Restriction map for
pFN21AE3396
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AspI AviII BamHI BbeI
BbvCI BcgI BfrI BglI BsaHI
BsaI BseAI BsiEI BsiWI BspDI
BspEI BsrFI BssHII Bst1107I BstZ17I
CfoI Cfr10I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KspI MluI MroI NaeI NarI
NcoI NgoMIV NheI NotI PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 1958 GGTACC G^GTACC
AflIII 2126 ACATGT A^C[GA][CT]GT
AhdI 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2602 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1958 GGTACC G^GTACC
AspEI 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1445 CCCGAG C^[CT]CG[GA]G
AvrII 370 CCTAGG C^CTAGG
BalI 1502 TGGCCA TGG^CCA
BbrPI 1594 CACGTG CAC^GTG
BciVI 2748 GTATCC GTATCC(6/5)
BclI 3677 TGATCA T^GATCA
BcnI 3573 CCCGG CC^[GC]GG
BlnI 370 CCTAGG C^CTAGG
BlpI 1624 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1624 GCTGAGC GC^T[ACGT]AGC
BsaAI 1594 CACGTG [CT]AC^GT[GA]
BsaBI 2328 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3367 TCCGGT [AT]^CCGG[AT]
BsmBI 142 CGTCTC CGTCTC(1/5)
BsmFI 2070 GGGAC GGGAC(10/14)
BsoBI 1445 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2330 TGTACA T^GTACA
BspHI 183 TCATGA T^CATGA
BspLU11I 2126 ACATGT A^CATGT
BsrBI 3742 GAGCGG CCGCTC(-3/-3)
BsrDI 2384 GCAATG GCAATG(2/0)
BsrGI 2330 TGTACA T^GTACA
BssSI 2884 CTCGTG CACGAG(-5/-1)
BstBI 3198 TTCGAA TT^CGAA
BstEII 1489 GGTCACC G^GT[ACGT]ACC
BstPI 1489 GGTCACC G^GT[ACGT]ACC
CelII 1624 GCTGAGC GC^T[ACGT]AGC
DsaI 2777 CCACGG C^C[GA][CT]GG
EaeI 1502 TGGCCA [CT]^GGCC[GA]
Eam1105I 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1489 GGTCACC G^GT[ACGT]ACC
EcoT22I 2238 ATGCAT ATGCA^T
FauI 3732 GCGGG CCCGC(4/6)
FbaI 3677 TGATCA T^GATCA
HgaI 875 GCGTC GACGC(5/10)
KpnI 1958 GGTACC GGTAC^C
MamI 2328 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1502 TGGCCA TGG^CCA
MscI 1502 TGGCCA TGG^CCA
NciI 3573 CCCGG CC^[GC]GG
NdeI 3017 CATATG CA^TATG
NruI 3038 TCGCGA TCG^CGA
NsiI 2238 ATGCAT ATGCA^T
NspV 3198 TTCGAA TT^CGAA
PmaCI 1594 CACGTG CAC^GTG
PmlI 1594 CACGTG CAC^GTG
PshAI 45 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 183 TCATGA T^CATGA
SacI 1627 GAGCTC GAGCT^C
SfuI 3198 TTCGAA TT^CGAA
SphI 3350 GCATGC GCATG^C
SspBI 2330 TGTACA T^GTACA
StuI 118 AGGCCT AGG^CCT
XcmI 3266 CCACACTGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2602 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 959 GTAGAC GT^[AC][GT]AC
2650 GTAGAC
AccII 2290 CGCG CG^CG
3039 CGCG
AlwNI 1207 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2434 CAGGAGCTG
AocI 1036 CCTAAGG CC^T[ACGT]AGG
3421 CCTCAGG
AseI 760 ATTAAT AT^TAAT
1677 ATTAAT
AsnI 760 ATTAAT AT^TAAT
1677 ATTAAT
BanI 1832 GGTGCC G^G[CT][GA]CC
1958 GGTACC
BanII 1627 GAGCTC G[GA]GC[CT]^C
3734 GGGCTC
BbsI 1161 GAAGAC GAAGAC(2/6)
3433 GAAGAC
BpuAI 1161 GAAGAC GAAGAC(2/6)
3433 GAAGAC
BsgI 231 CTGCAC GTGCAG(16/14)
2431 GTGCAG
BspMI 3248 ACCTGC ACCTGC(4/8)
3437 ACCTGC
BstUI 2290 CGCG CG^CG
3039 CGCG
Bsu36I 1036 CCTAAGG CC^T[ACGT]AGG
3421 CCTCAGG
DraIII 2425 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3267 CACACTGTG
DrdI 1193 GACACCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3227 GACTTGTCAGTC
Eco81I 1036 CCTAAGG CC^T[ACGT]AGG
3421 CCTCAGG
HapII 3368 CCGG C^CGG
3574 CCGG
HpaII 3368 CCGG C^CGG
3574 CCGG
MaeII 1595 ACGT A^CGT
2490 ACGT
MspI 3368 CCGG C^CGG
3574 CCGG
MvnI 2290 CGCG CG^CG
3039 CGCG
PshBI 760 ATTAAT AT^TAAT
1677 ATTAAT
SapI 1428 GAAGAGC GCTCTTC(1/4)
1849 GAAGAGC
SspI 169 AATATT AAT^ATT
1323 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BglII BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII