Restriction map for
pFN21AE6382
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeII
MluI MroI MspA1I NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 578 CGCG CG^CG
AcsI 590 GAATTC [GA]^AATT[CT]
AflIII 251 ACATGT A^C[GA][CT]GT
ApoI 590 GAATTC [GA]^AATT[CT]
AspHI 55 GAGCTC G[AT]GC[AT]^C
AvaII 333 GGACC G^G[AT]CC
AviII 140 TGCGCA TGC^GCA
BalI 323 TGGCCA TGG^CCA
BamHI 912 GGATCC G^GATCC
BanII 55 GAGCTC G[GA]GC[CT]^C
BclI 479 TGATCA T^GATCA
BglII 895 AGATCT A^GATCT
BmyI 55 GAGCTC G[AGT]GC[ACT]^C
BsaBI 896 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 79 CTGCAC GTGCAG(16/14)
BsiHKAI 55 GAGCTC G[AT]GC[AT]^C
BsmFI 775 GGGAC GGGAC(10/14)
BsmI 752 GAATGC GAATGC(1/-1)
Bsp1286I 55 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 629 TGTACA T^GTACA
BspLU11I 251 ACATGT A^CATGT
BspMI 386 GCAGGT ACCTGC(4/8)
BsrDI 965 CATTGC GCAATG(2/0)
BsrGI 629 TGTACA T^GTACA
BstAPI 960 GCAACCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 578 CGCG CG^CG
DraII 317 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 610 CCATGG C^C[GA][CT]GG
EaeI 323 TGGCCA [CT]^GGCC[GA]
EarI 891 GAAGAG CTCTTC(1/4)
EcoO109I 317 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 590 GAATTC G^AATTC
EcoT14I 610 CCATGG C^C[AT][AT]GG
FauI 174 GCGGG CCCGC(4/6)
FbaI 479 TGATCA T^GATCA
FspI 140 TGCGCA TGC^GCA
HindIII 261 AAGCTT A^AGCTT
Ksp632I 891 GAAGAG CTCTTC(1/4)
MamI 896 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 372 CAATTG C^AATTG
MluNI 323 TGGCCA TGG^CCA
MscI 323 TGGCCA TGG^CCA
MseI 266 TTAA T^TAA
MunI 372 CAATTG C^AATTG
MvnI 578 CGCG CG^CG
NcoI 610 CCATGG C^CATGG
PstI 559 CTGCAG CTGCA^G
SacI 55 GAGCTC GAGCT^C
SbfI 558 CCTGCAGG CCTGCA^GG
SphI 845 GCATGC GCATG^C
Sse8387I 558 CCTGCAGG CCTGCA^GG
SspBI 629 TGTACA T^GTACA
StyI 610 CCATGG C^C[AT][AT]GG
TfiI 351 GATTC G^A[AT]TC
Tru9I 266 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 704 GTCTAC GT^[AC][GT]AC
832 GTAGAC
AciI 207 CCGC CCGC(-3/-1)
174 GCGG
AlwNI 241 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
292 CAGGCACTG
BsaI 31 GAGACC GGTCTC(1/5)
19 GGTCTC
BsmAI 31 GAGAC GTCTC(1/5)
20 GTCTC
BsrFI 16 GCCGGT [GA]^CCGG[CT]
233 ACCGGC
BsrI 396 ACTGG ACTGG(1/-1)
488 ACTGG
Cfr10I 16 GCCGGT [GA]^CCGG[CT]
233 ACCGGC
Cfr13I 318 GGGCC G^G[ACGT]CC
333 GGACC
HphI 99 GGTGA GGTGA(8/7)
116 TCACC
MaeIII 494 GTAAC ^GT[ACGT]AC
689 GTAAC
MwoI 134 GCTTTCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
960 GCAACCATTGC
NlaIV 317 GGGGCC GG[ACGT]^[ACGT]CC
912 GGATCC
PleI 3 GACTC GAGTC(4/5)
835 GACTC
Sau96I 318 GGGCC G^G[ACGT]CC
333 GGACC
ScaI 221 AGTACT AGT^ACT
921 AGTACT
SfaNI 151 GATGC GCATC(5/9)
284 GCATC
StuI 200 AGGCCT AGG^CCT
555 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BpmI BsaJI BsiYI BslI BssKI
BstF5I BstNI BstYI Cac8I CfoI
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MboI MboII
MflI MnlI MslI MspI MvaI
NdeII NlaIII NspI RsaI Sau3AI
ScrFI SfcI TseI Tsp509I TspRI
XhoII