Restriction map for
pFN21AE1624
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SalI SbfI ScaI SexAI SfaNI
SfcI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 285 GAATTC [GA]^AATT[CT]
AhdI 121 GACTTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 285 GAATTC [GA]^AATT[CT]
Asp700I 255 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 121 GACTTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 163 GAGCAC G[AT]GC[AT]^C
AvaII 78 GGACC G^G[AT]CC
BcnI 51 CCCGG CC^[GC]GG
BfaI 367 CTAG C^TAG
BglI 34 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 163 GAGCAC G[AGT]GC[ACT]^C
BpmI 169 CTGGAG CTGGAG(16/14)
BsgI 236 GTGCAG GTGCAG(16/14)
BsiHKAI 163 GAGCAC G[AT]GC[AT]^C
BsmAI 353 GAGAC GTCTC(1/5)
Bsp1286I 163 GAGCAC G[AGT]GC[ACT]^C
BsrDI 378 CATTGC GCAATG(2/0)
BssSI 139 CACGAG CACGAG(-5/-1)
DdeI 335 CTCAG C^T[ACGT]AG
DpnII 385 GATC ^GATC
DraII 77 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 121 GACTTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 311 CTCTTC CTCTTC(1/4)
EcoO109I 77 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 285 GAATTC G^AATTC
HapII 52 CCGG C^CGG
HpaII 52 CCGG C^CGG
Ksp632I 311 CTCTTC CTCTTC(1/4)
MaeI 367 CTAG C^TAG
MaeIII 328 GTCAC ^GT[ACGT]AC
MboI 385 GATC ^GATC
MboII 312 TCTTC GAAGA(8/7)
MspI 52 CCGG C^CGG
NciI 51 CCCGG CC^[GC]GG
NdeII 385 GATC ^GATC
NlaIII 177 CATG CATG^
PpuMI 77 AGGACCT [GA]G^G[AT]CC[CT]
SapI 310 GCTCTTC GCTCTTC(1/4)
Sau3AI 385 GATC ^GATC
SfiI 33 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 241 GATTC G^A[AT]TC
Tsp45I 328 GTCAC ^GT[GC]AC
XmnI 255 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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BseRI 332 CTCCTC GAGGAG(10/8)
160 GAGGAG
BstNI 57 CCTGG CC^[AT]GG
168 CCTGG
Cfr13I 54 GGCCC G^G[ACGT]CC
78 GGACC
EcoRII 57 CCTGG ^CC[AT]GG
168 CCTGG
FauI 10 CCCGC CCCGC(4/6)
28 CCCGC
HaeII 143 AGCGCC [GA]GCGC^[CT]
193 AGCGCC
MvaI 57 CCTGG CC^[AT]GG
168 CCTGG
Sau96I 54 GGCCC G^G[ACGT]CC
78 GGACC
Tsp509I 286 AATT ^AATT
347 AATT
TspRI 270 CAGTG CA[GC]TG[ACGT][ACGT]^
324 CACTG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiEI BsiYI BslI BssKI BstUI
Cac8I CfoI DsaI EaeI EagI
EclXI Eco52I Fnu4HI HaeIII HgaI
HhaI HinP1I HinfI ItaI KspI
MnlI MspA1I MvnI MwoI NotI
PleI SacII ScrFI TseI