Restriction map for
pFN21AB8967
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HapII HincII HindII HpaI HpaII
Ksp632I KspI MamI MflI MroI
MspI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI TfiI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 419 GGTACC G^GTACC
AccII 247 CGCG CG^CG
AciI 972 CCGC CCGC(-3/-1)
AcsI 347 AAATTC [GA]^AATT[CT]
AflIII 246 ACGCGT A^C[GA][CT]GT
AlwI 334 GATCC GGATC(4/5)
ApoI 347 AAATTC [GA]^AATT[CT]
Asp718I 419 GGTACC G^GTACC
BanII 317 GGGCTC G[GA]GC[CT]^C
BbeI 789 GGCGCC GGCGC^C
BfaI 424 CTAG C^TAG
BglI 511 GCCATTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 94 CTGGAG CTGGAG(16/14)
BsaAI 535 TACGTG [CT]AC^GT[GA]
BseRI 462 GAGGAG GAGGAG(10/8)
BsgI 1022 CTGCAC GTGCAG(16/14)
BsmFI 949 GGGAC GGGAC(10/14)
Bsp1407I 377 TGTACA T^GTACA
BsrDI 114 GCAATG GCAATG(2/0)
BsrGI 377 TGTACA T^GTACA
BsrI 93 ACTGG ACTGG(1/-1)
BstF5I 63 GGATG GGATG(2/0)
BstUI 247 CGCG CG^CG
CfoI 790 GCGC GCG^C
Cfr13I 372 GGGCC G^G[ACGT]CC
DraI 639 TTTAAA TTT^AAA
FokI 63 GGATG GGATG(9/13)
HaeII 789 GGCGCC [GA]GCGC^[CT]
HgaI 130 GACGC GACGC(5/10)
HhaI 790 GCGC GCG^C
HinP1I 790 GCGC G^CGC
HindIII 748 AAGCTT A^AGCTT
HinfI 239 GACTC G^A[ACGT]TC
HphI 88 GGTGA GGTGA(8/7)
KasI 789 GGCGCC G^GCGCC
KpnI 419 GGTACC GGTAC^C
MaeI 424 CTAG C^TAG
MaeII 536 ACGT A^CGT
MfeI 396 CAATTG C^AATTG
MluI 246 ACGCGT A^CGCGT
MslI 1059 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 396 CAATTG C^AATTG
MvnI 247 CGCG CG^CG
NarI 789 GGCGCC GG^CGCC
PleI 239 GACTC GAGTC(4/5)
Sau96I 372 GGGCC G^G[ACGT]CC
SfoI 789 GGCGCC GGC^GCC
SspBI 377 TGTACA T^GTACA
SspI 799 AATATT AAT^ATT
TaqI 1036 TCGA T^CGA
TthHB8I 1036 TCGA T^CGA
XcmI 41 CCAATTACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 130 GACGCC G[GA]^CG[CT]C
789 GGCGCC
BalI 38 TGGCCA TGG^CCA
518 TGGCCA
BbsI 559 GAAGAC GAAGAC(2/6)
772 GTCTTC
BbvI 857 GCAGC GCAGC(8/12)
914 GCTGC
BmyI 317 GGGCTC G[AGT]GC[ACT]^C
469 GTGCCC
BpuAI 559 GAAGAC GAAGAC(2/6)
772 GTCTTC
BsaHI 130 GACGCC G[GA]^CG[CT]C
789 GGCGCC
BsiYI 458 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
495 CCTGGCATGGG
BslI 458 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
495 CCTGGCATGGG
BsmAI 385 GAGAC GTCTC(1/5)
613 GAGAC
Bsp1286I 317 GGGCTC G[AGT]GC[ACT]^C
469 GTGCCC
Cac8I 51 GCTGGC GC[ACGT]^[ACGT]GC
603 GCCAGC
EaeI 38 TGGCCA [CT]^GGCC[GA]
518 TGGCCA
Hin1I 130 GACGCC G[GA]^CG[CT]C
789 GGCGCC
MluNI 38 TGGCCA TGG^CCA
518 TGGCCA
MscI 38 TGGCCA TGG^CCA
518 TGGCCA
MseI 19 TTAA T^TAA
640 TTAA
PvuII 151 CAGCTG CAG^CTG
605 CAGCTG
SfaNI 479 GCATC GCATC(5/9)
917 GCATC
Tru9I 19 TTAA T^TAA
640 TTAA
TseI 857 GCAGC G^C[AT]GC
914 GCTGC
Tsp45I 119 GTGAC ^GT[GC]AC
845 GTGAC
TspRI 381 CAGTG CA[GC]TG[ACGT][ACGT]^
910 CACTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AluI BanI BsaJI BssKI
BstNI DdeI DpnII EcoRII Fnu4HI
HaeIII ItaI MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
Tsp509I