Restriction map for
pFN21AE5334
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Cac8I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 294 CGCG CG^CG
AfaI 139 GTAC GT^AC
AhdI 113 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 756 GGATC GGATC(4/5)
AocI 115 CCTAAGG CC^T[ACGT]AGG
Asp700I 453 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 113 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 664 GAGCTC G[AT]GC[AT]^C
AvaII 120 GGTCC G^G[AT]CC
BciVI 386 GTATCC GTATCC(6/5)
BcnI 669 CCGGG CC^[GC]GG
BfaI 442 CTAG C^TAG
BlpI 673 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 673 GCTCAGC GC^T[ACGT]AGC
BsaI 219 GGTCTC GGTCTC(1/5)
BsiHKAI 664 GAGCTC G[AT]GC[AT]^C
BsmFI 398 GTCCC GGGAC(10/14)
BspHI 627 TCATGA T^CATGA
BspMI 342 GCAGGT ACCTGC(4/8)
BstAPI 88 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 294 CGCG CG^CG
Bsu36I 115 CCTAAGG CC^T[ACGT]AGG
CelII 673 GCTCAGC GC^T[ACGT]AGC
DpnII 757 GATC ^GATC
DsaI 401 CCACGG C^C[GA][CT]GG
Eam1105I 113 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 651 GAAGAG CTCTTC(1/4)
Eco81I 115 CCTAAGG CC^T[ACGT]AGG
EcoRI 125 GAATTC G^AATTC
HapII 669 CCGG C^CGG
HgaI 692 GCGTC GACGC(5/10)
HpaII 669 CCGG C^CGG
Ksp632I 651 GAAGAG CTCTTC(1/4)
MaeI 442 CTAG C^TAG
MaeII 134 ACGT A^CGT
MboI 757 GATC ^GATC
MboII 651 GAAGA GAAGA(8/7)
MspI 669 CCGG C^CGG
MvnI 294 CGCG CG^CG
NciI 669 CCGGG CC^[GC]GG
NdeII 757 GATC ^GATC
RcaI 627 TCATGA T^CATGA
RsaI 139 GTAC GT^AC
SacI 664 GAGCTC GAGCT^C
SapI 651 GAAGAGC GCTCTTC(1/4)
Sau3AI 757 GATC ^GATC
SfcI 527 CTGTAG C^T[GA][CT]AG
SmlI 418 CTTGAG C^T[CT][GA]AG
TaqI 305 TCGA T^CGA
TfiI 624 GATTC G^A[AT]TC
TthHB8I 305 TCGA T^CGA
XmnI 453 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 293 CCGC CCGC(-3/-1)
591 CCGC
AcsI 125 GAATTC [GA]^AATT[CT]
431 AAATTC
ApoI 125 GAATTC [GA]^AATT[CT]
431 AAATTC
BanII 664 GAGCTC G[GA]GC[CT]^C
671 GGGCTC
BmyI 664 GAGCTC G[AGT]GC[ACT]^C
671 GGGCTC
BsaJI 215 CCCAGG C^C[ACGT][ACGT]GG
401 CCACGG
BsmAI 220 GTCTC GTCTC(1/5)
546 GTCTC
Bsp1286I 664 GAGCTC G[AGT]GC[ACT]^C
671 GGGCTC
BstF5I 600 CATCC GGATG(2/0)
657 CATCC
BstNI 216 CCAGG CC^[AT]GG
603 CCAGG
DrdI 348 GACTCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
685 GACCTTAGCGTC
EcoRII 216 CCAGG ^CC[AT]GG
603 CCAGG
FokI 600 CATCC GGATG(9/13)
657 CATCC
MseI 101 TTAA T^TAA
770 TTAA
MvaI 216 CCAGG CC^[AT]GG
603 CCAGG
PleI 348 GACTC GAGTC(4/5)
585 GAGTC
Tru9I 101 TTAA T^TAA
770 TTAA
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
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AluI BbvI BsiYI BslI BssKI
Cfr13I DdeI Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MnlI MwoI
NlaIII Sau96I ScrFI SfaNI TseI
Tsp45I Tsp509I TspRI