Restriction map for
pFN21AE4380
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 1161 AAATTT [GA]^AATT[CT]
AfaI 291 GTAC GT^AC
ApaI 945 GGGCCC GGGCC^C
ApoI 1161 AAATTT [GA]^AATT[CT]
Asp700I 198 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 102 GAGCTC G[AT]GC[AT]^C
BbeI 178 GGCGCC GGCGC^C
BbsI 838 GAAGAC GAAGAC(2/6)
BbvCI 613 CCTCAGC CCTCAGC(-5/-2)
BclI 1170 TGATCA T^GATCA
BglI 55 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 838 GAAGAC GAAGAC(2/6)
BsgI 901 GTGCAG GTGCAG(16/14)
BsiHKAI 102 GAGCTC G[AT]GC[AT]^C
BspMI 828 GCAGGT ACCTGC(4/8)
BssSI 43 CTCGTG CACGAG(-5/-1)
BstEII 525 GGTCACC G^GT[ACGT]ACC
BstPI 525 GGTCACC G^GT[ACGT]ACC
BstYI 767 AGATCC [GA]^GATC[CT]
CfoI 179 GCGC GCG^C
DrdI 958 GACCAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 182 CCGTGG C^C[GA][CT]GG
EaeI 50 TGGCCG [CT]^GGCC[GA]
EcoO65I 525 GGTCACC G^GT[ACGT]ACC
FauI 228 GCGGG CCCGC(4/6)
FbaI 1170 TGATCA T^GATCA
HaeII 178 GGCGCC [GA]GCGC^[CT]
HhaI 179 GCGC GCG^C
HinP1I 179 GCGC G^CGC
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
KasI 178 GGCGCC G^GCGCC
MaeII 674 ACGT A^CGT
MflI 767 AGATCC [GA]^GATC[CT]
NarI 178 GGCGCC GG^CGCC
PvuII 268 CAGCTG CAG^CTG
RsaI 291 GTAC GT^AC
SacI 102 GAGCTC GAGCT^C
SfoI 178 GGCGCC GGC^GCC
StuI 636 AGGCCT AGG^CCT
Tsp45I 526 GTCAC ^GT[GC]AC
XcmI 710 CCAGTTCCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 767 AGATCC [GA]^GATC[CT]
XmnI 198 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 17 GGCGTC G[GA]^CG[CT]C
178 GGCGCC
AlwI 768 GATCC GGATC(4/5)
131 GGATC
AlwNI 1024 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1070 CAGAATCTG
AvaII 117 GGACC G^G[AT]CC
153 GGTCC
BanI 178 GGCGCC G^G[CT][GA]CC
654 GGTGCC
BanII 102 GAGCTC G[GA]GC[CT]^C
945 GGGCCC
BmyI 102 GAGCTC G[AGT]GC[ACT]^C
945 GGGCCC
BsaHI 17 GGCGTC G[GA]^CG[CT]C
178 GGCGCC
BsaI 560 GAGACC GGTCTC(1/5)
956 GAGACC
BsmFI 797 GGGAC GGGAC(10/14)
693 GTCCC
Bsp1286I 102 GAGCTC G[AGT]GC[ACT]^C
945 GGGCCC
DraII 944 AGGGCCC [GA]G^G[ACGT]CC[CT]
945 GGGCCCT
EarI 520 GAAGAG CTCTTC(1/4)
857 GAAGAG
EcoO109I 944 AGGGCCC [GA]G^G[ACGT]CC[CT]
945 GGGCCCT
HgaI 18 GCGTC GACGC(5/10)
278 GCGTC
Hin1I 17 GGCGTC G[GA]^CG[CT]C
178 GGCGCC
Ksp632I 520 GAAGAG CTCTTC(1/4)
857 GAAGAG
MaeIII 526 GTCAC ^GT[ACGT]AC
683 GTAAC
MseI 367 TTAA T^TAA
1130 TTAA
MslI 345 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
648 CACAGAGGTG
MspA1I 226 CAGCGG C[AC]G^C[GT]G
268 CAGCTG
SfaNI 68 GCATC GCATC(5/9)
473 GCATC
Tru9I 367 TTAA T^TAA
1130 TTAA
Tsp509I 385 AATT ^AATT
1162 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI BbvI BcnI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfcI TfiI TseI TspRI