Restriction map for
pFN21AE5266
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MflI MluI MroI MseI MslI
MspA1I MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 412 GTCTAC GT^[AC][GT]AC
AccII 361 CGCG CG^CG
AcyI 346 GGCGCC G[GA]^CG[CT]C
AlwI 183 GGATC GGATC(4/5)
AspHI 228 GTGCTC G[AT]GC[AT]^C
AvaI 257 CCCGGG C^[CT]CG[GA]G
BalI 393 TGGCCA TGG^CCA
BbeI 346 GGCGCC GGCGC^C
BbsI 471 GAAGAC GAAGAC(2/6)
BbvI 194 GCAGC GCAGC(8/12)
BcgI 373 CGACGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 87 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC
BpuAI 471 GAAGAC GAAGAC(2/6)
BsaHI 346 GGCGCC G[GA]^CG[CT]C
BsiHKAI 228 GTGCTC G[AT]GC[AT]^C
BsoBI 257 CCCGGG C^[CT]CG[GA]G
BspMI 514 GCAGGT ACCTGC(4/8)
BsrFI 123 GCCGGT [GA]^CCGG[CT]
BssHII 360 GCGCGC G^CGCGC
BstF5I 315 CATCC GGATG(2/0)
BstUI 361 CGCG CG^CG
CelII 87 GCTGAGC GC^T[ACGT]AGC
Cfr10I 123 GCCGGT [GA]^CCGG[CT]
DpnII 184 GATC ^GATC
EaeI 393 TGGCCA [CT]^GGCC[GA]
EcoT14I 179 CCAAGG C^C[AT][AT]GG
FauI 321 GCGGG CCCGC(4/6)
FokI 315 CATCC GGATG(9/13)
HgaI 374 GACGC GACGC(5/10)
Hin1I 346 GGCGCC G[GA]^CG[CT]C
KasI 346 GGCGCC G^GCGCC
MboI 184 GATC ^GATC
MboII 471 GAAGA GAAGA(8/7)
MluNI 393 TGGCCA TGG^CCA
MscI 393 TGGCCA TGG^CCA
MvnI 361 CGCG CG^CG
NarI 346 GGCGCC GG^CGCC
NdeII 184 GATC ^GATC
NlaIII 567 CATG CATG^
PflMI 385 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI 184 GATC ^GATC
SexAI 44 ACCTGGT A^CC[AT]GGT
SfaNI 144 GATGC GCATC(5/9)
SfcI 414 CTACAG C^T[GA][CT]AG
SfoI 346 GGCGCC GGC^GCC
SmaI 257 CCCGGG CCC^GGG
StyI 179 CCAAGG C^C[AT][AT]GG
TseI 194 GCAGC G^C[AT]GC
Van91I 385 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 257 CCCGGG C^CCGGG
XmaI 257 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AflIII 329 ACGTGT A^C[GA][CT]GT
352 ACACGT
AluI 213 AGCT AG^CT
449 AGCT
BanII 53 GGGCTC G[GA]GC[CT]^C
90 GAGCCC
BbrPI 277 CACGTG CAC^GTG
353 CACGTG
Fnu4HI 194 GCAGC GC^[ACGT]GC
344 GCGGC
HaeII 150 AGCGCC [GA]GCGC^[CT]
346 GGCGCC
ItaI 194 GCAGC GC^[ACGT]GC
344 GCGGC
MwoI 230 GCTCTTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
450 GCTGGTCCGGC
PmaCI 277 CACGTG CAC^GTG
353 CACGTG
PmlI 277 CACGTG CAC^GTG
353 CACGTG
TspRI 167 CAGTG CA[GC]TG[ACGT][ACGT]^
340 CAGTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AvaII BanI BcnI BmyI
BpmI BsaAI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII HaeIII HapII
HhaI HinP1I HinfI HpaII MaeII
MnlI MspI MvaI NciI NlaIV
PleI PpuMI Sau96I ScrFI