Restriction map for
pFN21AE1908
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AhdI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsiEI
BsiWI BsmI BspDI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 286 CGCG CG^CG
AccIII 1121 TCCGGA T^CCGGA
AgeI 1136 ACCGGT A^CCGGT
Alw44I 340 GTGCAC G^TGCAC
ApaI 819 GGGCCC GGGCC^C
ApaLI 340 GTGCAC G^TGCAC
BbsI 1027 GTCTTC GAAGAC(2/6)
BclI 842 TGATCA T^GATCA
BfaI 187 CTAG C^TAG
BglI 1165 GCCCGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 73 CTCCAG CTGGAG(16/14)
BpuAI 1027 GTCTTC GAAGAC(2/6)
BsaBI 687 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1121 TCCGGA T^CCGGA
BseRI 322 GAGGAG GAGGAG(10/8)
BsmFI 99 GTCCC GGGAC(10/14)
Bsp1407I 797 TGTACA T^GTACA
BspEI 1121 TCCGGA T^CCGGA
BspHI 881 TCATGA T^CATGA
BsrGI 797 TGTACA T^GTACA
BsrI 464 ACTGG ACTGG(1/-1)
BstAPI 437 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 286 CGCG CG^CG
BstYI 311 AGATCC [GA]^GATC[CT]
DsaI 140 CCGTGG C^C[GA][CT]GG
EarI 1125 GAAGAG CTCTTC(1/4)
EcoNI 315 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 842 TGATCA T^GATCA
HinfI 1054 GAATC G^A[ACGT]TC
Ksp632I 1125 GAAGAG CTCTTC(1/4)
MaeI 187 CTAG C^TAG
MamI 687 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 311 AGATCC [GA]^GATC[CT]
MroI 1121 TCCGGA T^CCGGA
MspA1I 273 CAGCTG C[AC]G^C[GT]G
MvnI 286 CGCG CG^CG
NspI 980 ACATGC [GA]CATG^[CT]
PinAI 1136 ACCGGT A^CCGGT
PstI 124 CTGCAG CTGCA^G
PvuII 273 CAGCTG CAG^CTG
RcaI 881 TCATGA T^CATGA
SexAI 502 ACCTGGT A^CC[AT]GGT
SfcI 124 CTGCAG C^T[GA][CT]AG
SgrAI 1135 CACCGGTG C[GA]^CCGG[CT]G
SspBI 797 TGTACA T^GTACA
StuI 787 AGGCCT AGG^CCT
TaqI 1156 TCGA T^CGA
TfiI 1054 GAATC G^A[AT]TC
TthHB8I 1156 TCGA T^CGA
XcmI 500 CCACCTGGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 311 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 340 GTGCAC G[AT]GC[AT]^C
652 GTGCTC
AviII 491 TGCGCA TGC^GCA
677 TGCGCA
BalI 104 TGGCCA TGG^CCA
263 TGGCCA
BanII 528 GAGCCC G[GA]GC[CT]^C
819 GGGCCC
BsaI 913 GAGACC GGTCTC(1/5)
1014 GGTCTC
BsgI 637 CTGCAC GTGCAG(16/14)
561 GTGCAG
BsiHKAI 340 GTGCAC G[AT]GC[AT]^C
652 GTGCTC
BsmBI 22 CGTCTC CGTCTC(1/5)
60 CGTCTC
BsrFI 671 ACCGGC [GA]^CCGG[CT]
1136 ACCGGT
BstXI 722 CCATCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
824 CCATGATCCTGG
CfoI 492 GCGC GCG^C
678 GCGC
Cfr10I 671 ACCGGC [GA]^CCGG[CT]
1136 ACCGGT
EaeI 104 TGGCCA [CT]^GGCC[GA]
263 TGGCCA
FspI 491 TGCGCA TGC^GCA
677 TGCGCA
HhaI 492 GCGC GCG^C
678 GCGC
HinP1I 492 GCGC G^CGC
678 GCGC
MaeII 551 ACGT A^CGT
907 ACGT
MaeIII 571 GTGAC ^GT[ACGT]AC
598 GTGAC
MluNI 104 TGGCCA TGG^CCA
263 TGGCCA
MscI 104 TGGCCA TGG^CCA
263 TGGCCA
SmaI 1000 CCCGGG CCC^GGG
1019 CCCGGG
Tsp45I 571 GTGAC ^GT[GC]AC
598 GTGAC
Tsp509I 292 AATT ^AATT
847 AATT
XmaCI 1000 CCCGGG C^CCGGG
1019 CCCGGG
XmaI 1000 CCCGGG C^CCGGG
1019 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BbvI BcnI BmyI
BsaJI BsaWI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI