[CT]
1937 GCCGGC
Cfr10I 1853 GCCGGC [GA]^CCGG[CT]
1937 GCCGGC
DraII 658 GGGCCCC [GA]G^G[ACGT]CC[CT]
1420 AGGGCCT
DsaI 65 CCATGG C^C[GA][CT]GG
654 CCATGG
EcoO109I 658 GGGCCCC [GA]G^G[ACGT]CC[CT]
1420 AGGGCCT
MseI 322 TTAA T^TAA
1604 TTAA
MspA1I 642 CAGCTG C[AC]G^C[GT]G
802 CCGCTG
MwoI 726 GCCCCCAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
804 GCTGGGTCTGC
NaeI 1853 GCCGGC GCC^GGC
1937 GCCGGC
NciI 965 CCCGG CC^[GC]GG
966 CCGGG
NcoI 65 CCATGG C^CATGG
654 CCATGG
NgoMIV 1853 GCCGGC G^CCGGC
1937 GCCGGC
PleI 1034 GAGTC GAGTC(4/5)
1489 GAGTC
RsaI 476 GTAC GT^AC
1002 GTAC
Tru9I 322 TTAA T^TAA
1604 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI TseI Tsp45I
Tsp509I TspRI XhoII