Restriction map for
pFN21AE0789
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BsrI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
HphI KpnI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI SacI SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 601 GTCTAC GT^[AC][GT]AC
AgeI 719 ACCGGT A^CCGGT
AlwI 675 GATCC GGATC(4/5)
AscI 97 GGCGCGCC GG^CGCGCC
BalI 665 TGGCCA TGG^CCA
BglII 701 AGATCT A^GATCT
BsaI 64 GAGACC GGTCTC(1/5)
BsaWI 719 ACCGGT [AT]^CCGG[AT]
BsmBI 294 CGTCTC CGTCTC(1/5)
BsmFI 810 GTCCC GGGAC(10/14)
BstF5I 45 CATCC GGATG(2/0)
BstYI 701 AGATCT [GA]^GATC[CT]
CpoI 897 CGGTCCG CG^G[AT]CCG
DraII 160 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 583 CTCTTC CTCTTC(1/4)
EcoO109I 160 GGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 45 CATCC GGATG(9/13)
Ksp632I 583 CTCTTC CTCTTC(1/4)
MaeII 1057 ACGT A^CGT
MaeIII 218 GTCAC ^GT[ACGT]AC
MboII 584 TCTTC GAAGA(8/7)
MflI 701 AGATCT [GA]^GATC[CT]
MluNI 665 TGGCCA TGG^CCA
MscI 665 TGGCCA TGG^CCA
PflMI 398 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 719 ACCGGT A^CCGGT
PstI 766 CTGCAG CTGCA^G
RsrII 897 CGGTCCG CG^G[AT]CCG
SapI 582 GCTCTTC GCTCTTC(1/4)
SfaNI 503 GCATC GCATC(5/9)
SfcI 766 CTGCAG C^T[GA][CT]AG
SmaI 972 CCCGGG CCC^GGG
TaqI 59 TCGA T^CGA
Tsp45I 218 GTCAC ^GT[GC]AC
TthHB8I 59 TCGA T^CGA
Van91I 398 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 701 AGATCT [GA]^GATC[CT]
XmaCI 972 CCCGGG C^CCGGG
XmaI 972 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 580 GTGCTC G[AT]GC[AT]^C
658 GAGCAC
AvaI 649 CCCGAG C^[CT]CG[GA]G
972 CCCGGG
AvaII 423 GGACC G^G[AT]CC
898 GGTCC
BsgI 535 CTGCAC GTGCAG(16/14)
964 CTGCAC
BsiHKAI 580 GTGCTC G[AT]GC[AT]^C
658 GAGCAC
BsmAI 64 GAGAC GTCTC(1/5)
295 GTCTC
BsoBI 649 CCCGAG C^[CT]CG[GA]G
972 CCCGGG
BsrBI 557 CCGCTC CCGCTC(-3/-3)
613 GAGCGG
BssHII 98 GCGCGC G^CGCGC
146 GCGCGC
BstXI 446 CCAGGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
518 CCAAGTCGCTGG
DdeI 49 CTGAG C^T[ACGT]AG
929 CTGAG
DpnII 675 GATC ^GATC
702 GATC
KspI 131 CCGCGG CCGC^GG
233 CCGCGG
MboI 675 GATC ^GATC
702 GATC
NaeI 481 GCCGGC GCC^GGC
909 GCCGGC
NdeII 675 GATC ^GATC
702 GATC
NgoMIV 481 GCCGGC G^CCGGC
909 GCCGGC
PvuII 251 CAGCTG CAG^CTG
763 CAGCTG
SacII 131 CCGCGG CCGC^GG
233 CCGCGG
Sau3AI 675 GATC ^GATC
702 GATC
SphI 191 GCATGC GCATG^C
380 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI Bsp1286I BspMI BsrFI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DsaI EaeI EagI
EclXI Eco52I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NlaIII NlaIV NotI NspI RsaI
Sau96I ScrFI SfoI StyI TseI