Restriction map for
pFN21AE5195
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I CelII CfoI ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MboII MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfaNI SfiI
SfoI SfuI SgrAI SmaI SpeI
Sse8387I SspI StuI SwaI TaqI
Tsp45I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AciI 239 CCGC CCGC(-3/-1)
AcsI 119 AAATTC [GA]^AATT[CT]
AluI 329 AGCT AG^CT
AlwI 69 GATCC GGATC(4/5)
AocI 335 CCTCAGG CC^T[ACGT]AGG
ApaI 304 GGGCCC GGGCC^C
ApoI 119 AAATTC [GA]^AATT[CT]
AspI 457 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 3 GGCACC G^G[CT][GA]CC
BanII 304 GGGCCC G[GA]GC[CT]^C
BciVI 345 GTATCC GTATCC(6/5)
BmyI 304 GGGCCC G[AGT]GC[ACT]^C
BpmI 228 CTCCAG CTGGAG(16/14)
BsaAI 388 TACGTA [CT]AC^GT[GA]
BsgI 214 CTGCAC GTGCAG(16/14)
BsiYI 411 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 411 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 312 GTCCC GGGAC(10/14)
Bsp1286I 304 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 447 TGTACA T^GTACA
BsrFI 6 ACCGGC [GA]^CCGG[CT]
BsrGI 447 TGTACA T^GTACA
BssKI 438 CCTGG ^CC[ACGT]GG
BssSI 217 CACGAG CACGAG(-5/-1)
BstNI 438 CCTGG CC^[AT]GG
BstYI 68 AGATCC [GA]^GATC[CT]
Bsu36I 335 CCTCAGG CC^T[ACGT]AGG
Cfr10I 6 ACCGGC [GA]^CCGG[CT]
DpnII 69 GATC ^GATC
DraI 115 TTTAAA TTT^AAA
DsaI 300 CCACGG C^C[GA][CT]GG
Eco81I 335 CCTCAGG CC^T[ACGT]AGG
EcoRII 438 CCTGG ^CC[AT]GG
EcoT14I 89 CCAAGG C^C[AT][AT]GG
HapII 7 CCGG C^CGG
HpaII 7 CCGG C^CGG
HphI 332 TCACC GGTGA(8/7)
MaeII 389 ACGT A^CGT
MboI 69 GATC ^GATC
MflI 68 AGATCC [GA]^GATC[CT]
MslI 104 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 7 CCGG C^CGG
MvaI 438 CCTGG CC^[AT]GG
NdeII 69 GATC ^GATC
PflFI 457 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 354 CTGCAG CTGCA^G
Sau3AI 69 GATC ^GATC
ScrFI 438 CCTGG CC^[ACGT]GG
SexAI 437 ACCTGGT A^CC[AT]GGT
SfcI 354 CTGCAG C^T[GA][CT]AG
SmlI 143 CTCAAG C^T[CT][GA]AG
SnaBI 388 TACGTA TAC^GTA
SphI 148 GCATGC GCATG^C
SspBI 447 TGTACA T^GTACA
StyI 89 CCAAGG C^C[AT][AT]GG
TfiI 20 GATTC G^A[AT]TC
Tth111I 457 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 68 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 360 GTAC GT^AC
448 GTAC
BsaJI 89 CCAAGG C^C[ACGT][ACGT]GG
300 CCACGG
BspMI 244 ACCTGC ACCTGC(4/8)
356 GCAGGT
BsrI 73 CCAGT ACTGG(1/-1)
230 CCAGT
Cac8I 148 GCATGC GC[ACGT]^[ACGT]GC
265 GCCTGC
Cfr13I 304 GGGCC G^G[ACGT]CC
305 GGCCC
EaeI 8 CGGCCA [CT]^GGCC[GA]
236 TGGCCG
MseI 116 TTAA T^TAA
173 TTAA
NspI 128 ACATGC [GA]CATG^[CT]
148 GCATGC
PleI 421 GACTC GAGTC(4/5)
461 GAGTC
RsaI 360 GTAC GT^AC
448 GTAC
Sau96I 304 GGGCC G^G[ACGT]CC
305 GGCCC
Tru9I 116 TTAA T^TAA
173 TTAA
Tsp509I 120 AATT ^AATT
431 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
BbvI DdeI Fnu4HI HaeIII HinfI
ItaI MnlI MwoI NlaIII NlaIV
TseI TspRI