Restriction map for
pFN21AB6265
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BalI BamHI BanI BbeI
BbrPI BcgI BclI BglI BglII
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CfoI
Cfr10I CpoI DpnII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MboI MflI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 1314 CCGC CCGC(-3/-1)
AflII 1348 CTTAAG C^TTAAG
Alw44I 110 GTGCAC G^TGCAC
AlwNI 1945 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 110 GTGCAC G^TGCAC
AvaI 1855 CTCGAG C^[CT]CG[GA]G
AvrII 504 CCTAGG C^CTAGG
BbsI 285 GTCTTC GAAGAC(2/6)
BbvCI 907 GCTGAGG CCTCAGC(-5/-2)
BbvI 1539 GCAGC GCAGC(8/12)
BciVI 1627 GTATCC GTATCC(6/5)
BcnI 1027 CCCGG CC^[GC]GG
BfrI 1348 CTTAAG C^TTAAG
BlnI 504 CCTAGG C^CTAGG
BlpI 860 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 860 GCTCAGC GC^T[ACGT]AGC
BpuAI 285 GTCTTC GAAGAC(2/6)
BsaBI 1987 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 752 GAGACC GGTCTC(1/5)
BsgI 1562 CTGCAC GTGCAG(16/14)
BsmFI 1361 GGGAC GGGAC(10/14)
BsoBI 1855 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1157 TGTACA T^GTACA
BspDI 942 ATCGAT AT^CGAT
BsrGI 1157 TGTACA T^GTACA
BstAPI 1944 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 912 GGATG GGATG(2/0)
BstXI 884 CCATTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 860 GCTCAGC GC^T[ACGT]AGC
ClaI 942 ATCGAT AT^CGAT
DraI 1879 TTTAAA TTT^AAA
DraII 1518 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 235 GACATCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1123 CCACGG C^C[GA][CT]GG
EcoO109I 1518 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 827 GAATTC G^AATTC
Fnu4HI 1539 GCAGC GC^[ACGT]GC
FokI 912 GGATG GGATG(9/13)
HapII 1028 CCGG C^CGG
HincII 1432 GTTGAC GT[CT]^[GA]AC
HindII 1432 GTTGAC GT[CT]^[GA]AC
HpaII 1028 CCGG C^CGG
ItaI 1539 GCAGC GC^[ACGT]GC
MamI 1987 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1216 CAATTG C^AATTG
MslI 885 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1028 CCGG C^CGG
MunI 1216 CAATTG C^AATTG
NciI 1027 CCCGG CC^[GC]GG
PaeR7I 1855 CTCGAG C^TCGAG
PleI 1546 GAGTC GAGTC(4/5)
SacI 1852 GAGCTC GAGCT^C
SspBI 1157 TGTACA T^GTACA
TseI 1539 GCAGC G^C[AT]GC
XbaI 1291 TCTAGA T^CTAGA
XhoI 1855 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 11 enzymes) #
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BseRI 1193 CTCCTC GAGGAG(10/8)
1514 GAGGAG
EcoNI 1507 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1591 CCTGAAGTAGG
EcoT14I 504 CCTAGG C^C[AT][AT]GG
1273 CCTTGG
HindIII 608 AAGCTT A^AGCTT
1902 AAGCTT
HphI 508 GGTGA GGTGA(8/7)
1062 GGTGA
MaeII 9 ACGT A^CGT
148 ACGT
NlaIV 214 GGAACC GG[ACGT]^[ACGT]CC
2007 GGAGCC
SapI 931 GAAGAGC GCTCTTC(1/4)
1660 GAAGAGC
SspI 259 AATATT AAT^ATT
1066 AATATT
StyI 504 CCTAGG C^C[AT][AT]GG
1273 CCTTGG
Tsp45I 1328 GTGAC ^GT[GC]AC
1769 GTGAC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
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AcsI AfaI AluI ApoI Asp700I
AspHI AvaII BanII BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstNI Cac8I Cfr13I DdeI EarI
EcoRII HaeIII HinfI Ksp632I MaeI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII RsaI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I XmnI