Restriction map for
pFN21AE1783
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaII AvrII BalI BamHI
BbeI BbvCI BcgI BciVI BfaI
BfrI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 709 GTCTAC GT^[AC][GT]AC
AflIII 488 ACGTGT A^C[GA][CT]GT
AlwI 255 GATCC GGATC(4/5)
AlwNI 652 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 526 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 331 GGCACC G^G[CT][GA]CC
BanII 220 GAGCTC G[GA]GC[CT]^C
BbrPI 517 CACGTG CAC^GTG
BbsI 532 GTCTTC GAAGAC(2/6)
BclI 26 TGATCA T^GATCA
BlpI 681 GCTGAGC GC^T[ACGT]AGC
BpmI 691 CTGGAG CTGGAG(16/14)
Bpu1102I 681 GCTGAGC GC^T[ACGT]AGC
BpuAI 532 GTCTTC GAAGAC(2/6)
BsaAI 517 CACGTG [CT]AC^GT[GA]
BsrI 57 ACTGG ACTGG(1/-1)
BssHII 664 GCGCGC G^CGCGC
BstEII 385 GGTCACC G^GT[ACGT]ACC
BstPI 385 GGTCACC G^GT[ACGT]ACC
BstYI 254 AGATCC [GA]^GATC[CT]
CelII 681 GCTGAGC GC^T[ACGT]AGC
EcoO65I 385 GGTCACC G^GT[ACGT]ACC
FbaI 26 TGATCA T^GATCA
HincII 139 GTTGAC GT[CT]^[GA]AC
HindII 139 GTTGAC GT[CT]^[GA]AC
HinfI 325 GAGTC G^A[ACGT]TC
MboII 533 TCTTC GAAGA(8/7)
MflI 254 AGATCC [GA]^GATC[CT]
NaeI 772 GCCGGC GCC^GGC
NcoI 63 CCATGG C^CATGG
NgoMIV 772 GCCGGC G^CCGGC
NspI 646 ACATGC [GA]CATG^[CT]
PflFI 526 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 651 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 325 GAGTC GAGTC(4/5)
PmaCI 517 CACGTG CAC^GTG
PmlI 517 CACGTG CAC^GTG
SacI 220 GAGCTC GAGCT^C
SfaNI 626 GCATC GCATC(5/9)
SmaI 431 CCCGGG CCC^GGG
SmlI 586 CTCAAG C^T[CT][GA]AG
Tth111I 526 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 651 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 254 AGATCC [GA]^GATC[CT]
XmaCI 431 CCCGGG C^CCGGG
XmaI 431 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 665 CGCG CG^CG
667 CGCG
AcyI 526 GACGCC G[GA]^CG[CT]C
580 GACGCC
AvaI 217 CCCGAG C^[CT]CG[GA]G
431 CCCGGG
AviII 476 TGCGCA TGC^GCA
719 TGCGCA
BsaHI 526 GACGCC G[GA]^CG[CT]C
580 GACGCC
BseRI 19 GAGGAG GAGGAG(10/8)
436 GAGGAG
BsiEI 150 CGGCCG CG[GA][CT]^CG
180 CGGCCG
BsmFI 578 GGGAC GGGAC(10/14)
351 GTCCC
BsoBI 217 CCCGAG C^[CT]CG[GA]G
431 CCCGGG
BsrDI 372 CATTGC GCAATG(2/0)
70 GCAATG
BstUI 665 CGCG CG^CG
667 CGCG
BstXI 287 CCATGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
547 CCATCACCCTGG
DpnII 27 GATC ^GATC
255 GATC
DraIII 394 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
451 CACCGTGTG
EagI 150 CGGCCG C^GGCCG
180 CGGCCG
EclXI 150 CGGCCG C^GGCCG
180 CGGCCG
Eco52I 150 CGGCCG C^GGCCG
180 CGGCCG
EcoT14I 63 CCATGG C^C[AT][AT]GG
167 CCAAGG
FauI 417 GCGGG CCCGC(4/6)
599 GCGGG
FspI 476 TGCGCA TGC^GCA
719 TGCGCA
HaeII 602 GGCGCT [GA]GCGC^[CT]
654 GGCGCT
Hin1I 526 GACGCC G[GA]^CG[CT]C
580 GACGCC
MboI 27 GATC ^GATC
255 GATC
MvnI 665 CGCG CG^CG
667 CGCG
NdeII 27 GATC ^GATC
255 GATC
Sau3AI 27 GATC ^GATC
255 GATC
StyI 63 CCATGG C^C[AT][AT]GG
167 CCAAGG
TaqI 593 TCGA T^CGA
629 TCGA
TthHB8I 593 TCGA T^CGA
629 TCGA
XcmI 47 CCATCCTGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
108 CCAGACCCGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AspHI BbvI BcnI
BglI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DsaI EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TseI
Tsp45I TspRI