Restriction map for
pFN21AE3956
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI FseI FspI
HpaI KasI KpnI KspI MamI
MluI MroI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 57 CGCG CG^CG
AciI 56 CCGC CCGC(-3/-1)
AcyI 626 GGCGTC G[GA]^CG[CT]C
AlwNI 206 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 300 AGCGCT AGC^GCT
AseI 595 ATTAAT AT^TAAT
AsnI 595 ATTAAT AT^TAAT
AvaI 272 CTCGAG C^[CT]CG[GA]G
AvaII 516 GGACC G^G[AT]CC
BanI 167 GGCACC G^G[CT][GA]CC
BclI 1365 TGATCA T^GATCA
BcnI 70 CCGGG CC^[GC]GG
BfaI 1480 CTAG C^TAG
BglII 1215 AGATCT A^GATCT
BsaAI 105 CACGTA [CT]AC^GT[GA]
BsaHI 626 GGCGTC G[GA]^CG[CT]C
BsaJI 722 CCATGG C^C[ACGT][ACGT]GG
BsgI 10 GTGCAG GTGCAG(16/14)
BsoBI 272 CTCGAG C^[CT]CG[GA]G
BspMI 456 ACCTGC ACCTGC(4/8)
BsrI 506 CCAGT ACTGG(1/-1)
BstUI 57 CGCG CG^CG
Cac8I 543 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 516 GGACC G^G[ACGT]CC
DraII 515 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 722 CCATGG C^C[GA][CT]GG
Eco47III 300 AGCGCT AGC^GCT
EcoO109I 515 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 493 GATATC GAT^ATC
EcoT14I 722 CCATGG C^C[AT][AT]GG
EcoT22I 473 ATGCAT ATGCA^T
FauI 55 CCCGC CCCGC(4/6)
FbaI 1365 TGATCA T^GATCA
HaeII 300 AGCGCT [GA]GCGC^[CT]
HapII 70 CCGG C^CGG
HgaI 627 GCGTC GACGC(5/10)
Hin1I 626 GGCGTC G[GA]^CG[CT]C
HpaII 70 CCGG C^CGG
MaeI 1480 CTAG C^TAG
MspI 70 CCGG C^CGG
MvnI 57 CGCG CG^CG
NciI 70 CCGGG CC^[GC]GG
NcoI 722 CCATGG C^CATGG
NsiI 473 ATGCAT ATGCA^T
PaeR7I 272 CTCGAG C^TCGAG
PflMI 834 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 515 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 595 ATTAAT AT^TAAT
Sau96I 516 GGACC G^G[ACGT]CC
StuI 64 AGGCCT AGG^CCT
StyI 722 CCATGG C^C[AT][AT]GG
TspRI 78 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 834 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 272 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 629 GTCTAC GT^[AC][GT]AC
643 GTAGAC
AfaI 688 GTAC GT^AC
1225 GTAC
Asp700I 325 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
820 GAATACCTTC
BalI 2 TGGCCA TGG^CCA
1304 TGGCCA
BsiYI 56 CCGCGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCAAATGGTGG
BslI 56 CCGCGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCAAATGGTGG
BsmAI 1529 GAGAC GTCTC(1/5)
582 GTCTC
BstF5I 488 CATCC GGATG(2/0)
216 GGATG
BstNI 461 CCAGG CC^[AT]GG
1267 CCTGG
BstYI 1215 AGATCT [GA]^GATC[CT]
1323 AGATCC
EaeI 2 TGGCCA [CT]^GGCC[GA]
1304 TGGCCA
EarI 134 CTCTTC CTCTTC(1/4)
1319 GAAGAG
EcoRII 461 CCAGG ^CC[AT]GG
1267 CCTGG
FokI 488 CATCC GGATG(9/13)
216 GGATG
HincII 727 GTCAAC GT[CT]^[GA]AC
1183 GTTGAC
HindII 727 GTCAAC GT[CT]^[GA]AC
1183 GTTGAC
Ksp632I 134 CTCTTC CTCTTC(1/4)
1319 GAAGAG
MfeI 620 CAATTG C^AATTG
806 CAATTG
MflI 1215 AGATCT [GA]^GATC[CT]
1323 AGATCC
MluNI 2 TGGCCA TGG^CCA
1304 TGGCCA
MscI 2 TGGCCA TGG^CCA
1304 TGGCCA
MunI 620 CAATTG C^AATTG
806 CAATTG
MvaI 461 CCAGG CC^[AT]GG
1267 CCTGG
PleI 661 GACTC GAGTC(4/5)
775 GACTC
RsaI 688 GTAC GT^AC
1225 GTAC
SmlI 272 CTCGAG C^T[CT][GA]AG
760 CTCAAG
TaqI 273 TCGA T^CGA
750 TCGA
TthHB8I 273 TCGA T^CGA
750 TCGA
XhoII 1215 AGATCT [GA]^GATC[CT]
1323 AGATCC
XmnI 325 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
820 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbsI
BbvI BpuAI BssKI CfoI DdeI
DpnII DraI Fnu4HI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I