Restriction map for
pFN21AE3048
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# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MroI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 37 GTAC GT^AC
AluI 43 AGCT AG^CT
AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 8 GGTCC G^G[AT]CC
BalI 201 TGGCCA TGG^CCA
BbsI 216 GAAGAC GAAGAC(2/6)
BbvI 292 GCTGC GCAGC(8/12)
BcnI 5 CCCGG CC^[GC]GG
BfaI 313 CTAG C^TAG
BpuAI 216 GAAGAC GAAGAC(2/6)
BsaJI 488 CCTGGG C^C[ACGT][ACGT]GG
BsiYI 183 CCCAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 183 CCCAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 391 GAGAC GTCTC(1/5)
BspHI 512 TCATGA T^CATGA
BsrI 207 CCAGT ACTGG(1/-1)
BstF5I 417 CATCC GGATG(2/0)
BstYI 517 AGATCC [GA]^GATC[CT]
Cac8I 92 GCCTGC GC[ACGT]^[ACGT]GC
DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 201 TGGCCA [CT]^GGCC[GA]
EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 172 GAATTC G^AATTC
Fnu4HI 292 GCTGC GC^[ACGT]GC
FokI 417 CATCC GGATG(9/13)
HapII 6 CCGG C^CGG
HpaII 6 CCGG C^CGG
ItaI 292 GCTGC GC^[ACGT]GC
MaeI 313 CTAG C^TAG
MflI 517 AGATCC [GA]^GATC[CT]
MluNI 201 TGGCCA TGG^CCA
MscI 201 TGGCCA TGG^CCA
MseI 553 TTAA T^TAA
MspI 6 CCGG C^CGG
MwoI 59 GCTCTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 5 CCCGG CC^[GC]GG
NspI 361 GCATGT [GA]CATG^[CT]
RcaI 512 TCATGA T^CATGA
RsaI 37 GTAC GT^AC
SmlI 221 CTCAAG C^T[CT][GA]AG
Tru9I 553 TTAA T^TAA
TseI 292 GCTGC G^C[AT]GC
TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 53 CCATCTGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 517 AGATCC [GA]^GATC[CT]
XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AcsI 172 GAATTC [GA]^AATT[CT]
319 AAATTT
AlwI 249 GATCC GGATC(4/5)
518 GATCC
ApoI 172 GAATTC [GA]^AATT[CT]
319 AAATTT
BseRI 187 GAGGAG GAGGAG(10/8)
190 GAGGAG
DdeI 40 CTCAG C^T[ACGT]AG
80 CTCAG
EarI 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
Ksp632I 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
Tsp509I 173 AATT ^AATT
320 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BssKI BstNI Cfr13I DpnII EcoRII
HaeIII HinfI MboI MboII MnlI
MvaI NdeII NlaIII PleI Sau3AI
Sau96I ScrFI TfiI