Restriction map for
pFN21AE5467
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MluI MluNI MroI MscI MseI
MspA1I MvnI MwoI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 73 AAATTC [GA]^AATT[CT]
AfaI 550 GTAC GT^AC
ApoI 73 AAATTC [GA]^AATT[CT]
AspHI 64 GAGCTC G[AT]GC[AT]^C
AvaI 684 CTCGGG C^[CT]CG[GA]G
AvaII 677 GGTCC G^G[AT]CC
BanII 64 GAGCTC G[GA]GC[CT]^C
BbvCI 672 GCTGAGG CCTCAGC(-5/-2)
BglII 299 AGATCT A^GATCT
BmyI 64 GAGCTC G[AGT]GC[ACT]^C
BsaI 262 GAGACC GGTCTC(1/5)
BsaWI 389 TCCGGT [AT]^CCGG[AT]
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsoBI 684 CTCGGG C^[CT]CG[GA]G
Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C
BspMI 715 ACCTGC ACCTGC(4/8)
BsrFI 158 GCCGGC [GA]^CCGG[CT]
BsrI 477 ACTGG ACTGG(1/-1)
BstF5I 660 CATCC GGATG(2/0)
BstXI 110 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 299 AGATCT [GA]^GATC[CT]
Cfr10I 158 GCCGGC [GA]^CCGG[CT]
DraII 676 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 676 AGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 660 CATCC GGATG(9/13)
MfeI 415 CAATTG C^AATTG
MflI 299 AGATCT [GA]^GATC[CT]
MunI 415 CAATTG C^AATTG
NaeI 158 GCCGGC GCC^GGC
NgoMIV 158 GCCGGC G^CCGGC
PflMI 312 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 676 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 550 GTAC GT^AC
SacI 64 GAGCTC GAGCT^C
ScaI 549 AGTACT AGT^ACT
Van91I 312 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 467 CCATTGTCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 299 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
440 CCGC
BpmI 67 CTCCAG CTGGAG(16/14)
583 CTGGAG
BsaJI 282 CCAGGG C^C[ACGT][ACGT]GG
443 CCTGGG
BseRI 634 CTCCTC GAGGAG(10/8)
894 GAGGAG
BsgI 564 CTGCAC GTGCAG(16/14)
717 CTGCAC
BsmAI 262 GAGAC GTCTC(1/5)
555 GAGAC
BsmFI 328 GGGAC GGGAC(10/14)
678 GTCCC
Cac8I 158 GCCGGC GC[ACGT]^[ACGT]GC
562 GCCTGC
HapII 159 CCGG C^CGG
390 CCGG
HpaII 159 CCGG C^CGG
390 CCGG
MspI 159 CCGG C^CGG
390 CCGG
SfaNI 240 GATGC GCATC(5/9)
669 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI BbvI BfaI BsiYI BslI
BspHI BssKI BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MslI MvaI NdeII NlaIII
NlaIV RcaI Sau3AI Sau96I ScrFI
TseI Tsp45I Tsp509I TspRI