Restriction map for
pFN21AE1259
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# NO CUT ENZYMES ( 100 enzymes) #
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AccI AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
AspI AviII AvrII BbeI BbrPI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DrdI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FspI
HincII HindII HindIII HpaI KasI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 787 GACGTC GACGT^C
Acc65I 1320 GGTACC G^GTACC
AcsI 1124 GAATTT [GA]^AATT[CT]
AhdI 1659 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 907 GTGCAC G^TGCAC
AocI 585 CCTGAGG CC^T[ACGT]AGG
ApaI 341 GGGCCC GGGCC^C
ApaLI 907 GTGCAC G^TGCAC
ApoI 1124 GAATTT [GA]^AATT[CT]
Asp718I 1320 GGTACC G^GTACC
AspEI 1659 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1308 TGGCCA TGG^CCA
BamHI 1166 GGATCC G^GATCC
BbvCI 1339 GCTGAGG CCTCAGC(-5/-2)
BclI 134 TGATCA T^GATCA
BfaI 151 CTAG C^TAG
BglI 583 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1050 CACGTA [CT]AC^GT[GA]
BsgI 264 CTGCAC GTGCAG(16/14)
BspMI 655 ACCTGC ACCTGC(4/8)
BsrDI 329 GCAATG GCAATG(2/0)
Bsu36I 585 CCTGAGG CC^T[ACGT]AGG
DraIII 1612 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1659 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 886 CTCTTC CTCTTC(1/4)
Eco81I 585 CCTGAGG CC^T[ACGT]AGG
FbaI 134 TGATCA T^GATCA
FseI 1209 GGCCGGCC GGCCGG^CC
HaeII 621 GGCGCT [GA]GCGC^[CT]
KpnI 1320 GGTACC GGTAC^C
Ksp632I 886 CTCTTC CTCTTC(1/4)
MaeI 151 CTAG C^TAG
MluNI 1308 TGGCCA TGG^CCA
MscI 1308 TGGCCA TGG^CCA
NotI 250 GCGGCCGC GC^GGCCGC
PpuMI 1562 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1267 CAGCTG CAG^CTG
SmaI 1177 CCCGGG CCC^GGG
SphI 524 GCATGC GCATG^C
SspI 202 AATATT AAT^ATT
TaqI 1019 TCGA T^CGA
TthHB8I 1019 TCGA T^CGA
XmaCI 1177 CCCGGG C^CCGGG
XmaI 1177 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 88 ACGTGT A^C[GA][CT]GT
1247 ACGTGT
AspHI 907 GTGCAC G[AT]GC[AT]^C
1201 GTGCTC
AvaI 1177 CCCGGG C^[CT]CG[GA]G
1465 CCCGAG
AvaII 1263 GGTCC G^G[AT]CC
1563 GGACC
BbsI 84 GAAGAC GAAGAC(2/6)
1021 GAAGAC
BcgI 824 CGAGTCTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1492 CGAGGCGTCTGC
BciVI 334 GGATAC GTATCC(6/5)
767 GTATCC
BpmI 1557 CTCCAG CTGGAG(16/14)
811 CTGGAG
BpuAI 84 GAAGAC GAAGAC(2/6)
1021 GAAGAC
BsiHKAI 907 GTGCAC G[AT]GC[AT]^C
1201 GTGCTC
BsmAI 297 GAGAC GTCTC(1/5)
673 GTCTC
BsmI 283 GCATTC GAATGC(1/-1)
934 GCATTC
BsoBI 1177 CCCGGG C^[CT]CG[GA]G
1465 CCCGAG
BsrBI 259 CCGCTC CCGCTC(-3/-3)
1472 GAGCGG
BstXI 803 CCATGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1755 CCACGAGGATGG
DraI 115 TTTAAA TTT^AAA
1032 TTTAAA
EcoT14I 803 CCATGG C^C[AT][AT]GG
1068 CCATGG
KspI 471 CCGCGG CCGC^GG
1637 CCGCGG
NcoI 803 CCATGG C^CATGG
1068 CCATGG
NspI 524 GCATGC [GA]CATG^[CT]
903 GCATGT
PstI 1330 CTGCAG CTGCA^G
1717 CTGCAG
SacII 471 CCGCGG CCGC^GG
1637 CCGCGG
SfcI 1330 CTGCAG C^T[GA][CT]AG
1717 CTGCAG
StyI 803 CCATGG C^C[AT][AT]GG
1068 CCATGG
TfiI 401 GAATC G^A[AT]TC
630 GATTC
Tsp509I 460 AATT ^AATT
1125 AATT
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp45I TspRI
Van91I XcmI XhoII