Restriction map for
pFN21AE4022
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MboI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MspA1I MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 575 GGTACC G^GTACC
AccII 175 CGCG CG^CG
Asp718I 575 GGTACC G^GTACC
AspHI 267 GAGCAC G[AT]GC[AT]^C
BanI 575 GGTACC G^G[CT][GA]CC
BsiHKAI 267 GAGCAC G[AT]GC[AT]^C
BspHI 389 TCATGA T^CATGA
BsrI 251 ACTGG ACTGG(1/-1)
BssSI 520 CTCGTG CACGAG(-5/-1)
BstAPI 234 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 175 CGCG CG^CG
CfoI 166 GCGC GCG^C
DdeI 62 CTAAG C^T[ACGT]AG
HaeII 165 GGCGCT [GA]GCGC^[CT]
HhaI 166 GCGC GCG^C
HinP1I 166 GCGC G^CGC
HphI 192 GGTGA GGTGA(8/7)
KpnI 575 GGTACC GGTAC^C
MaeII 219 ACGT A^CGT
MvnI 175 CGCG CG^CG
PflMI 224 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 491 GAGTC GAGTC(4/5)
PstI 457 CTGCAG CTGCA^G
RcaI 389 TCATGA T^CATGA
SfcI 457 CTGCAG C^T[GA][CT]AG
SmaI 556 CCCGGG CCC^GGG
Tsp509I 506 AATT ^AATT
Van91I 224 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 556 CCCGGG C^CCGGG
XmaI 556 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 221 GTAC GT^AC
576 GTAC
AvaII 15 GGACC G^G[AT]CC
410 GGTCC
BfaI 343 CTAG C^TAG
439 CTAG
BpmI 414 CTCCAG CTGGAG(16/14)
187 CTGGAG
BsmAI 282 GTCTC GTCTC(1/5)
606 GTCTC
BsmFI 75 GGGAC GGGAC(10/14)
363 GTCCC
BspMI 26 ACCTGC ACCTGC(4/8)
114 GCAGGT
Cac8I 30 GCCTGC GC[ACGT]^[ACGT]GC
243 GCTTGC
DraII 14 AGGACCC [GA]G^G[ACGT]CC[CT]
409 AGGTCCT
EcoO109I 14 AGGACCC [GA]G^G[ACGT]CC[CT]
409 AGGTCCT
FauI 173 CCCGC CCCGC(4/6)
318 GCGGG
HaeIII 322 GGCC GG^CC
525 GGCC
HgaI 203 GACGC GACGC(5/10)
176 GCGTC
MaeI 343 CTAG C^TAG
439 CTAG
MaeIII 333 GTCAC ^GT[ACGT]AC
512 GTGAC
MslI 516 CATCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
593 CACAGGCATG
PpuMI 14 AGGACCC [GA]G^G[AT]CC[CT]
409 AGGTCCT
RsaI 221 GTAC GT^AC
576 GTAC
SfaNI 240 GATGC GCATC(5/9)
431 GCATC
TfiI 419 GAATC G^A[AT]TC
485 GATTC
Tsp45I 333 GTCAC ^GT[GC]AC
512 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI Cfr13I
EcoRII Fnu4HI FokI HapII HinfI
HpaII ItaI MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI TseI TspRI