Restriction map for
pFN21AE5393
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BcgI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspEI BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MamI MluI
MroI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcsI 249 GAATTT [GA]^AATT[CT]
ApoI 249 GAATTT [GA]^AATT[CT]
Asp700I 705 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 758 GGACC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BbvCI 691 GCTGAGG CCTCAGC(-5/-2)
BbvI 305 GCTGC GCAGC(8/12)
BciVI 153 GGATAC GTATCC(6/5)
BclI 839 TGATCA T^GATCA
BseRI 550 CTCCTC GAGGAG(10/8)
BsmFI 757 GGGAC GGGAC(10/14)
Bsp1407I 257 TGTACA T^GTACA
BspHI 320 TCATGA T^CATGA
BspLU11I 653 ACATGT A^CATGT
BsrBI 149 GAGCGG CCGCTC(-3/-3)
BsrGI 257 TGTACA T^GTACA
BstYI 593 GGATCT [GA]^GATC[CT]
Cac8I 735 GCTGGC GC[ACGT]^[ACGT]GC
DraII 757 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoO109I 757 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 324 GATATC GAT^ATC
EcoT14I 350 CCATGG C^C[AT][AT]GG
FbaI 839 TGATCA T^GATCA
Fnu4HI 305 GCTGC GC^[ACGT]GC
HincII 520 GTCAAC GT[CT]^[GA]AC
HindII 520 GTCAAC GT[CT]^[GA]AC
ItaI 305 GCTGC GC^[ACGT]GC
MaeII 718 ACGT A^CGT
MfeI 12 CAATTG C^AATTG
MflI 593 GGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 862 TTAA T^TAA
MspA1I 689 CAGCTG C[AC]G^C[GT]G
MunI 12 CAATTG C^AATTG
MwoI 346 GCTTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 350 CCATGG C^CATGG
PflMI 795 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 408 GACTC GAGTC(4/5)
PpuMI 757 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 689 CAGCTG CAG^CTG
RcaI 320 TCATGA T^CATGA
SspBI 257 TGTACA T^GTACA
StyI 350 CCATGG C^C[AT][AT]GG
TaqI 715 TCGA T^CGA
Tru9I 862 TTAA T^TAA
TseI 305 GCTGC G^C[AT]GC
Tsp45I 742 GTCAC ^GT[GC]AC
TthHB8I 715 TCGA T^CGA
Van91I 795 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 593 GGATCT [GA]^GATC[CT]
XmnI 705 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 492 CCGC CCGC(-3/-1)
151 GCGG
AflIII 653 ACATGT A^C[GA][CT]GT
718 ACGTGT
AlwI 135 GATCC GGATC(4/5)
593 GGATC
AocI 552 CCTCAGG CC^T[ACGT]AGG
675 CCTGAGG
BbsI 698 GAAGAC GAAGAC(2/6)
826 GTCTTC
BpmI 410 CTCCAG CTGGAG(16/14)
874 CTCCAG
BpuAI 698 GAAGAC GAAGAC(2/6)
826 GTCTTC
BsmAI 161 GTCTC GTCTC(1/5)
646 GTCTC
BsmI 422 GCATTC GAATGC(1/-1)
684 GCATTC
BsrI 300 ACTGG ACTGG(1/-1)
342 ACTGG
Bsu36I 552 CCTCAGG CC^T[ACGT]AGG
675 CCTGAGG
Cfr13I 45 GGGCC G^G[ACGT]CC
758 GGACC
EarI 106 CTCTTC CTCTTC(1/4)
219 CTCTTC
Eco81I 552 CCTCAGG CC^T[ACGT]AGG
675 CCTGAGG
EcoT22I 284 ATGCAT ATGCA^T
682 ATGCAT
Ksp632I 106 CTCTTC CTCTTC(1/4)
219 CTCTTC
NlaIV 757 GGGACC GG[ACGT]^[ACGT]CC
758 GGACCC
NsiI 284 ATGCAT ATGCA^T
682 ATGCAT
Sau96I 45 GGGCC G^G[ACGT]CC
758 GGACC
SfcI 450 CTATAG C^T[GA][CT]AG
867 CTATAG
Tsp509I 13 AATT ^AATT
250 AATT
TspRI 113 CAGTG CA[GC]TG[ACGT][ACGT]^
836 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BfaI BsaJI BsiYI
BslI BssKI BstF5I BstNI DdeI
DpnII EcoRII FokI HaeIII HinfI
HphI MaeI MaeIII MboI MboII
MnlI MslI MvaI NdeII NlaIII
NspI RsaI Sau3AI ScrFI SfaNI
TfiI