Restriction map for
pFN21AE4174
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AvaII AviII AvrII BamHI BbrPI
BclI BfrI BglI BlnI BsaBI
BseAI BseRI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BstBI Bsu36I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HpaI Ksp632I MamI
MfeI MluI MroI MslI MunI
NaeI NcoI NgoMIV NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
StyI SwaI XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 411 GTATAC GT^[AC][GT]AC
AlwNI 1480 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 646 ATTAAT AT^TAAT
AsnI 646 ATTAAT AT^TAAT
BbeI 1644 GGCGCC GGCGC^C
BbvCI 1494 CCTCAGC CCTCAGC(-5/-2)
BcgI 934 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1196 GGATAC GTATCC(6/5)
BglII 756 AGATCT A^GATCT
BlpI 1530 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1530 GCTGAGC GC^T[ACGT]AGC
BsaI 1016 GAGACC GGTCTC(1/5)
BsaWI 1411 ACCGGA [AT]^CCGG[AT]
BsmBI 1677 CGTCTC CGTCTC(1/5)
BspMI 1653 GCAGGT ACCTGC(4/8)
BssHII 1363 GCGCGC G^CGCGC
Bst1107I 411 GTATAC GTA^TAC
BstEII 1407 GGTGACC G^GT[ACGT]ACC
BstPI 1407 GGTGACC G^GT[ACGT]ACC
BstXI 170 CCAACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 411 GTATAC GTA^TAC
CelII 1530 GCTGAGC GC^T[ACGT]AGC
EcoO65I 1407 GGTGACC G^GT[ACGT]ACC
HindIII 1039 AAGCTT A^AGCTT
KasI 1644 GGCGCC G^GCGCC
KspI 1268 CCGCGG CCGC^GG
NarI 1644 GGCGCC GG^CGCC
NdeI 377 CATATG CA^TATG
NheI 1378 GCTAGC G^CTAGC
NotI 1274 GCGGCCGC GC^GGCCGC
PflMI 1328 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 646 ATTAAT AT^TAAT
PvuII 1564 CAGCTG CAG^CTG
SacI 1768 GAGCTC GAGCT^C
SacII 1268 CCGCGG CCGC^GG
SfoI 1644 GGCGCC GGC^GCC
SphI 1149 GCATGC GCATG^C
TaqI 1837 TCGA T^CGA
TthHB8I 1837 TCGA T^CGA
Van91I 1328 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 232 GGTACC G^GTACC
781 GGTACC
Asp718I 232 GGTACC G^GTACC
781 GGTACC
AspI 220 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
799 GACCTGGTC
BalI 362 TGGCCA TGG^CCA
1205 TGGCCA
BbsI 1117 GAAGAC GAAGAC(2/6)
1344 GAAGAC
BpmI 1306 CTGGAG CTGGAG(16/14)
1354 CTGGAG
BpuAI 1117 GAAGAC GAAGAC(2/6)
1344 GAAGAC
BsaAI 1315 TACGTG [CT]AC^GT[GA]
1546 TACGTG
BsmFI 966 GGGAC GGGAC(10/14)
990 GTCCC
BsmI 90 GAATGC GAATGC(1/-1)
697 GCATTC
BsrFI 1108 GCCGGT [GA]^CCGG[CT]
1624 ACCGGC
BssSI 1258 CTCGTG CACGAG(-5/-1)
1473 CTCGTG
Cfr10I 1108 GCCGGT [GA]^CCGG[CT]
1624 ACCGGC
DsaI 1268 CCGCGG C^C[GA][CT]GG
1601 CCGTGG
EagI 1275 CGGCCG C^GGCCG
1395 CGGCCG
EclXI 1275 CGGCCG C^GGCCG
1395 CGGCCG
Eco52I 1275 CGGCCG C^GGCCG
1395 CGGCCG
EcoRV 40 GATATC GAT^ATC
343 GATATC
HaeII 1644 GGCGCC [GA]GCGC^[CT]
1683 GGCGCT
KpnI 232 GGTACC GGTAC^C
781 GGTACC
MluNI 362 TGGCCA TGG^CCA
1205 TGGCCA
MscI 362 TGGCCA TGG^CCA
1205 TGGCCA
NspI 515 GCATGT [GA]CATG^[CT]
1149 GCATGC
PflFI 220 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
799 GACCTGGTC
SexAI 800 ACCTGGT A^CC[AT]GGT
1703 ACCTGGT
SmaI 1505 CCCGGG CCC^GGG
1865 CCCGGG
SspI 529 AATATT AAT^ATT
769 AATATT
StuI 357 AGGCCT AGG^CCT
975 AGGCCT
Tth111I 220 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
799 GACCTGGTC
XmaCI 1505 CCCGGG C^CCGGG
1865 CCCGGG
XmaI 1505 CCCGGG C^CCGGG
1865 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
BanI BanII BbvI BcnI BfaI
BmyI BsaHI BsaJI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII