Restriction map for
pFN21AE5911
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 492 CCGC CCGC(-3/-1)
AflIII 285 ACATGT A^C[GA][CT]GT
AluI 748 AGCT AG^CT
AseI 111 ATTAAT AT^TAAT
AsnI 111 ATTAAT AT^TAAT
AspI 871 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 183 CCTCAGC CCTCAGC(-5/-2)
BpmI 202 CTGGAG CTGGAG(16/14)
BsaAI 763 TACGTG [CT]AC^GT[GA]
BsmAI 634 GTCTC GTCTC(1/5)
BspLU11I 285 ACATGT A^CATGT
DdeI 184 CTCAG C^T[ACGT]AG
DsaI 314 CCATGG C^C[GA][CT]GG
EaeI 684 TGGCCG [CT]^GGCC[GA]
EcoRI 28 GAATTC G^AATTC
HapII 687 CCGG C^CGG
HinfI 843 GAATC G^A[ACGT]TC
HpaI 828 GTTAAC GTT^AAC
HpaII 687 CCGG C^CGG
HphI 722 TCACC GGTGA(8/7)
MfeI 535 CAATTG C^AATTG
MspA1I 747 CAGCTG C[AC]G^C[GT]G
MspI 687 CCGG C^CGG
MunI 535 CAATTG C^AATTG
MwoI 740 GCTATGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 314 CCATGG C^CATGG
NspI 285 ACATGT [GA]CATG^[CT]
PflFI 871 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 52 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 111 ATTAAT AT^TAAT
PstI 160 CTGCAG CTGCA^G
PvuII 747 CAGCTG CAG^CTG
SapI 252 GCTCTTC GCTCTTC(1/4)
SfcI 160 CTGCAG C^T[GA][CT]AG
SmlI 664 CTCAAG C^T[CT][GA]AG
TfiI 843 GAATC G^A[AT]TC
TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 871 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 28 GAATTC [GA]^AATT[CT]
501 AAATTC
AlwNI 402 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
677 CAGCCTCTG
ApoI 28 GAATTC [GA]^AATT[CT]
501 AAATTC
Asp700I 693 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
843 GAATCCTTTC
BfaI 17 CTAG C^TAG
40 CTAG
BstYI 206 AGATCC [GA]^GATC[CT]
668 AGATCC
DraII 52 GGGTCCC [GA]G^G[ACGT]CC[CT]
884 AGGCCCT
EcoO109I 52 GGGTCCC [GA]G^G[ACGT]CC[CT]
884 AGGCCCT
EcoT14I 57 CCAAGG C^C[AT][AT]GG
314 CCATGG
HincII 828 GTTAAC GT[CT]^[GA]AC
914 GTCAAC
HindII 828 GTTAAC GT[CT]^[GA]AC
914 GTCAAC
MaeI 17 CTAG C^TAG
40 CTAG
MaeII 156 ACGT A^CGT
764 ACGT
MflI 206 AGATCC [GA]^GATC[CT]
668 AGATCC
SfaNI 237 GATGC GCATC(5/9)
263 GATGC
StyI 57 CCAAGG C^C[AT][AT]GG
314 CCATGG
XhoII 206 AGATCC [GA]^GATC[CT]
668 AGATCC
XmnI 693 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
843 GAATCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AlwI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cfr13I DpnII EarI EcoRII
Fnu4HI FokI HaeIII ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI Tru9I
TseI Tsp45I Tsp509I