Restriction map for
pFN21AE4181
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
FbaI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 202 CGCG CG^CG
AccIII 1355 TCCGGA T^CCGGA
AflIII 528 ACGTGT A^C[GA][CT]GT
AspI 1222 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 644 TGCGCA TGC^GCA
BbsI 838 GAAGAC GAAGAC(2/6)
BcgI 1610 GCAAGATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 462 AGATCT A^GATCT
BpuAI 838 GAAGAC GAAGAC(2/6)
BsaWI 1355 TCCGGA [AT]^CCGG[AT]
BseAI 1355 TCCGGA T^CCGGA
BsmI 1743 GAATGC GAATGC(1/-1)
BspEI 1355 TCCGGA T^CCGGA
BspHI 1208 TCATGA T^CATGA
BsrBI 85 GAGCGG CCGCTC(-3/-3)
BsrFI 290 ACCGGC [GA]^CCGG[CT]
BstEII 1557 GGTGACC G^GT[ACGT]ACC
BstPI 1557 GGTGACC G^GT[ACGT]ACC
BstUI 202 CGCG CG^CG
CfoI 645 GCGC GCG^C
Cfr10I 290 ACCGGC [GA]^CCGG[CT]
DraI 865 TTTAAA TTT^AAA
DraIII 1022 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 438 CCATGG C^C[GA][CT]GG
EaeI 137 CGGCCA [CT]^GGCC[GA]
EcoO65I 1557 GGTGACC G^GT[ACGT]ACC
EcoRV 748 GATATC GAT^ATC
EcoT14I 438 CCATGG C^C[AT][AT]GG
EcoT22I 1538 ATGCAT ATGCA^T
FspI 644 TGCGCA TGC^GCA
HhaI 645 GCGC GCG^C
HinP1I 645 GCGC G^CGC
MaeII 528 ACGT A^CGT
MroI 1355 TCCGGA T^CCGGA
MvnI 202 CGCG CG^CG
NcoI 438 CCATGG C^CATGG
NsiI 1538 ATGCAT ATGCA^T
PflFI 1222 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 624 GACTC GAGTC(4/5)
PpuMI 1621 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 260 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1208 TCATGA T^CATGA
ScaI 629 AGTACT AGT^ACT
SmlI 1780 CTCAAG C^T[CT][GA]AG
SphI 1421 GCATGC GCATG^C
SspI 1095 AATATT AAT^ATT
StyI 438 CCATGG C^C[AT][AT]GG
TaqI 152 TCGA T^CGA
Tth111I 1222 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 152 TCGA T^CGA
XbaI 1833 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 862 GAATTT [GA]^AATT[CT]
1786 AAATTT
AfaI 212 GTAC GT^AC
630 GTAC
AocI 351 CCTGAGG CC^T[ACGT]AGG
472 CCTCAGG
ApaI 77 GGGCCC GGGCC^C
845 GGGCCC
ApoI 862 GAATTT [GA]^AATT[CT]
1786 AAATTT
AvaII 1344 GGTCC G^G[AT]CC
1622 GGTCC
BfaI 1478 CTAG C^TAG
1834 CTAG
BsaI 562 GAGACC GGTCTC(1/5)
608 GAGACC
BstAPI 118 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1021 GCACTGAGTGC
Bsu36I 351 CCTGAGG CC^T[ACGT]AGG
472 CCTCAGG
Eco81I 351 CCTGAGG CC^T[ACGT]AGG
472 CCTCAGG
MaeI 1478 CTAG C^TAG
1834 CTAG
MslI 439 CATGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1541 CATTTTGGTG
NspI 47 ACATGC [GA]CATG^[CT]
1421 GCATGC
PstI 116 CTGCAG CTGCA^G
1390 CTGCAG
RsaI 212 GTAC GT^AC
630 GTAC
SmaI 74 CCCGGG CCC^GGG
80 CCCGGG
StuI 549 AGGCCT AGG^CCT
1386 AGGCCT
TfiI 1331 GAATC G^A[AT]TC
1858 GAATC
XcmI 1534 CCAGATGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1562 CCATGACAACTGTGG
XmaCI 74 CCCGGG C^CCGGG
80 CCCGGG
XmaI 74 CCCGGG C^CCGGG
80 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII