Restriction map for
pFN21AE9504
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI Hin1I HindIII HinfI HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacII SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 445 GTGCAC G^TGCAC
AlwNI 835 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 79 AGCGCT AGC^GCT
ApaI 112 GGGCCC GGGCC^C
ApaLI 445 GTGCAC G^TGCAC
BbrPI 880 CACGTG CAC^GTG
BpmI 1007 CTGGAG CTGGAG(16/14)
BsgI 832 GTGCAG GTGCAG(16/14)
BsmAI 407 GTCTC GTCTC(1/5)
BspLU11I 386 ACATGT A^CATGT
DraII 111 GGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 283 CTCTTC CTCTTC(1/4)
Eco47III 79 AGCGCT AGC^GCT
EcoO109I 111 GGGGCCC [GA]G^G[ACGT]CC[CT]
Ksp632I 283 CTCTTC CTCTTC(1/4)
MslI 204 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 579 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 880 CACGTG CAC^GTG
PmlI 880 CACGTG CAC^GTG
SacI 280 GAGCTC GAGCT^C
SalI 373 GTCGAC G^TCGAC
SapI 282 GCTCTTC GCTCTTC(1/4)
SfiI 309 GGCCTCGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1051 CTCAAG C^T[CT][GA]AG
Van91I 579 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 607 CCACACCCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 373 GTCGAC GT^[AC][GT]AC
637 GTCTAC
AflIII 386 ACATGT A^C[GA][CT]GT
881 ACGTGT
AlwI 213 GATCC GGATC(4/5)
959 GATCC
AvaI 25 CCCGGG C^[CT]CG[GA]G
115 CCCGGG
AvaII 426 GGACC G^G[AT]CC
1151 GGACC
BbvCI 894 CCTCAGC CCTCAGC(-5/-2)
1015 GCTGAGG
BglI 310 GCCTCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
616 GCCTGGTCGGC
BsiEI 317 CGGCCG CG[GA][CT]^CG
1125 CGGCCG
BsoBI 25 CCCGGG C^[CT]CG[GA]G
115 CCCGGG
BsrFI 470 ACCGGC [GA]^CCGG[CT]
564 ACCGGC
BstAPI 64 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
447 GCACCCTCTGC
Cfr10I 470 ACCGGC [GA]^CCGG[CT]
564 ACCGGC
DpnII 213 GATC ^GATC
959 GATC
EagI 317 CGGCCG C^GGCCG
1125 CGGCCG
EclXI 317 CGGCCG C^GGCCG
1125 CGGCCG
Eco52I 317 CGGCCG C^GGCCG
1125 CGGCCG
HincII 373 GTCGAC GT[CT]^[GA]AC
874 GTCAAC
HindII 373 GTCGAC GT[CT]^[GA]AC
874 GTCAAC
MaeIII 529 GTCAC ^GT[ACGT]AC
853 GTGAC
MboI 213 GATC ^GATC
959 GATC
NdeII 213 GATC ^GATC
959 GATC
NspI 386 ACATGT [GA]CATG^[CT]
1076 GCATGT
PstI 62 CTGCAG CTGCA^G
598 CTGCAG
PvuII 835 CAGCTG CAG^CTG
909 CAGCTG
Sau3AI 213 GATC ^GATC
959 GATC
SfcI 62 CTGCAG C^T[GA][CT]AG
598 CTGCAG
SmaI 25 CCCGGG CCC^GGG
115 CCCGGG
TaqI 13 TCGA T^CGA
374 TCGA
Tsp45I 529 GTCAC ^GT[GC]AC
853 GTGAC
TthHB8I 13 TCGA T^CGA
374 TCGA
XmaCI 25 CCCGGG C^CCGGG
115 CCCGGG
XmaI 25 CCCGGG C^CCGGG
115 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AspHI BalI
BanII BbsI BbvI BcnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI TseI
TspRI