Restriction map for
pFN21AE9577
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MaeIII MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AccI 1363 GTCTAC GT^[AC][GT]AC
AflIII 854 ACACGT A^C[GA][CT]GT
AspHI 1468 GTGCTC G[AT]GC[AT]^C
BbrPI 622 CACGTG CAC^GTG
BcgI 972 CGAAGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 554 CTAG C^TAG
BglI 1132 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1107 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 27 CGGCCG CG[GA][CT]^CG
BsiHKAI 1468 GTGCTC G[AT]GC[AT]^C
BsmAI 251 GTCTC GTCTC(1/5)
BsrBI 131 GAGCGG CCGCTC(-3/-3)
BsrFI 104 ACCGGC [GA]^CCGG[CT]
Cfr10I 104 ACCGGC [GA]^CCGG[CT]
DraIII 622 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 27 CGGCCG C^GGCCG
EclXI 27 CGGCCG C^GGCCG
Eco52I 27 CGGCCG C^GGCCG
MaeI 554 CTAG C^TAG
MamI 1107 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PmaCI 622 CACGTG CAC^GTG
PmlI 622 CACGTG CAC^GTG
SexAI 572 ACCTGGT A^CC[AT]GGT
SfiI 1131 GGCCAACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 543 GATTC G^A[AT]TC
Tsp509I 685 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 1015 GGCGCC G[GA]^CG[CT]C
1171 GGCGCC
AlwNI 1123 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1420 CAGTGGCTG
Asp700I 147 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAGCTGTTC
AvaI 293 CCCGGG C^[CT]CG[GA]G
421 CCCGGG
BalI 1130 TGGCCA TGG^CCA
1313 TGGCCA
BbeI 1015 GGCGCC GGCGC^C
1171 GGCGCC
BbsI 402 GAAGAC GAAGAC(2/6)
865 GTCTTC
BpuAI 402 GAAGAC GAAGAC(2/6)
865 GTCTTC
BsaHI 1015 GGCGCC G[GA]^CG[CT]C
1171 GGCGCC
BsoBI 293 CCCGGG C^[CT]CG[GA]G
421 CCCGGG
BsrI 878 ACTGG ACTGG(1/-1)
654 CCAGT
BstYI 366 GGATCT [GA]^GATC[CT]
872 AGATCC
DsaI 30 CCGCGG C^C[GA][CT]GG
41 CCGCGG
EcoT14I 362 CCAAGG C^C[AT][AT]GG
893 CCTTGG
Hin1I 1015 GGCGCC G[GA]^CG[CT]C
1171 GGCGCC
HincII 528 GTCAAC GT[CT]^[GA]AC
1597 GTCAAC
HindII 528 GTCAAC GT[CT]^[GA]AC
1597 GTCAAC
KasI 1015 GGCGCC G^GCGCC
1171 GGCGCC
KspI 30 CCGCGG CCGC^GG
41 CCGCGG
MflI 366 GGATCT [GA]^GATC[CT]
872 AGATCC
MluNI 1130 TGGCCA TGG^CCA
1313 TGGCCA
MscI 1130 TGGCCA TGG^CCA
1313 TGGCCA
NarI 1015 GGCGCC GG^CGCC
1171 GGCGCC
PflMI 102 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1431 CCAGCTCTTGG
PleI 1567 GACTC GAGTC(4/5)
1156 GAGTC
PvuII 142 CAGCTG CAG^CTG
1516 CAGCTG
SacII 30 CCGCGG CCGC^GG
41 CCGCGG
SfoI 1015 GGCGCC GGC^GCC
1171 GGCGCC
SmaI 293 CCCGGG CCC^GGG
421 CCCGGG
StuI 975 AGGCCT AGG^CCT
1584 AGGCCT
StyI 362 CCAAGG C^C[AT][AT]GG
893 CCTTGG
Van91I 102 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1431 CCAGCTCTTGG
XhoII 366 GGATCT [GA]^GATC[CT]
872 AGATCC
XmaCI 293 CCCGGG C^CCGGG
421 CCCGGG
XmaI 293 CCCGGG C^CCGGG
421 CCCGGG
XmnI 147 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaAI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I
XcmI