Restriction map for
pFN21AE6257
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BciVI BfaI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MunI
MwoI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflII 280 CTTAAG C^TTAAG
AgeI 182 ACCGGT A^CCGGT
AlwNI 656 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 443 GGATCC G^GATCC
BanII 673 GGGCTC G[GA]GC[CT]^C
BbsI 391 GTCTTC GAAGAC(2/6)
BcgI 26 GCACTTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 65 TGATCA T^GATCA
BfrI 280 CTTAAG C^TTAAG
BmyI 673 GGGCTC G[AGT]GC[ACT]^C
BpuAI 391 GTCTTC GAAGAC(2/6)
BsaWI 182 ACCGGT [AT]^CCGG[AT]
BseRI 549 GAGGAG GAGGAG(10/8)
BsgI 24 CTGCAC GTGCAG(16/14)
BsiEI 180 CGACCG CG[GA][CT]^CG
BsmBI 552 GAGACG CGTCTC(1/5)
Bsp1286I 673 GGGCTC G[AGT]GC[ACT]^C
BsrFI 182 ACCGGT [GA]^CCGG[CT]
BstYI 443 GGATCC [GA]^GATC[CT]
Cfr10I 182 ACCGGT [GA]^CCGG[CT]
DdeI 310 CTGAG C^T[ACGT]AG
DrdI 177 GACCGACCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 84 CCCGC CCCGC(4/6)
FbaI 65 TGATCA T^GATCA
MflI 443 GGATCC [GA]^GATC[CT]
MseI 281 TTAA T^TAA
MspA1I 692 CAGCTG C[AC]G^C[GT]G
PinAI 182 ACCGGT A^CCGGT
PvuII 692 CAGCTG CAG^CTG
SfcI 244 CTACAG C^T[GA][CT]AG
SmlI 280 CTTAAG C^T[CT][GA]AG
SspI 406 AATATT AAT^ATT
Tru9I 281 TTAA T^TAA
XcmI 338 CCATCCGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 443 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 204 GTAC GT^AC
558 GTAC
AluI 275 AGCT AG^CT
693 AGCT
AlwI 444 GATCC GGATC(4/5)
443 GGATC
BcnI 45 CCCGG CC^[GC]GG
194 CCCGG
BpmI 199 CTCCAG CTGGAG(16/14)
695 CTGGAG
BsaI 129 GGTCTC GGTCTC(1/5)
364 GGTCTC
BsrI 560 ACTGG ACTGG(1/-1)
201 CCAGT
BstF5I 99 CATCC GGATG(2/0)
339 CATCC
Cac8I 634 GCCAGC GC[ACGT]^[ACGT]GC
638 GCCTGC
CfoI 55 GCGC GCG^C
720 GCGC
DraII 80 GGGACCC [GA]G^G[ACGT]CC[CT]
700 GGGTCCC
DsaI 447 CCATGG C^C[GA][CT]GG
668 CCATGG
EcoNI 240 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
542 CCTGTGAGAGG
EcoO109I 80 GGGACCC [GA]G^G[ACGT]CC[CT]
700 GGGTCCC
EcoT14I 447 CCATGG C^C[AT][AT]GG
668 CCATGG
FokI 99 CATCC GGATG(9/13)
339 CATCC
HhaI 55 GCGC GCG^C
720 GCGC
HinP1I 55 GCGC G^CGC
720 GCGC
HinfI 454 GACTC G^A[ACGT]TC
585 GAGTC
MboII 323 GAAGA GAAGA(8/7)
392 TCTTC
NciI 45 CCCGG CC^[GC]GG
194 CCCGG
NcoI 447 CCATGG C^CATGG
668 CCATGG
PleI 454 GACTC GAGTC(4/5)
585 GAGTC
PpuMI 80 GGGACCC [GA]G^G[AT]CC[CT]
700 GGGTCCC
RsaI 204 GTAC GT^AC
558 GTAC
StyI 447 CCATGG C^C[AT][AT]GG
668 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AvaII BbvI BsaJI
BsiYI BslI BsmAI BsmFI BssKI
BstNI BstUI Cfr13I DpnII EcoRII
Fnu4HI HaeIII HapII HpaII HphI
ItaI MaeIII MboI MnlI MspI
MvaI MvnI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI