Restriction map for
pFN21AE3424
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbsI BbvCI BcgI BclI
BfrI BglII BlnI BpuAI BsiWI
BsmI Bsp1407I BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I DraI DraIII DrdI
Eam1105I Eco81I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI MfeI MflI MluI MseI
MunI NdeI NheI NotI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 1115 TCCGGA T^CCGGA
AfaI 1502 GTAC GT^AC
AflIII 1498 ACGTGT A^C[GA][CT]GT
AlwI 336 GATCC GGATC(4/5)
Aor51HI 1040 AGCGCT AGC^GCT
AvaII 748 GGTCC G^G[AT]CC
AviII 1068 TGCGCA TGC^GCA
BbrPI 1497 CACGTG CAC^GTG
BciVI 1049 GTATCC GTATCC(6/5)
BglI 557 GCCTGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 771 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 771 GCTCAGC GC^T[ACGT]AGC
BsaAI 1497 CACGTG [CT]AC^GT[GA]
BsaBI 1362 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1115 TCCGGA [AT]^CCGG[AT]
BseAI 1115 TCCGGA T^CCGGA
BseRI 1479 CTCCTC GAGGAG(10/8)
BsiEI 104 CGGCCG CG[GA][CT]^CG
BsmBI 410 CGTCTC CGTCTC(1/5)
BspDI 23 ATCGAT AT^CGAT
BspEI 1115 TCCGGA T^CCGGA
BspHI 1421 TCATGA T^CATGA
BsrI 929 CCAGT ACTGG(1/-1)
BstEII 318 GGTCACC G^GT[ACGT]ACC
BstPI 318 GGTCACC G^GT[ACGT]ACC
CelII 771 GCTCAGC GC^T[ACGT]AGC
ClaI 23 ATCGAT AT^CGAT
CpoI 747 CGGTCCG CG^G[AT]CCG
EagI 104 CGGCCG C^GGCCG
EarI 1321 CTCTTC CTCTTC(1/4)
EclXI 104 CGGCCG C^GGCCG
Eco47III 1040 AGCGCT AGC^GCT
Eco52I 104 CGGCCG C^GGCCG
EcoNI 1160 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 318 GGTCACC G^GT[ACGT]ACC
EcoT14I 314 CCATGG C^C[AT][AT]GG
FseI 101 GGCCGGCC GGCCGG^CC
FspI 1068 TGCGCA TGC^GCA
Ksp632I 1321 CTCTTC CTCTTC(1/4)
MaeIII 319 GTCAC ^GT[ACGT]AC
MamI 1362 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1115 TCCGGA T^CCGGA
NcoI 314 CCATGG C^CATGG
NruI 933 TCGCGA TCG^CGA
PmaCI 1497 CACGTG CAC^GTG
PmlI 1497 CACGTG CAC^GTG
RcaI 1421 TCATGA T^CATGA
RsaI 1502 GTAC GT^AC
RsrII 747 CGGTCCG CG^G[AT]CCG
StyI 314 CCATGG C^C[AT][AT]GG
TfiI 1119 GAATC G^A[AT]TC
Tsp45I 319 GTCAC ^GT[GC]AC
Tsp509I 1270 AATT ^AATT
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
ApaI 159 GGGCCC GGGCC^C
237 GGGCCC
BalI 185 TGGCCA TGG^CCA
971 TGGCCA
BbeI 217 GGCGCC GGCGC^C
1433 GGCGCC
BfaI 43 CTAG C^TAG
706 CTAG
BsaI 1156 GAGACC GGTCTC(1/5)
1088 GGTCTC
BsgI 379 CTGCAC GTGCAG(16/14)
883 CTGCAC
BsrBI 457 CCGCTC CCGCTC(-3/-3)
937 GAGCGG
HgaI 787 GACGC GACGC(5/10)
301 GCGTC
KasI 217 GGCGCC G^GCGCC
1433 GGCGCC
MaeI 43 CTAG C^TAG
706 CTAG
MluNI 185 TGGCCA TGG^CCA
971 TGGCCA
MscI 185 TGGCCA TGG^CCA
971 TGGCCA
NaeI 102 GCCGGC GCC^GGC
106 GCCGGC
NarI 217 GGCGCC GG^CGCC
1433 GGCGCC
NgoMIV 102 GCCGGC G^CCGGC
106 GCCGGC
NspI 395 GCATGT [GA]CATG^[CT]
1205 GCATGT
PflMI 264 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1380 CCAGCAGCTGG
PvuII 985 CAGCTG CAG^CTG
1384 CAGCTG
SacI 364 GAGCTC GAGCT^C
1396 GAGCTC
SfcI 630 CTACAG C^T[GA][CT]AG
1044 CTACAG
SfoI 217 GGCGCC GGC^GCC
1433 GGCGCC
SmaI 162 CCCGGG CCC^GGG
891 CCCGGG
Van91I 264 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1380 CCAGCAGCTGG
XmaCI 162 CCCGGG C^CCGGG
891 CCCGGG
XmaI 162 CCCGGG C^CCGGG
891 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaI BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KspI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
SacII Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I