Restriction map for
pFN21AE5976
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HindIII HpaII HphI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 771 GTATAC GT^[AC][GT]AC
AocI 686 CCTCAGG CC^T[ACGT]AGG
AspHI 97 GTGCTC G[AT]GC[AT]^C
BalI 188 TGGCCA TGG^CCA
BanII 408 GAGCCC G[GA]GC[CT]^C
BbsI 655 GTCTTC GAAGAC(2/6)
BpuAI 655 GTCTTC GAAGAC(2/6)
BsiHKAI 97 GTGCTC G[AT]GC[AT]^C
BsmAI 194 GTCTC GTCTC(1/5)
BsmFI 45 GGGAC GGGAC(10/14)
BssKI 443 CCTGG ^CC[ACGT]GG
Bst1107I 771 GTATAC GTA^TAC
BstNI 443 CCTGG CC^[AT]GG
BstXI 312 CCACGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 771 GTATAC GTA^TAC
Bsu36I 686 CCTCAGG CC^T[ACGT]AGG
CfoI 264 GCGC GCG^C
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 16 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 350 CCATGG C^C[GA][CT]GG
Eco81I 686 CCTCAGG CC^T[ACGT]AGG
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 443 CCTGG ^CC[AT]GG
HhaI 264 GCGC GCG^C
HinP1I 264 GCGC G^CGC
HincII 601 GTTAAC GT[CT]^[GA]AC
HindII 601 GTTAAC GT[CT]^[GA]AC
HpaI 601 GTTAAC GTT^AAC
MluNI 188 TGGCCA TGG^CCA
MscI 188 TGGCCA TGG^CCA
MvaI 443 CCTGG CC^[AT]GG
NcoI 350 CCATGG C^CATGG
PleI 47 GACTC GAGTC(4/5)
ScrFI 443 CCTGG CC^[ACGT]GG
SfcI 576 CTACAG C^T[GA][CT]AG
XcmI 312 CCACGTCATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 66 GATCC GGATC(4/5)
929 GGATC
BmyI 97 GTGCTC G[AGT]GC[ACT]^C
408 GAGCCC
BseRI 73 CTCCTC GAGGAG(10/8)
76 CTCCTC
Bsp1286I 97 GTGCTC G[AGT]GC[ACT]^C
408 GAGCCC
BstYI 65 AGATCC [GA]^GATC[CT]
929 GGATCT
Cac8I 109 GCAAGC GC[ACGT]^[ACGT]GC
217 GCCTGC
DpnII 66 GATC ^GATC
930 GATC
EaeI 188 TGGCCA [CT]^GGCC[GA]
778 CGGCCA
MaeII 314 ACGT A^CGT
653 ACGT
MboI 66 GATC ^GATC
930 GATC
MflI 65 AGATCC [GA]^GATC[CT]
929 GGATCT
MseI 491 TTAA T^TAA
602 TTAA
MslI 345 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
762 CATGTTTGTG
NdeII 66 GATC ^GATC
930 GATC
NlaIV 214 GGAGCC GG[ACGT]^[ACGT]CC
446 GGACCC
Sau3AI 66 GATC ^GATC
930 GATC
SfaNI 823 GATGC GCATC(5/9)
290 GCATC
TfiI 12 GAATC G^A[AT]TC
846 GAATC
Tru9I 491 TTAA T^TAA
602 TTAA
Tsp509I 493 AATT ^AATT
790 AATT
XhoII 65 AGATCC [GA]^GATC[CT]
929 GGATCT
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BsaJI BsrI BstF5I Cfr13I DdeI
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboII MnlI
NlaIII RsaI Sau96I StyI TseI
Tsp45I TspRI