Restriction map for
pFN21AE6064
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BseAI BseRI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SfiI SfoI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 112 GTCTAC GT^[AC][GT]AC
AccII 27 CGCG CG^CG
AcsI 43 GAATTC [GA]^AATT[CT]
AgeI 730 ACCGGT A^CCGGT
AocI 912 CCTCAGG CC^T[ACGT]AGG
ApoI 43 GAATTC [GA]^AATT[CT]
Asp700I 598 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 256 CTAG C^TAG
BmyI 302 GTGCCC G[AGT]GC[ACT]^C
BsaAI 379 TACGTG [CT]AC^GT[GA]
BsaWI 730 ACCGGT [AT]^CCGG[AT]
BsgI 576 GTGCAG GTGCAG(16/14)
BsiEI 701 CGGTCG CG[GA][CT]^CG
BsmBI 860 CGTCTC CGTCTC(1/5)
Bsp1286I 302 GTGCCC G[AGT]GC[ACT]^C
BsrBI 519 CCGCTC CCGCTC(-3/-3)
BsrFI 730 ACCGGT [GA]^CCGG[CT]
BsrI 587 CCAGT ACTGG(1/-1)
BssSI 573 CTCGTG CACGAG(-5/-1)
BstAPI 469 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 27 CGCG CG^CG
Bsu36I 912 CCTCAGG CC^T[ACGT]AGG
Cfr10I 730 ACCGGT [GA]^CCGG[CT]
Eco81I 912 CCTCAGG CC^T[ACGT]AGG
EcoRI 43 GAATTC G^AATTC
EcoRV 550 GATATC GAT^ATC
EcoT14I 362 CCATGG C^C[AT][AT]GG
HaeII 147 GGCGCT [GA]GCGC^[CT]
HgaI 426 GCGTC GACGC(5/10)
HinfI 932 GATTC G^A[ACGT]TC
KspI 26 CCGCGG CCGC^GG
MaeI 256 CTAG C^TAG
MaeII 380 ACGT A^CGT
MaeIII 581 GTGAC ^GT[ACGT]AC
MspA1I 26 CCGCGG C[AC]G^C[GT]G
MvnI 27 CGCG CG^CG
NcoI 362 CCATGG C^CATGG
NspI 692 ACATGC [GA]CATG^[CT]
PinAI 730 ACCGGT A^CCGGT
PshAI 583 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 26 CCGCGG CCGC^GG
SexAI 116 ACCTGGT A^CC[AT]GGT
SfcI 879 CTACAG C^T[GA][CT]AG
SgrAI 729 CACCGGTG C[GA]^CCGG[CT]G
SmaI 20 CCCGGG CCC^GGG
StuI 456 AGGCCT AGG^CCT
StyI 362 CCATGG C^C[AT][AT]GG
TaqI 704 TCGA T^CGA
TfiI 932 GATTC G^A[AT]TC
Tsp45I 581 GTGAC ^GT[GC]AC
Tsp509I 44 AATT ^AATT
TthHB8I 704 TCGA T^CGA
XmaCI 20 CCCGGG C^CCGGG
XmaI 20 CCCGGG C^CCGGG
XmnI 598 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AlwI 156 GATCC GGATC(4/5)
687 GATCC
AvaI 20 CCCGGG C^[CT]CG[GA]G
275 CCCGAG
BsmAI 590 GTCTC GTCTC(1/5)
861 GTCTC
BsoBI 20 CCCGGG C^[CT]CG[GA]G
275 CCCGAG
EarI 91 CTCTTC CTCTTC(1/4)
607 CTCTTC
Ksp632I 91 CTCTTC CTCTTC(1/4)
607 CTCTTC
PpuMI 74 GGGACCT [GA]G^G[AT]CC[CT]
952 GGGTCCC
XcmI 248 CCAAGATGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
803 CCAGCTATGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BbvI BcnI BpmI BsaJI BsiYI
BslI BsmFI BspMI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI