Restriction map for
pFN21AE3223
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbvCI BciVI BclI BfrI
BglI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI Tru9I
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 112 CGCG CG^CG
AcsI 1303 GAATTC [GA]^AATT[CT]
AflIII 617 ACGTGT A^C[GA][CT]GT
ApaI 1008 GGGCCC GGGCC^C
ApoI 1303 GAATTC [GA]^AATT[CT]
Asp700I 1050 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1066 GAGCTC G[AT]GC[AT]^C
AvaI 763 CTCGAG C^[CT]CG[GA]G
BamHI 909 GGATCC G^GATCC
BanI 1487 GGCGCC G^G[CT][GA]CC
BbeI 1487 GGCGCC GGCGC^C
BbrPI 16 CACGTG CAC^GTG
BfaI 344 CTAG C^TAG
BglII 1082 AGATCT A^GATCT
BlpI 589 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 589 GCTCAGC GC^T[ACGT]AGC
BsaAI 16 CACGTG [CT]AC^GT[GA]
BsaI 1042 GAGACC GGTCTC(1/5)
BsiHKAI 1066 GAGCTC G[AT]GC[AT]^C
BsmBI 938 GAGACG CGTCTC(1/5)
BsoBI 763 CTCGAG C^[CT]CG[GA]G
BstEII 1194 GGTTACC G^GT[ACGT]ACC
BstPI 1194 GGTTACC G^GT[ACGT]ACC
BstUI 112 CGCG CG^CG
CelII 589 GCTCAGC GC^T[ACGT]AGC
CfoI 1488 GCGC GCG^C
DsaI 1199 CCATGG C^C[GA][CT]GG
EaeI 258 TGGCCG [CT]^GGCC[GA]
EcoO65I 1194 GGTTACC G^GT[ACGT]ACC
EcoRI 1303 GAATTC G^AATTC
HaeII 1487 GGCGCC [GA]GCGC^[CT]
HgaI 628 GCGTC GACGC(5/10)
HhaI 1488 GCGC GCG^C
HinP1I 1488 GCGC G^CGC
HincII 364 GTCAAC GT[CT]^[GA]AC
HindII 364 GTCAAC GT[CT]^[GA]AC
HindIII 1231 AAGCTT A^AGCTT
KasI 1487 GGCGCC G^GCGCC
MaeI 344 CTAG C^TAG
MslI 612 CATTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 112 CGCG CG^CG
NarI 1487 GGCGCC GG^CGCC
NcoI 1199 CCATGG C^CATGG
NspI 140 ACATGC [GA]CATG^[CT]
PaeR7I 763 CTCGAG C^TCGAG
PflMI 1332 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 16 CACGTG CAC^GTG
PmlI 16 CACGTG CAC^GTG
SacI 1066 GAGCTC GAGCT^C
SbfI 1167 CCTGCAGG CCTGCA^GG
SfoI 1487 GGCGCC GGC^GCC
Sse8387I 1167 CCTGCAGG CCTGCA^GG
SspI 250 AATATT AAT^ATT
StuI 236 AGGCCT AGG^CCT
Tsp45I 1297 GTGAC ^GT[GC]AC
Tsp509I 1304 AATT ^AATT
Van91I 1332 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 763 CTCGAG C^TCGAG
XmnI 1050 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 627 GGCGTC G[GA]^CG[CT]C
1487 GGCGCC
BbsI 1431 GAAGAC GAAGAC(2/6)
1241 GTCTTC
BcgI 942 CGATGCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
41 GCAAGCTGCTCG
BcnI 891 CCCGG CC^[GC]GG
1046 CCCGG
BpuAI 1431 GAAGAC GAAGAC(2/6)
1241 GTCTTC
BsaHI 627 GGCGTC G[GA]^CG[CT]C
1487 GGCGCC
BsgI 688 GTGCAG GTGCAG(16/14)
1363 GTGCAG
BsmAI 938 GAGAC GTCTC(1/5)
1042 GAGAC
EarI 267 CTCTTC CTCTTC(1/4)
154 GAAGAG
EcoNI 543 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1274 CCTCACCAAGG
HapII 892 CCGG C^CGG
1047 CCGG
Hin1I 627 GGCGTC G[GA]^CG[CT]C
1487 GGCGCC
HpaII 892 CCGG C^CGG
1047 CCGG
Ksp632I 267 CTCTTC CTCTTC(1/4)
154 GAAGAG
MaeII 17 ACGT A^CGT
617 ACGT
MaeIII 1195 GTTAC ^GT[ACGT]AC
1297 GTGAC
MspI 892 CCGG C^CGG
1047 CCGG
NciI 891 CCCGG CC^[GC]GG
1046 CCCGG
PpuMI 178 GGGTCCT [GA]G^G[AT]CC[CT]
551 AGGACCT
XcmI 165 CCAGCTGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1181 CCAAAGAGAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI MboI MboII
MflI MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI TspRI TthHB8I XhoII