Restriction map for
pFN21AE2242
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AviII BalI BbeI
BbrPI BbsI BbvCI BciVI BfrI
BglI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KspI MaeII MamI
MluI MluNI MroI MscI MspA1I
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 895 GGTACC G^GTACC
AccII 72 CGCG CG^CG
AcyI 1014 GACGCC G[GA]^CG[CT]C
Alw44I 657 GTGCAC G^TGCAC
AlwNI 796 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 727 GGGCCC GGGCC^C
ApaLI 657 GTGCAC G^TGCAC
Asp718I 895 GGTACC G^GTACC
AspI 76 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 724 CTCGGG C^[CT]CG[GA]G
AvrII 969 CCTAGG C^CTAGG
BamHI 540 GGATCC G^GATCC
BcgI 335 GCAGCCGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 803 TGATCA T^GATCA
BlnI 969 CCTAGG C^CTAGG
BpmI 304 CTGGAG CTGGAG(16/14)
BsaHI 1014 GACGCC G[GA]^CG[CT]C
BsaI 580 GAGACC GGTCTC(1/5)
BsiWI 708 CGTACG C^GTACG
BsmAI 580 GAGAC GTCTC(1/5)
BsoBI 724 CTCGGG C^[CT]CG[GA]G
BstF5I 883 GGATG GGATG(2/0)
BstUI 72 CGCG CG^CG
CfoI 672 GCGC GCG^C
EcoT14I 969 CCTAGG C^C[AT][AT]GG
FauI 287 CCCGC CCCGC(4/6)
FbaI 803 TGATCA T^GATCA
FokI 883 GGATG GGATG(9/13)
HgaI 1014 GACGC GACGC(5/10)
HhaI 672 GCGC GCG^C
Hin1I 1014 GACGCC G[GA]^CG[CT]C
HinP1I 672 GCGC G^CGC
KpnI 895 GGTACC GGTAC^C
MfeI 261 CAATTG C^AATTG
MunI 261 CAATTG C^AATTG
MvnI 72 CGCG CG^CG
NaeI 809 GCCGGC GCC^GGC
NgoMIV 809 GCCGGC G^CCGGC
PflFI 76 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 910 GAGTC GAGTC(4/5)
PpuMI 664 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 756 GAGCTC GAGCT^C
SapI 237 GAAGAGC GCTCTTC(1/4)
SexAI 77 ACCTGGT A^CC[AT]GGT
SfaNI 1022 GCATC GCATC(5/9)
StyI 969 CCTAGG C^C[AT][AT]GG
Tth111I 76 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 657 GTGCAC G[AT]GC[AT]^C
756 GAGCTC
BanI 895 GGTACC G^G[CT][GA]CC
1005 GGCACC
BanII 727 GGGCCC G[GA]GC[CT]^C
756 GAGCTC
BcnI 120 CCGGG CC^[GC]GG
199 CCGGG
BsiHKAI 657 GTGCAC G[AT]GC[AT]^C
756 GAGCTC
BsrDI 276 CATTGC GCAATG(2/0)
621 GCAATG
BsrFI 809 GCCGGC [GA]^CCGG[CT]
1002 ACCGGC
BstYI 496 AGATCC [GA]^GATC[CT]
540 GGATCC
Cac8I 608 GCCTGC GC[ACGT]^[ACGT]GC
809 GCCGGC
Cfr10I 809 GCCGGC [GA]^CCGG[CT]
1002 ACCGGC
EarI 237 GAAGAG CTCTTC(1/4)
459 GAAGAG
EcoNI 78 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
741 CCTTCCAGAGG
HphI 630 GGTGA GGTGA(8/7)
841 TCACC
Ksp632I 237 GAAGAG CTCTTC(1/4)
459 GAAGAG
MaeIII 392 GTGAC ^GT[ACGT]AC
912 GTCAC
MflI 496 AGATCC [GA]^GATC[CT]
540 GGATCC
MseI 508 TTAA T^TAA
548 TTAA
MslI 514 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
685 CACACAGATG
NciI 120 CCGGG CC^[GC]GG
199 CCGGG
NlaIII 171 CATG CATG^
783 CATG
Tru9I 508 TTAA T^TAA
548 TTAA
Tsp45I 392 GTGAC ^GT[GC]AC
912 GTCAC
TspRI 232 CAGTG CA[GC]TG[ACGT][ACGT]^
817 CAGTG
XhoII 496 AGATCC [GA]^GATC[CT]
540 GGATCC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HinfI HpaII ItaI MaeI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI TaqI TfiI TseI
Tsp509I TthHB8I