Restriction map for
pFN21AE1612
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tru9I Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 122 GAATTC [GA]^AATT[CT]
AcyI 268 GACGCC G[GA]^CG[CT]C
AfaI 252 GTAC GT^AC
AlwNI 305 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 318 GGGCCC GGGCC^C
ApoI 122 GAATTC [GA]^AATT[CT]
AspHI 165 GAGCAC G[AT]GC[AT]^C
AvaII 243 GGTCC G^G[AT]CC
AvrII 292 CCTAGG C^CTAGG
BbvI 98 GCTGC GCAGC(8/12)
BfaI 293 CTAG C^TAG
BlnI 292 CCTAGG C^CTAGG
BsaHI 268 GACGCC G[GA]^CG[CT]C
BsiHKAI 165 GAGCAC G[AT]GC[AT]^C
BsiYI 228 CCCCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 228 CCCCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 352 ACCTGC ACCTGC(4/8)
BstXI 299 CCAGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 128 CTGAG C^T[ACGT]AG
DpnII 189 GATC ^GATC
EcoRI 122 GAATTC G^AATTC
EcoRV 332 GATATC GAT^ATC
Fnu4HI 98 GCTGC GC^[ACGT]GC
HgaI 268 GACGC GACGC(5/10)
Hin1I 268 GACGCC G[GA]^CG[CT]C
HphI 194 TCACC GGTGA(8/7)
ItaI 98 GCTGC GC^[ACGT]GC
MaeI 293 CTAG C^TAG
MaeIII 22 GTAAC ^GT[ACGT]AC
MboI 189 GATC ^GATC
MspA1I 151 CAGCTG C[AC]G^C[GT]G
NdeII 189 GATC ^GATC
PvuII 151 CAGCTG CAG^CTG
RsaI 252 GTAC GT^AC
Sau3AI 189 GATC ^GATC
SmlI 175 CTCAAG C^T[CT][GA]AG
StuI 6 AGGCCT AGG^CCT
TseI 98 GCTGC G^C[AT]GC
Tsp509I 123 AATT ^AATT
TspRI 305 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AluI 145 AGCT AG^CT
152 AGCT
BanII 72 GGGCTC G[GA]GC[CT]^C
318 GGGCCC
BstF5I 63 CATCC GGATG(2/0)
84 CATCC
Cac8I 4 GCAGGC GC[ACGT]^[ACGT]GC
209 GCCAGC
DraII 317 AGGGCCC [GA]G^G[ACGT]CC[CT]
318 GGGCCCT
EcoO109I 317 AGGGCCC [GA]G^G[ACGT]CC[CT]
318 GGGCCCT
EcoT14I 39 CCTTGG C^C[AT][AT]GG
292 CCTAGG
FokI 63 CATCC GGATG(9/13)
84 CATCC
NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC
318 GGGCCC
NspI 101 GCATGT [GA]CATG^[CT]
169 ACATGC
StyI 39 CCTTGG C^C[AT][AT]GG
292 CCTAGG
XcmI 210 CCAGCAGTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
327 CCAGAGATATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BmyI BsaJI Bsp1286I BssKI BstNI
Cfr13I EcoRII HaeIII MboII MnlI
MvaI NlaIII Sau96I ScrFI