Restriction map for
pFN21AE4009
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbvCI BclI BfrI BglI BlnI
BsaBI BsaI BseAI BsiWI BsmBI
BsmI Bsp1407I BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 1931 GGCGCC G[GA]^CG[CT]C
Alw44I 1735 GTGCAC G^TGCAC
Aor51HI 1922 AGCGCT AGC^GCT
ApaLI 1735 GTGCAC G^TGCAC
AviII 1772 TGCGCA TGC^GCA
BalI 710 TGGCCA TGG^CCA
BbeI 1931 GGCGCC GGCGC^C
BbsI 1234 GTCTTC GAAGAC(2/6)
BcgI 1148 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2402 GTATCC GTATCC(6/5)
BlpI 1662 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1662 GCTCAGC GC^T[ACGT]AGC
BpuAI 1234 GTCTTC GAAGAC(2/6)
BsaHI 1931 GGCGCC G[GA]^CG[CT]C
BsaWI 1142 ACCGGA [AT]^CCGG[AT]
BspDI 2413 ATCGAT AT^CGAT
BspLU11I 626 ACATGT A^CATGT
BssSI 1762 CACGAG CACGAG(-5/-1)
BstAPI 1335 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1662 GCTCAGC GC^T[ACGT]AGC
ClaI 2413 ATCGAT AT^CGAT
DsaI 2204 CCGTGG C^C[GA][CT]GG
EagI 1561 CGGCCG C^GGCCG
EclXI 1561 CGGCCG C^GGCCG
Eco47III 1922 AGCGCT AGC^GCT
Eco52I 1561 CGGCCG C^GGCCG
FspI 1772 TGCGCA TGC^GCA
Hin1I 1931 GGCGCC G[GA]^CG[CT]C
KasI 1931 GGCGCC G^GCGCC
MaeIII 498 GTCAC ^GT[ACGT]AC
MluNI 710 TGGCCA TGG^CCA
MscI 710 TGGCCA TGG^CCA
NarI 1931 GGCGCC GG^CGCC
PaeR7I 1438 CTCGAG C^TCGAG
SbfI 150 CCTGCAGG CCTGCA^GG
SfoI 1931 GGCGCC GGC^GCC
SmaI 854 CCCGGG CCC^GGG
SphI 254 GCATGC GCATG^C
Sse8387I 150 CCTGCAGG CCTGCA^GG
StuI 1397 AGGCCT AGG^CCT
TfiI 426 GAATC G^A[AT]TC
Tsp45I 498 GTCAC ^GT[GC]AC
XhoI 1438 CTCGAG C^TCGAG
XmaCI 854 CCCGGG C^CCGGG
XmaI 854 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 937 GTCTAC GT^[AC][GT]AC
2359 GTCTAC
AflIII 452 ACGTGT A^C[GA][CT]GT
626 ACATGT
BbrPI 1168 CACGTG CAC^GTG
1981 CACGTG
BfaI 196 CTAG C^TAG
247 CTAG
BglII 1367 AGATCT A^GATCT
1583 AGATCT
BsiEI 1561 CGGCCG CG[GA][CT]^CG
1881 CGACCG
BsrBI 1572 CCGCTC CCGCTC(-3/-3)
1558 GAGCGG
BsrDI 290 GCAATG GCAATG(2/0)
326 GCAATG
BstXI 285 CCAAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
698 CCACTGAGATGG
EcoRI 13 GAATTC G^AATTC
102 GAATTC
EcoT14I 285 CCAAGG C^C[AT][AT]GG
2105 CCTTGG
MaeI 196 CTAG C^TAG
247 CTAG
NaeI 304 GCCGGC GCC^GGC
1928 GCCGGC
NgoMIV 304 GCCGGC G^CCGGC
1928 GCCGGC
PmaCI 1168 CACGTG CAC^GTG
1981 CACGTG
PmlI 1168 CACGTG CAC^GTG
1981 CACGTG
StyI 285 CCAAGG C^C[AT][AT]GG
2105 CCTTGG
XcmI 698 CCACTGAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
884 CCAAGCGCAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII XmnI