Restriction map for
pFN21AE2619
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
Psp1406I RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AocI 1348 CCTCAGG CC^T[ACGT]AGG
AseI 468 ATTAAT AT^TAAT
AsnI 468 ATTAAT AT^TAAT
Asp700I 164 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 866 GTGCTC G[AT]GC[AT]^C
AvaII 923 GGTCC G^G[AT]CC
BbrPI 1249 CACGTG CAC^GTG
BclI 90 TGATCA T^GATCA
BlpI 651 GCTGAGC GC^T[ACGT]AGC
BmyI 866 GTGCTC G[AGT]GC[ACT]^C
BpmI 777 CTGGAG CTGGAG(16/14)
Bpu1102I 651 GCTGAGC GC^T[ACGT]AGC
BsiEI 1128 CGATCG CG[GA][CT]^CG
BsiHKAI 866 GTGCTC G[AT]GC[AT]^C
Bsp1286I 866 GTGCTC G[AGT]GC[ACT]^C
BspHI 675 TCATGA T^CATGA
Bsu36I 1348 CCTCAGG CC^T[ACGT]AGG
CelII 651 GCTGAGC GC^T[ACGT]AGC
DraII 922 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 164 GAAGAG CTCTTC(1/4)
Eco81I 1348 CCTCAGG CC^T[ACGT]AGG
EcoNI 53 CCTCTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 922 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1069 GAATTC G^AATTC
FbaI 90 TGATCA T^GATCA
Ksp632I 164 GAAGAG CTCTTC(1/4)
MwoI 562 GCAGTCCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 726 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1116 GAGTC GAGTC(4/5)
PmaCI 1249 CACGTG CAC^GTG
PmlI 1249 CACGTG CAC^GTG
PpuMI 922 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 886 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 468 ATTAAT AT^TAAT
PvuI 1128 CGATCG CGAT^CG
RcaI 675 TCATGA T^CATGA
SfaNI 1315 GATGC GCATC(5/9)
TaqI 1297 TCGA T^CGA
Tsp45I 1133 GTCAC ^GT[GC]AC
TthHB8I 1297 TCGA T^CGA
Van91I 726 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 164 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 304 GCGG CCGC(-3/-1)
574 GCGG
AflIII 129 ACGTGT A^C[GA][CT]GT
1248 ACACGT
AlwI 47 GGATC GGATC(4/5)
684 GGATC
BbsI 955 GAAGAC GAAGAC(2/6)
458 GTCTTC
BglII 277 AGATCT A^GATCT
781 AGATCT
BpuAI 955 GAAGAC GAAGAC(2/6)
458 GTCTTC
BsaAI 128 TACGTG [CT]AC^GT[GA]
1249 CACGTG
BsaBI 149 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
679 GATCTGGATC
BsaJI 29 CCTTGG C^C[ACGT][ACGT]GG
637 CCTTGG
BsmAI 853 GAGAC GTCTC(1/5)
229 GTCTC
BsmFI 1278 GGGAC GGGAC(10/14)
924 GTCCC
BsrI 762 ACTGG ACTGG(1/-1)
776 ACTGG
BssKI 691 CCAGG ^CC[ACGT]GG
1208 CCAGG
BstNI 691 CCAGG CC^[AT]GG
1208 CCAGG
BstYI 277 AGATCT [GA]^GATC[CT]
781 AGATCT
Cfr13I 923 GGTCC G^G[ACGT]CC
1205 GGGCC
EcoRII 691 CCAGG ^CC[AT]GG
1208 CCAGG
EcoT14I 29 CCTTGG C^C[AT][AT]GG
637 CCTTGG
HindIII 435 AAGCTT A^AGCTT
616 AAGCTT
MamI 149 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
679 GATCTGGATC
MflI 277 AGATCT [GA]^GATC[CT]
781 AGATCT
MspA1I 665 CAGCTG C[AC]G^C[GT]G
1269 CAGCTG
MvaI 691 CCAGG CC^[AT]GG
1208 CCAGG
PstI 785 CTGCAG CTGCA^G
1282 CTGCAG
PvuII 665 CAGCTG CAG^CTG
1269 CAGCTG
Sau96I 923 GGTCC G^G[ACGT]CC
1205 GGGCC
ScaI 476 AGTACT AGT^ACT
759 AGTACT
ScrFI 691 CCAGG CC^[ACGT]GG
1208 CCAGG
SfcI 785 CTGCAG C^T[GA][CT]AG
1282 CTGCAG
StyI 29 CCTTGG C^C[AT][AT]GG
637 CCTTGG
XhoII 277 AGATCT [GA]^GATC[CT]
781 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsiYI BslI BstF5I Cac8I
DdeI DpnII EcoRV Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI NdeII NlaIII NlaIV
RsaI Sau3AI TfiI Tru9I TseI
Tsp509I TspRI XcmI