Restriction map for
pFN21AE6456
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BsaAI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1582 GTCTAC GT^[AC][GT]AC
AcsI 261 GAATTC [GA]^AATT[CT]
AcyI 1034 GGCGCC G[GA]^CG[CT]C
AfaI 1391 GTAC GT^AC
AflIII 556 ACATGT A^C[GA][CT]GT
AhdI 900 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 261 GAATTC [GA]^AATT[CT]
AspEI 900 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 920 GAGCAC G[AT]GC[AT]^C
AspI 1555 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1321 CCTAGG C^CTAGG
BalI 1631 TGGCCA TGG^CCA
BamHI 889 GGATCC G^GATCC
BbeI 1034 GGCGCC GGCGC^C
BfaI 1322 CTAG C^TAG
BglI 1516 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1321 CCTAGG C^CTAGG
BpmI 1291 CTCCAG CTGGAG(16/14)
BsaHI 1034 GGCGCC G[GA]^CG[CT]C
BsaI 847 GAGACC GGTCTC(1/5)
BsaWI 1077 ACCGGA [AT]^CCGG[AT]
BsiHKAI 920 GAGCAC G[AT]GC[AT]^C
BsmBI 538 CGTCTC CGTCTC(1/5)
BspLU11I 556 ACATGT A^CATGT
BsrBI 68 CCGCTC CCGCTC(-3/-3)
BstYI 889 GGATCC [GA]^GATC[CT]
DsaI 931 CCATGG C^C[GA][CT]GG
Eam1105I 900 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 319 GAAGAG CTCTTC(1/4)
EcoNI 48 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 261 GAATTC G^AATTC
HaeII 1034 GGCGCC [GA]GCGC^[CT]
Hin1I 1034 GGCGCC G[GA]^CG[CT]C
KasI 1034 GGCGCC G^GCGCC
Ksp632I 319 GAAGAG CTCTTC(1/4)
MaeI 1322 CTAG C^TAG
MflI 889 GGATCC [GA]^GATC[CT]
MluNI 1631 TGGCCA TGG^CCA
MscI 1631 TGGCCA TGG^CCA
NarI 1034 GGCGCC GG^CGCC
NcoI 931 CCATGG C^CATGG
PflFI 1555 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 479 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 302 GACTC GAGTC(4/5)
PshAI 590 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1391 GTAC GT^AC
SfoI 1034 GGCGCC GGC^GCC
SphI 1341 GCATGC GCATG^C
TfiI 667 GATTC G^A[AT]TC
Tsp509I 262 AATT ^AATT
Tth111I 1555 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 479 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 889 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 33 CGCG CG^CG
1536 CGCG
AocI 112 CCTCAGG CC^T[ACGT]AGG
1129 CCTGAGG
BbsI 59 GTCTTC GAAGAC(2/6)
1528 GTCTTC
BlpI 70 GCTCAGC GC^T[ACGT]AGC
1612 GCTGAGC
Bpu1102I 70 GCTCAGC GC^T[ACGT]AGC
1612 GCTGAGC
BpuAI 59 GTCTTC GAAGAC(2/6)
1528 GTCTTC
BsmAI 847 GAGAC GTCTC(1/5)
539 GTCTC
BsrFI 1334 GCCGGC [GA]^CCGG[CT]
1521 GCCGGC
BstUI 33 CGCG CG^CG
1536 CGCG
Bsu36I 112 CCTCAGG CC^T[ACGT]AGG
1129 CCTGAGG
CelII 70 GCTCAGC GC^T[ACGT]AGC
1612 GCTGAGC
Cfr10I 1334 GCCGGC [GA]^CCGG[CT]
1521 GCCGGC
DpnII 490 GATC ^GATC
890 GATC
DrdI 205 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1573 GACTGGCAGGTC
EaeI 1475 TGGCCG [CT]^GGCC[GA]
1631 TGGCCA
Eco81I 112 CCTCAGG CC^T[ACGT]AGG
1129 CCTGAGG
HgaI 757 GACGC GACGC(5/10)
537 GCGTC
HinfI 302 GACTC G^A[ACGT]TC
667 GATTC
MboI 490 GATC ^GATC
890 GATC
MvnI 33 CGCG CG^CG
1536 CGCG
NaeI 1334 GCCGGC GCC^GGC
1521 GCCGGC
NdeII 490 GATC ^GATC
890 GATC
NgoMIV 1334 GCCGGC G^CCGGC
1521 GCCGGC
NspI 556 ACATGT [GA]CATG^[CT]
1341 GCATGC
PaeR7I 691 CTCGAG C^TCGAG
809 CTCGAG
Sau3AI 490 GATC ^GATC
890 GATC
TaqI 692 TCGA T^CGA
810 TCGA
Tsp45I 1174 GTGAC ^GT[GC]AC
1306 GTGAC
TthHB8I 692 TCGA T^CGA
810 TCGA
XhoI 691 CTCGAG C^TCGAG
809 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
PstI PvuII Sau96I ScrFI SfaNI
SfcI SmlI StyI TseI TspRI
XcmI