Restriction map for
pFN21AE9422
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AlwNI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BlnI BpmI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI FbaI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 259 GTAC GT^AC
AhdI 72 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1915 GTGCAC G^TGCAC
ApaLI 1915 GTGCAC G^TGCAC
AseI 376 ATTAAT AT^TAAT
AsnI 376 ATTAAT AT^TAAT
AspEI 72 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1882 GGCACC G^G[CT][GA]CC
BbsI 1152 GTCTTC GAAGAC(2/6)
BglII 456 AGATCT A^GATCT
BlpI 1689 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1689 GCTTAGC GC^T[ACGT]AGC
BpuAI 1152 GTCTTC GAAGAC(2/6)
BsaI 1186 GAGACC GGTCTC(1/5)
BsmI 333 GAATGC GAATGC(1/-1)
BsrBI 13 CCGCTC CCGCTC(-3/-3)
CelII 1689 GCTTAGC GC^T[ACGT]AGC
DsaI 1625 CCGTGG C^C[GA][CT]GG
Eam1105I 72 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 705 GATATC GAT^ATC
EcoT14I 545 CCAAGG C^C[AT][AT]GG
EcoT22I 1332 ATGCAT ATGCA^T
FauI 12 CCCGC CCCGC(4/6)
HapII 1481 CCGG C^CGG
HpaII 1481 CCGG C^CGG
MspI 1481 CCGG C^CGG
NsiI 1332 ATGCAT ATGCA^T
NspI 1732 GCATGC [GA]CATG^[CT]
PshBI 376 ATTAAT AT^TAAT
PstI 1799 CTGCAG CTGCA^G
PvuII 1146 CAGCTG CAG^CTG
RsaI 259 GTAC GT^AC
SacI 1020 GAGCTC GAGCT^C
SfaNI 1921 GCATC GCATC(5/9)
SphI 1732 GCATGC GCATG^C
StyI 545 CCAAGG C^C[AT][AT]GG
TaqI 1077 TCGA T^CGA
TthHB8I 1077 TCGA T^CGA
XbaI 2002 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 13 CCGC CCGC(-3/-1)
1203 CCGC
AspHI 1020 GAGCTC G[AT]GC[AT]^C
1915 GTGCAC
BanII 1020 GAGCTC G[GA]GC[CT]^C
1856 GAGCCC
BciVI 612 GGATAC GTATCC(6/5)
1654 GGATAC
BseRI 979 GAGGAG GAGGAG(10/8)
1853 GAGGAG
BsiHKAI 1020 GAGCTC G[AT]GC[AT]^C
1915 GTGCAC
BsiYI 1249 CCTTCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1620 CCCAGCCGTGG
BslI 1249 CCTTCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1620 CCCAGCCGTGG
BsmFI 629 GGGAC GGGAC(10/14)
2044 GTCCC
MslI 1517 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1551 CATTGTTGTG
MspA1I 1146 CAGCTG C[AC]G^C[GT]G
1203 CCGCTG
SapI 1461 GCTCTTC GCTCTTC(1/4)
1864 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
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AcsI AluI AlwI ApoI AvaII
BbvI BfaI BmyI BsaJI BsmAI
Bsp1286I BsrDI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfcI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII