##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DpnII       DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoRV       
EcoT14I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HphI        
ItaI        KasI        KpnI        KspI        MamI        
MboI        MfeI        MflI        MluI        MroI        
MspA1I      MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspI        StyI        SwaI        
TseI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI            4  GTAGAC               GT^[AC][GT]AC
AfaI          168  GTAC                 GT^AC
Asp700I       303  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          55  GAGCAC               G[AT]GC[AT]^C
BalI           49  TGGCCA               TGG^CCA
BbvCI        1103  CCTCAGC              CCTCAGC(-5/-2)
BmyI           55  GAGCAC               G[AGT]GC[ACT]^C
BsaAI        1370  CACGTA               [CT]AC^GT[GA]
BsaJI         324  CCGTGG               C^C[ACGT][ACGT]GG
BsgI         1367  CTGCAC               GTGCAG(16/14)
BsiHKAI        55  GAGCAC               G[AT]GC[AT]^C
Bsp1286I       55  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I      167  TGTACA               T^GTACA
BsrDI        1337  GCAATG               GCAATG(2/0)
BsrGI         167  TGTACA               T^GTACA
BstF5I        153  GGATG                GGATG(2/0)
Cac8I         341  GCATGC               GC[ACGT]^[ACGT]GC
DraI         1009  TTTAAA               TTT^AAA
DsaI          324  CCGTGG               C^C[GA][CT]GG
EaeI           49  TGGCCA               [CT]^GGCC[GA]
EarI          634  GAAGAG               CTCTTC(1/4)
EcoT22I       155  ATGCAT               ATGCA^T
FokI          153  GGATG                GGATG(9/13)
Ksp632I       634  GAAGAG               CTCTTC(1/4)
MaeII        1371  ACGT                 A^CGT
MaeIII       1418  GTGAC                ^GT[ACGT]AC
MluNI          49  TGGCCA               TGG^CCA
MscI           49  TGGCCA               TGG^CCA
NsiI          155  ATGCAT               ATGCA^T
RsaI          168  GTAC                 GT^AC
SfaNI         154  GATGC                GCATC(5/9)
SphI          341  GCATGC               GCATG^C
SspBI         167  TGTACA               T^GTACA
TaqI          685  TCGA                 T^CGA
Tsp45I       1418  GTGAC                ^GT[GC]AC
TthHB8I       685  TCGA                 T^CGA
XmnI          303  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AgeI          509  ACCGGT               A^CCGGT
              593  ACCGGT              
BfaI          380  CTAG                 C^TAG
             1220  CTAG                
BsaWI         509  ACCGGT               [AT]^CCGG[AT]
              593  ACCGGT              
BsrFI         509  ACCGGT               [GA]^CCGG[CT]
              593  ACCGGT              
Cfr10I        509  ACCGGT               [GA]^CCGG[CT]
              593  ACCGGT              
HapII         510  CCGG                 C^CGG
              594  CCGG                
HinfI          70  GATTC                G^A[ACGT]TC
              687  GATTC               
HpaII         510  CCGG                 C^CGG
              594  CCGG                
MaeI          380  CTAG                 C^TAG
             1220  CTAG                
MspI          510  CCGG                 C^CGG
              594  CCGG                
NspI          157  GCATGT               [GA]CATG^[CT]
              341  GCATGC              
PinAI         509  ACCGGT               A^CCGGT
              593  ACCGGT              
StuI          668  AGGCCT               AGG^CCT
             1088  AGGCCT              
TfiI           70  GATTC                G^A[AT]TC
              687  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AcsI        AluI        ApoI        AvaII       BpmI        
BsiYI       BslI        BsrI        Cfr13I      DdeI        
EcoRI       HaeIII      HindIII     MboII       MnlI        
MseI        MslI        NdeI        NlaIII      Sau96I      
Tru9I       Tsp509I     TspRI