Restriction map for
pFN21AE2355
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AvrII BalI BamHI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI MaeI MamI
MfeI MluI MluNI MscI MunI
NcoI NdeI NheI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 1189 GACGTC GACGT^C
Acc65I 526 GGTACC G^GTACC
AccIII 301 TCCGGA T^CCGGA
AcsI 85 AAATTC [GA]^AATT[CT]
Alw44I 768 GTGCAC G^TGCAC
ApaLI 768 GTGCAC G^TGCAC
ApoI 85 AAATTC [GA]^AATT[CT]
Asp700I 1713 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 526 GGTACC G^GTACC
AviII 2402 TGCGCA TGC^GCA
BbrPI 562 CACGTG CAC^GTG
BsaAI 562 CACGTG [CT]AC^GT[GA]
BseAI 301 TCCGGA T^CCGGA
BsgI 508 CTGCAC GTGCAG(16/14)
BspEI 301 TCCGGA T^CCGGA
BsrDI 189 CATTGC GCAATG(2/0)
BssHII 735 GCGCGC G^CGCGC
BstYI 590 GGATCT [GA]^GATC[CT]
DraIII 511 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 2402 TGCGCA TGC^GCA
KpnI 526 GGTACC GGTAC^C
KspI 1505 CCGCGG CCGC^GG
MflI 590 GGATCT [GA]^GATC[CT]
MroI 301 TCCGGA T^CCGGA
MslI 9 CACAAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 2867 ACATGC [GA]CATG^[CT]
PmaCI 562 CACGTG CAC^GTG
PmlI 562 CACGTG CAC^GTG
PshAI 514 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2785 CAGCTG CAG^CTG
SacII 1505 CCGCGG CCGC^GG
SapI 823 GAAGAGC GCTCTTC(1/4)
StuI 48 AGGCCT AGG^CCT
XhoII 590 GGATCT [GA]^GATC[CT]
XmnI 1713 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 590 GGATC GGATC(4/5)
2477 GGATC
AlwNI 614 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2441 CAGGGCCTG
BbsI 1186 GAAGAC GAAGAC(2/6)
2533 GAAGAC
BlpI 670 GCTAAGC GC^T[ACGT]AGC
2265 GCTCAGC
Bpu1102I 670 GCTAAGC GC^T[ACGT]AGC
2265 GCTCAGC
BpuAI 1186 GAAGAC GAAGAC(2/6)
2533 GAAGAC
BsaI 540 GGTCTC GGTCTC(1/5)
1852 GGTCTC
BssSI 1986 CACGAG CACGAG(-5/-1)
1731 CTCGTG
CelII 670 GCTAAGC GC^T[ACGT]AGC
2265 GCTCAGC
DrdI 571 GACAGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2553 GACCCTGAAGTC
EcoNI 51 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
412 CCTCCTCCAGG
HindIII 28 AAGCTT A^AGCTT
2855 AAGCTT
MaeIII 2485 GTCAC ^GT[ACGT]AC
2864 GTGAC
NlaIII 456 CATG CATG^
2868 CATG
SacI 459 GAGCTC GAGCT^C
1018 GAGCTC
SfaNI 448 GATGC GCATC(5/9)
2781 GCATC
SfiI 1355 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1932 GGCCAGCCCGGCC
SmaI 982 CCCGGG CCC^GGG
2349 CCCGGG
SmlI 996 CTCAAG C^T[CT][GA]AG
2852 CTCAAG
Tsp45I 2485 GTCAC ^GT[GC]AC
2864 GTGAC
Tsp509I 86 AATT ^AATT
1434 AATT
XcmI 8 CCACAAGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1043 CCACTCTCCGCGTGG
XmaCI 982 CCCGGG C^CCGGG
2349 CCCGGG
XmaI 982 CCCGGG C^CCGGG
2349 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIV NotI NruI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfcI SfoI SgrAI
StyI TaqI TfiI Tru9I TseI
TspRI TthHB8I