Restriction map for
pFN21AE9458
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AlwNI AocI
ApaI AscI Asp718I BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlpI Bpu1102I BsaI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I DrdI
EagI EclXI Eco52I Eco81I EcoNI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII SacII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SpeI SphI SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflIII 118 ACATGT A^C[GA][CT]GT
AhdI 1894 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 312 GTGCAC G^TGCAC
ApaLI 312 GTGCAC G^TGCAC
AseI 2053 ATTAAT AT^TAAT
AsnI 2053 ATTAAT AT^TAAT
Asp700I 924 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1894 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 774 CCCGAG C^[CT]CG[GA]G
AviII 527 TGCGCA TGC^GCA
AvrII 1995 CCTAGG C^CTAGG
BanII 807 GAGCTC G[GA]GC[CT]^C
BbeI 517 GGCGCC GGCGC^C
BbsI 1538 GAAGAC GAAGAC(2/6)
BglI 844 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1995 CCTAGG C^CTAGG
BpuAI 1538 GAAGAC GAAGAC(2/6)
BsaAI 568 TACGTA [CT]AC^GT[GA]
BsaBI 1943 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1627 TCCGGT [AT]^CCGG[AT]
BsmI 1657 GCATTC GAATGC(1/-1)
BsoBI 774 CCCGAG C^[CT]CG[GA]G
BspDI 1775 ATCGAT AT^CGAT
BspLU11I 118 ACATGT A^CATGT
BssSI 613 CTCGTG CACGAG(-5/-1)
BstEII 252 GGTGACC G^GT[ACGT]ACC
BstPI 252 GGTGACC G^GT[ACGT]ACC
ClaI 1775 ATCGAT AT^CGAT
CpoI 825 CGGACCG CG^G[AT]CCG
DraI 75 TTTAAA TTT^AAA
DraIII 985 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 699 CCGTGG C^C[GA][CT]GG
Eam1105I 1894 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 252 GGTGACC G^GT[ACGT]ACC
EcoRI 1192 GAATTC G^AATTC
FspI 527 TGCGCA TGC^GCA
HindIII 2156 AAGCTT A^AGCTT
KasI 517 GGCGCC G^GCGCC
MamI 1943 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 517 GGCGCC GG^CGCC
NdeI 1145 CATATG CA^TATG
NspI 118 ACATGT [GA]CATG^[CT]
PflFI 1668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1091 CCAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2053 ATTAAT AT^TAAT
RsrII 825 CGGACCG CG^G[AT]CCG
SacI 807 GAGCTC GAGCT^C
SbfI 860 CCTGCAGG CCTGCA^GG
SfoI 517 GGCGCC GGC^GCC
SnaBI 568 TACGTA TAC^GTA
Sse8387I 860 CCTGCAGG CCTGCA^GG
StuI 1886 AGGCCT AGG^CCT
TaqI 1776 TCGA T^CGA
Tth111I 1668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1776 TCGA T^CGA
Van91I 1091 CCAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 924 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 517 GGCGCC G[GA]^CG[CT]C
1155 GGCGTC
AfaI 571 GTAC GT^AC
591 GTAC
AlwI 939 GATCC GGATC(4/5)
1778 GATCC
Aor51HI 926 AGCGCT AGC^GCT
945 AGCGCT
BanI 517 GGCGCC G^G[CT][GA]CC
892 GGCACC
BglII 1569 AGATCT A^GATCT
1942 AGATCT
BsaHI 517 GGCGCC G[GA]^CG[CT]C
1155 GGCGTC
BseRI 610 CTCCTC GAGGAG(10/8)
658 GAGGAG
BstXI 203 CCATGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1823 CCACGTTTGTGG
BstYI 1569 AGATCT [GA]^GATC[CT]
1942 AGATCT
EarI 1552 GAAGAG CTCTTC(1/4)
1882 GAAGAG
Eco47III 926 AGCGCT AGC^GCT
945 AGCGCT
HgaI 242 GCGTC GACGC(5/10)
1156 GCGTC
Hin1I 517 GGCGCC G[GA]^CG[CT]C
1155 GGCGTC
Ksp632I 1552 GAAGAG CTCTTC(1/4)
1882 GAAGAG
MflI 1569 AGATCT [GA]^GATC[CT]
1942 AGATCT
PpuMI 287 AGGTCCC [GA]G^G[AT]CC[CT]
718 GGGACCC
PstI 861 CTGCAG CTGCA^G
1131 CTGCAG
RsaI 571 GTAC GT^AC
591 GTAC
Tsp45I 253 GTGAC ^GT[GC]AC
1991 GTGAC
XhoII 1569 AGATCT [GA]^GATC[CT]
1942 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
AvaII BalI BbvI BcnI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RcaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI XcmI