Restriction map for
pFN21AE6563
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I MaeI
MamI MboII MfeI MflI MluI
MroI MseI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 229 CGCG CG^CG
AcyI 194 GGCGCC G[GA]^CG[CT]C
AfaI 8 GTAC GT^AC
ApaI 202 GGGCCC GGGCC^C
AvaI 296 CCCGGG C^[CT]CG[GA]G
BalI 68 TGGCCA TGG^CCA
BbeI 194 GGCGCC GGCGC^C
BbvCI 285 CCTCAGC CCTCAGC(-5/-2)
BclI 125 TGATCA T^GATCA
BglI 352 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 194 GGCGCC G[GA]^CG[CT]C
BsaI 409 GAGACC GGTCTC(1/5)
BsmBI 103 GAGACG CGTCTC(1/5)
BsoBI 296 CCCGGG C^[CT]CG[GA]G
BspHI 330 TCATGA T^CATGA
BsrBI 375 CCGCTC CCGCTC(-3/-3)
BstEII 364 GGTCACC G^GT[ACGT]ACC
BstF5I 416 GGATG GGATG(2/0)
BstPI 364 GGTCACC G^GT[ACGT]ACC
BstUI 229 CGCG CG^CG
DpnII 126 GATC ^GATC
EaeI 68 TGGCCA [CT]^GGCC[GA]
EcoO65I 364 GGTCACC G^GT[ACGT]ACC
FbaI 125 TGATCA T^GATCA
FokI 416 GGATG GGATG(9/13)
Hin1I 194 GGCGCC G[GA]^CG[CT]C
KasI 194 GGCGCC G^GCGCC
KspI 228 CCGCGG CCGC^GG
MaeII 160 ACGT A^CGT
MaeIII 365 GTCAC ^GT[ACGT]AC
MboI 126 GATC ^GATC
MluNI 68 TGGCCA TGG^CCA
MscI 68 TGGCCA TGG^CCA
MvnI 229 CGCG CG^CG
NarI 194 GGCGCC GG^CGCC
NdeII 126 GATC ^GATC
PleI 463 GAGTC GAGTC(4/5)
PvuII 121 CAGCTG CAG^CTG
RcaI 330 TCATGA T^CATGA
RsaI 8 GTAC GT^AC
SacII 228 CCGCGG CCGC^GG
Sau3AI 126 GATC ^GATC
SfaNI 347 GCATC GCATC(5/9)
SfoI 194 GGCGCC GGC^GCC
SmaI 296 CCCGGG CCC^GGG
TfiI 319 GATTC G^A[AT]TC
Tsp45I 365 GTCAC ^GT[GC]AC
XmaCI 296 CCCGGG C^CCGGG
XmaI 296 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanI 194 GGCGCC G^G[CT][GA]CC
212 GGTGCC
BanII 136 GAGCCC G[GA]GC[CT]^C
202 GGGCCC
BsmAI 103 GAGAC GTCTC(1/5)
409 GAGAC
BsmFI 233 GGGAC GGGAC(10/14)
162 GTCCC
BspMI 209 GCAGGT ACCTGC(4/8)
270 GCAGGT
DraII 202 GGGCCCC [GA]G^G[ACGT]CC[CT]
281 AGGGCCT
EcoO109I 202 GGGCCCC [GA]G^G[ACGT]CC[CT]
281 AGGGCCT
EcoT14I 83 CCATGG C^C[AT][AT]GG
198 CCATGG
HaeII 194 GGCGCC [GA]GCGC^[CT]
386 AGCGCC
HinfI 319 GATTC G^A[ACGT]TC
463 GAGTC
MspA1I 121 CAGCTG C[AC]G^C[GT]G
228 CCGCGG
NcoI 83 CCATGG C^CATGG
198 CCATGG
PflMI 44 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
60 CCATCTAATGG
StyI 83 CCATGG C^C[AT][AT]GG
198 CCATGG
Van91I 44 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
60 CCATCTAATGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DsaI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI TseI TspRI