Restriction map for
pFN21AE6665
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# NO CUT ENZYMES ( 158 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp700I Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmFI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI HinP1I HindIII KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 538 GACGTC GACGT^C
AciI 515 GCGG CCGC(-3/-1)
AcsI 416 AAATTC [GA]^AATT[CT]
AcyI 538 GACGTC G[GA]^CG[CT]C
AfaI 157 GTAC GT^AC
AflIII 678 ACATGT A^C[GA][CT]GT
AlwNI 296 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 740 GGGCCC GGGCC^C
ApoI 416 AAATTC [GA]^AATT[CT]
AseI 693 ATTAAT AT^TAAT
AsnI 693 ATTAAT AT^TAAT
AspI 394 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 728 TGGCCA TGG^CCA
BanII 740 GGGCCC G[GA]GC[CT]^C
BclI 387 TGATCA T^GATCA
BmyI 740 GGGCCC G[AGT]GC[ACT]^C
BpmI 17 CTGGAG CTGGAG(16/14)
BsaHI 538 GACGTC G[GA]^CG[CT]C
BsmAI 541 GTCTC GTCTC(1/5)
BsmBI 540 CGTCTC CGTCTC(1/5)
BsmI 718 GCATTC GAATGC(1/-1)
Bsp1286I 740 GGGCCC G[AGT]GC[ACT]^C
BspHI 390 TCATGA T^CATGA
BspLU11I 678 ACATGT A^CATGT
BstAPI 346 GCATACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 728 TGGCCA [CT]^GGCC[GA]
EcoT14I 505 CCTTGG C^C[AT][AT]GG
FbaI 387 TGATCA T^GATCA
Hin1I 538 GACGTC G[GA]^CG[CT]C
HpaI 231 GTTAAC GTT^AAC
MaeII 539 ACGT A^CGT
MaeIII 553 GTCAC ^GT[ACGT]AC
MluNI 728 TGGCCA TGG^CCA
MscI 728 TGGCCA TGG^CCA
MslI 391 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 394 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 693 ATTAAT AT^TAAT
PstI 701 CTGCAG CTGCA^G
RcaI 390 TCATGA T^CATGA
RsaI 157 GTAC GT^AC
SfcI 701 CTGCAG C^T[GA][CT]AG
StyI 505 CCTTGG C^C[AT][AT]GG
TaqI 564 TCGA T^CGA
Tsp45I 553 GTCAC ^GT[GC]AC
TspRI 704 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 394 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 564 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 249 GATCC GGATC(4/5)
444 GGATC
BcnI 252 CCCGG CC^[GC]GG
723 CCGGG
BsrDI 351 CATTGC GCAATG(2/0)
557 CATTGC
BsrI 16 ACTGG ACTGG(1/-1)
596 ACTGG
Cac8I 497 GCCAGC GC[ACGT]^[ACGT]GC
780 GCTTGC
DraII 739 AGGGCCC [GA]G^G[ACGT]CC[CT]
740 GGGCCCC
DsaI 39 CCACGG C^C[GA][CT]GG
151 CCACGG
EarI 490 GAAGAG CTCTTC(1/4)
648 GAAGAG
EcoO109I 739 AGGGCCC [GA]G^G[ACGT]CC[CT]
740 GGGCCCC
HapII 253 CCGG C^CGG
723 CCGG
HincII 231 GTTAAC GT[CT]^[GA]AC
682 GTCAAC
HindII 231 GTTAAC GT[CT]^[GA]AC
682 GTCAAC
HpaII 253 CCGG C^CGG
723 CCGG
HphI 517 GGTGA GGTGA(8/7)
527 TCACC
Ksp632I 490 GAAGAG CTCTTC(1/4)
648 GAAGAG
MspI 253 CCGG C^CGG
723 CCGG
MwoI 346 GCATACATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
501 GCAACCTTGGC
NciI 252 CCCGG CC^[GC]GG
723 CCGGG
NlaIV 740 GGGCCC GG[ACGT]^[ACGT]CC
741 GGCCCC
NspI 140 GCATGT [GA]CATG^[CT]
678 ACATGT
SfaNI 196 GATGC GCATC(5/9)
561 GCATC
TfiI 273 GATTC G^A[AT]TC
520 GAATC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboI MboII MnlI
MseI MvaI NdeII NlaIII PleI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I