Restriction map for
pFN21AE6366
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbvCI BcgI
BciVI BcnI BfrI BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AccI 754 GTCTAC GT^[AC][GT]AC
AciI 16 CCGC CCGC(-3/-1)
AcyI 946 GGCGTC G[GA]^CG[CT]C
AflIII 489 ACATGT A^C[GA][CT]GT
Aor51HI 914 AGCGCT AGC^GCT
AvaI 923 CTCGGG C^[CT]CG[GA]G
AvaII 376 GGACC G^G[AT]CC
BanII 673 GAGCCC G[GA]GC[CT]^C
BbrPI 435 CACGTG CAC^GTG
BbsI 396 GTCTTC GAAGAC(2/6)
BclI 503 TGATCA T^GATCA
BglII 76 AGATCT A^GATCT
BmyI 673 GAGCCC G[AGT]GC[ACT]^C
BpuAI 396 GTCTTC GAAGAC(2/6)
BsaAI 435 CACGTG [CT]AC^GT[GA]
BsaHI 946 GGCGTC G[GA]^CG[CT]C
BsaJI 146 CCTGGG C^C[ACGT][ACGT]GG
BsgI 988 CTGCAC GTGCAG(16/14)
BsmAI 189 GTCTC GTCTC(1/5)
BsmFI 274 GGGAC GGGAC(10/14)
BsmI 21 GAATGC GAATGC(1/-1)
BsoBI 923 CTCGGG C^[CT]CG[GA]G
Bsp1286I 673 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 489 ACATGT A^CATGT
BsrDI 358 GCAATG GCAATG(2/0)
BsrFI 943 GCCGGC [GA]^CCGG[CT]
BstYI 76 AGATCT [GA]^GATC[CT]
CfoI 915 GCGC GCG^C
Cfr10I 943 GCCGGC [GA]^CCGG[CT]
Cfr13I 376 GGACC G^G[ACGT]CC
DraI 514 TTTAAA TTT^AAA
EarI 718 GAAGAG CTCTTC(1/4)
Eco47III 914 AGCGCT AGC^GCT
EcoT22I 1157 ATGCAT ATGCA^T
FbaI 503 TGATCA T^GATCA
HaeII 914 AGCGCT [GA]GCGC^[CT]
HapII 944 CCGG C^CGG
HgaI 947 GCGTC GACGC(5/10)
HhaI 915 GCGC GCG^C
Hin1I 946 GGCGTC G[GA]^CG[CT]C
HinP1I 915 GCGC G^CGC
HpaII 944 CCGG C^CGG
Ksp632I 718 GAAGAG CTCTTC(1/4)
MaeII 436 ACGT A^CGT
MfeI 1013 CAATTG C^AATTG
MflI 76 AGATCT [GA]^GATC[CT]
MspA1I 16 CCGCTG C[AC]G^C[GT]G
MspI 944 CCGG C^CGG
MunI 1013 CAATTG C^AATTG
NaeI 943 GCCGGC GCC^GGC
NgoMIV 943 GCCGGC G^CCGGC
NlaIV 12 GGTTCC GG[ACGT]^[ACGT]CC
NsiI 1157 ATGCAT ATGCA^T
NspI 489 ACATGT [GA]CATG^[CT]
PleI 664 GACTC GAGTC(4/5)
PmaCI 435 CACGTG CAC^GTG
PmlI 435 CACGTG CAC^GTG
PstI 701 CTGCAG CTGCA^G
Sau96I 376 GGACC G^G[ACGT]CC
ScaI 1054 AGTACT AGT^ACT
SfcI 701 CTGCAG C^T[GA][CT]AG
StuI 462 AGGCCT AGG^CCT
TaqI 639 TCGA T^CGA
TspRI 263 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 639 TCGA T^CGA
XhoII 76 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 238 AAATTT [GA]^AATT[CT]
1199 GAATTT
AfaI 769 GTAC GT^AC
1055 GTAC
ApoI 238 AAATTT [GA]^AATT[CT]
1199 GAATTT
BbvI 696 GCAGC GCAGC(8/12)
1021 GCTGC
BglI 952 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1168 GCCATGCTGGC
BseRI 616 GAGGAG GAGGAG(10/8)
928 GAGGAG
BsrI 264 ACTGG ACTGG(1/-1)
822 CCAGT
BstAPI 1012 GCAATTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1246 GCACTTACTGC
EaeI 266 TGGCCG [CT]^GGCC[GA]
819 CGGCCA
Fnu4HI 696 GCAGC GC^[ACGT]GC
1021 GCTGC
ItaI 696 GCAGC GC^[ACGT]GC
1021 GCTGC
RsaI 769 GTAC GT^AC
1055 GTAC
TseI 696 GCAGC G^C[AT]GC
1021 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI BfaI BspHI BssKI BstF5I
BstNI Cac8I DdeI DpnII EcoRII
FokI HaeIII HinfI HphI MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
RcaI Sau3AI ScrFI SfaNI TfiI
Tru9I Tsp45I Tsp509I