Restriction map for
pFN21AE9694
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FokI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 54 GTCTAC GT^[AC][GT]AC
AfaI 41 GTAC GT^AC
AflII 919 CTTAAG C^TTAAG
AlwI 679 GATCC GGATC(4/5)
AlwNI 143 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 520 GAGCTC G[AT]GC[AT]^C
AvaI 314 CTCGGG C^[CT]CG[GA]G
BclI 729 TGATCA T^GATCA
BfrI 919 CTTAAG C^TTAAG
BlpI 282 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 282 GCTGAGC GC^T[ACGT]AGC
BsaI 697 GAGACC GGTCTC(1/5)
BsaWI 92 ACCGGA [AT]^CCGG[AT]
BsiHKAI 520 GAGCTC G[AT]GC[AT]^C
BsmBI 423 GAGACG CGTCTC(1/5)
BsmFI 641 GGGAC GGGAC(10/14)
BsoBI 314 CTCGGG C^[CT]CG[GA]G
BsrBI 285 GAGCGG CCGCTC(-3/-3)
BsrI 239 CCAGT ACTGG(1/-1)
CelII 282 GCTGAGC GC^T[ACGT]AGC
CfoI 280 GCGC GCG^C
DraI 162 TTTAAA TTT^AAA
EaeI 291 CGGCCA [CT]^GGCC[GA]
EarI 517 GAAGAG CTCTTC(1/4)
FbaI 729 TGATCA T^GATCA
HhaI 280 GCGC GCG^C
HinP1I 280 GCGC G^CGC
HindIII 778 AAGCTT A^AGCTT
Ksp632I 517 GAAGAG CTCTTC(1/4)
MboII 517 GAAGA GAAGA(8/7)
MspA1I 538 CAGCTG C[AC]G^C[GT]G
NaeI 297 GCCGGC GCC^GGC
NgoMIV 297 GCCGGC G^CCGGC
PpuMI 602 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 538 CAGCTG CAG^CTG
RsaI 41 GTAC GT^AC
SacI 520 GAGCTC GAGCT^C
SapI 517 GAAGAGC GCTCTTC(1/4)
SfaNI 706 GCATC GCATC(5/9)
StuI 821 AGGCCT AGG^CCT
TaqI 359 TCGA T^CGA
Tsp509I 657 AATT ^AATT
TthHB8I 359 TCGA T^CGA
XcmI 797 CCAAAAAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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BanII 200 GGGCTC G[GA]GC[CT]^C
520 GAGCTC
BfaI 86 CTAG C^TAG
868 CTAG
BmyI 200 GGGCTC G[AGT]GC[ACT]^C
520 GAGCTC
BpmI 628 CTGGAG CTGGAG(16/14)
838 CTGGAG
Bsp1286I 200 GGGCTC G[AGT]GC[ACT]^C
520 GAGCTC
DpnII 679 GATC ^GATC
730 GATC
DraII 602 AGGACCT [GA]G^G[ACGT]CC[CT]
671 AGGGCCT
EcoO109I 602 AGGACCT [GA]G^G[ACGT]CC[CT]
671 AGGGCCT
HincII 553 GTCAAC GT[CT]^[GA]AC
576 GTTGAC
HindII 553 GTCAAC GT[CT]^[GA]AC
576 GTTGAC
HphI 694 GGTGA GGTGA(8/7)
413 TCACC
MaeI 86 CTAG C^TAG
868 CTAG
MboI 679 GATC ^GATC
730 GATC
NdeII 679 GATC ^GATC
730 GATC
NlaIII 47 CATG CATG^
735 CATG
NlaIV 6 GGCTCC GG[ACGT]^[ACGT]CC
201 GGCTCC
PstI 149 CTGCAG CTGCA^G
265 CTGCAG
Sau3AI 679 GATC ^GATC
730 GATC
SmlI 523 CTCAAG C^T[CT][GA]AG
919 CTTAAG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BseRI BsiYI BslI BsmAI BsrFI
BssKI BstNI Cac8I Cfr10I Cfr13I
DdeI EcoRII FauI Fnu4HI HaeIII
HapII HinfI HpaII ItaI MaeIII
MnlI MseI MspI MvaI MwoI
PleI Sau96I ScrFI SfcI TfiI
Tru9I TseI TspRI