Restriction map for
pFN21AE5921
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MroI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 478 CGCG CG^CG
AlwI 297 GATCC GGATC(4/5)
AvaII 747 GGTCC G^G[AT]CC
BcnI 816 CCGGG CC^[GC]GG
BglI 349 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 582 CTCCAG CTGGAG(16/14)
BsaI 607 GAGACC GGTCTC(1/5)
BsiEI 363 CGACCG CG[GA][CT]^CG
Bsp1407I 833 TGTACA T^GTACA
BsrDI 421 CATTGC GCAATG(2/0)
BsrFI 365 ACCGGC [GA]^CCGG[CT]
BsrGI 833 TGTACA T^GTACA
BstUI 478 CGCG CG^CG
BstXI 584 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 365 ACCGGC [GA]^CCGG[CT]
DpnII 297 GATC ^GATC
DsaI 415 CCATGG C^C[GA][CT]GG
EarI 54 CTCTTC CTCTTC(1/4)
EcoRV 211 GATATC GAT^ATC
EcoT14I 415 CCATGG C^C[AT][AT]GG
HinfI 683 GAATC G^A[ACGT]TC
Ksp632I 54 CTCTTC CTCTTC(1/4)
MboI 297 GATC ^GATC
MvnI 478 CGCG CG^CG
NciI 816 CCGGG CC^[GC]GG
NcoI 415 CCATGG C^CATGG
NdeII 297 GATC ^GATC
Sau3AI 297 GATC ^GATC
SfiI 348 GGCCTCTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 833 TGTACA T^GTACA
StyI 415 CCATGG C^C[AT][AT]GG
TaqI 362 TCGA T^CGA
TfiI 683 GAATC G^A[AT]TC
TthHB8I 362 TCGA T^CGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AciI 477 CCGC CCGC(-3/-1)
118 GCGG
AcsI 252 GAATTT [GA]^AATT[CT]
923 GAATTT
AocI 29 CCTCAGG CC^T[ACGT]AGG
876 CCTGAGG
ApoI 252 GAATTT [GA]^AATT[CT]
923 GAATTT
Asp700I 708 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
858 GAACCCTTTC
AspHI 130 GTGCTC G[AT]GC[AT]^C
676 GTGCTC
BmyI 130 GTGCTC G[AGT]GC[ACT]^C
676 GTGCTC
BsiHKAI 130 GTGCTC G[AT]GC[AT]^C
676 GTGCTC
Bsp1286I 130 GTGCTC G[AGT]GC[ACT]^C
676 GTGCTC
BspMI 341 ACCTGC ACCTGC(4/8)
656 ACCTGC
Bsu36I 29 CCTCAGG CC^T[ACGT]AGG
876 CCTGAGG
Cac8I 345 GCTGGC GC[ACGT]^[ACGT]GC
479 GCGTGC
Eco81I 29 CCTCAGG CC^T[ACGT]AGG
876 CCTGAGG
HapII 366 CCGG C^CGG
816 CCGG
HpaII 366 CCGG C^CGG
816 CCGG
HphI 249 GGTGA GGTGA(8/7)
597 GGTGA
MseI 524 TTAA T^TAA
913 TTAA
MspI 366 CCGG C^CGG
816 CCGG
NlaIV 164 GGCTCC GG[ACGT]^[ACGT]CC
727 GGCTCC
PflMI 588 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
698 CCAGGAAGTGG
PstI 70 CTGCAG CTGCA^G
692 CTGCAG
SfaNI 243 GATGC GCATC(5/9)
452 GCATC
StuI 710 AGGCCT AGG^CCT
784 AGGCCT
Tru9I 524 TTAA T^TAA
913 TTAA
Van91I 588 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
698 CCAGGAAGTGG
XcmI 359 CCATCGACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
584 CCAGCCAGATGGTGG
XmnI 708 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
858 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BalI BbvI BsaJI
BsiYI BslI BsmAI BssKI BstF5I
BstNI Cfr13I DdeI EaeI EcoRII
Fnu4HI FokI HaeIII ItaI MaeIII
MboII MluNI MnlI MscI MslI
MvaI MwoI NlaIII RsaI Sau96I
ScrFI SfcI TseI Tsp45I Tsp509I
TspRI