Restriction map for
pFN21AE5813
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AviII BbeI
BclI BfrI BglII BsaBI BseAI
BsiEI BsmI BspDI BspEI BspLU11I
BsrDI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
FspI HincII HindII HpaI KasI
KspI MamI MroI NarI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 758 GGTACC G^GTACC
AccII 337 CGCG CG^CG
AcyI 964 GGCGTC G[GA]^CG[CT]C
AflIII 336 ACGCGT A^C[GA][CT]GT
AocI 2370 CCTCAGG CC^T[ACGT]AGG
ApaI 821 GGGCCC GGGCC^C
Asp718I 758 GGTACC G^GTACC
AspI 1927 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2268 CCTAGG C^CTAGG
BamHI 2085 GGATCC G^GATCC
BbrPI 193 CACGTG CAC^GTG
BbvCI 2045 CCTCAGC CCTCAGC(-5/-2)
BcgI 1695 GCAGTACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1248 GTATCC GTATCC(6/5)
BglI 1735 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2268 CCTAGG C^CTAGG
BlpI 1690 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1690 GCTGAGC GC^T[ACGT]AGC
BsaAI 193 CACGTG [CT]AC^GT[GA]
BsaHI 964 GGCGTC G[GA]^CG[CT]C
BsiWI 339 CGTACG C^GTACG
BsmBI 595 GAGACG CGTCTC(1/5)
BspHI 21 TCATGA T^CATGA
BsrBI 853 CCGCTC CCGCTC(-3/-3)
BstBI 1615 TTCGAA TT^CGAA
BstUI 337 CGCG CG^CG
BstYI 2085 GGATCC [GA]^GATC[CT]
Bsu36I 2370 CCTCAGG CC^T[ACGT]AGG
CelII 1690 GCTGAGC GC^T[ACGT]AGC
DraIII 1457 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2370 CCTCAGG CC^T[ACGT]AGG
EcoRI 1951 GAATTC G^AATTC
EcoT22I 2700 ATGCAT ATGCA^T
HaeII 2645 AGCGCC [GA]GCGC^[CT]
HgaI 965 GCGTC GACGC(5/10)
Hin1I 964 GGCGTC G[GA]^CG[CT]C
HindIII 1511 AAGCTT A^AGCTT
KpnI 758 GGTACC GGTAC^C
MfeI 1935 CAATTG C^AATTG
MflI 2085 GGATCC [GA]^GATC[CT]
MluI 336 ACGCGT A^CGCGT
MunI 1935 CAATTG C^AATTG
MvnI 337 CGCG CG^CG
NaeI 1004 GCCGGC GCC^GGC
NdeI 1292 CATATG CA^TATG
NgoMIV 1004 GCCGGC G^CCGGC
NsiI 2700 ATGCAT ATGCA^T
NspV 1615 TTCGAA TT^CGAA
PflFI 1927 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1410 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 193 CACGTG CAC^GTG
PmlI 193 CACGTG CAC^GTG
PpuMI 2513 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 21 TCATGA T^CATGA
SacI 1042 GAGCTC GAGCT^C
SfuI 1615 TTCGAA TT^CGAA
SmaI 811 CCCGGG CCC^GGG
StuI 450 AGGCCT AGG^CCT
Tth111I 1927 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1410 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 303 TCTAGA T^CTAGA
XcmI 2582 CCAAGCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2085 GGATCC [GA]^GATC[CT]
XmaCI 811 CCCGGG C^CCGGG
XmaI 811 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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Alw44I 196 GTGCAC G^TGCAC
514 GTGCAC
ApaLI 196 GTGCAC G^TGCAC
514 GTGCAC
AvaI 811 CCCGGG C^[CT]CG[GA]G
2245 CCCGAG
BsaI 1021 GAGACC GGTCTC(1/5)
2541 GGTCTC
BsaWI 675 ACCGGA [AT]^CCGG[AT]
761 ACCGGA
BsoBI 811 CCCGGG C^[CT]CG[GA]G
2245 CCCGAG
Bsp1407I 947 TGTACA T^GTACA
2651 TGTACA
BspMI 578 ACCTGC ACCTGC(4/8)
2708 ACCTGC
BsrGI 947 TGTACA T^GTACA
2651 TGTACA
CfoI 901 GCGC GCG^C
2646 GCGC
EcoNI 2678 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2766 CCTACAAGAGG
FauI 824 CCCGC CCCGC(4/6)
1882 CCCGC
HhaI 901 GCGC GCG^C
2646 GCGC
HinP1I 901 GCGC G^CGC
2646 GCGC
MseI 431 TTAA T^TAA
1388 TTAA
NcoI 1133 CCATGG C^CATGG
1639 CCATGG
SexAI 539 ACCTGGT A^CC[AT]GGT
1661 ACCTGGT
SspBI 947 TGTACA T^GTACA
2651 TGTACA
Tru9I 431 TTAA T^TAA
1388 TTAA
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I